천연 식물 추출물의 항염 효과가 in vitro 반추위 발효성상과 메탄 생성에 미치는 영향
Anti-inflammatory Effect of Natural Plant Extracts on in vitro Rumen Fermentation and Methane Emission
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본 시험은 항염에 효과가 있다고 알려진 식물 추출물의 첨가가 반추위 발효와 메탄 생성에 미치는 영향을 알아보기 위해 in vitro 실험을 수행하였다. 항염과 항산화 효과가 있는 산뽕나무, 뽕나무, 예덕나무, 오동나무, 방아풀과 은행나무 6종을 선발하였다. 반추위액과 McDougall buffer 혼합액 15mL과 티모시 0.3g, 각각의 추출물을 기질의 5%로 넣고 39℃에서 3, 9, 12, 24, 48 그리고 72시간 배양하였다. 항염 효과가 있는 식물 추출물의 첨가는 반추위 발효 성상(pH, 건물소화율, Glucose 농도, 암모니아농도, 단백질 농도, 미생물 성장량, 휘발성지방산)에는 영향을 미치지 않았다. 총 가스 발생량은 각기다른 양상을 보였으며, 이산화탄소 발생량은 48시간대에서 예덕나무와 방아풀에서 대조구에 비해 유의적(p<0.05)으로 높았다. 또한 메탄 발생량은 배양 초기에는 대조구보다 첨가구에서 유의적(p<0.05)으로감소하였으나, 발효가 진행될수록 대조구에 비해 첨가구에서 더 많은 메탄이 발생하였다. Polyphenol과flavonoid는 은행나무 추출물구에서 가장 높았다. 본 시험의 결과에서 항염에 효과가 있는 식물 추출물을 in vitro 반추위 배양액에 첨가하였을 때, 반추위 발효에는 영향을 미치지 않았고, 메탄은 초기 발효에 저감 효과가 있는 것으로 사료된다.
Abstract
This study was conducted to investigate the effects of anti-inflammatory plant extracts on thein vitro rumen fermentation characteristics and methane emission. Anti-inflammatory plantextracts from Morus bombycis Koidz, Mallotus japonicus L., Morus alba L., Paulownia coreanaUyeki, Isodon japonicus Hara and Ginkgo biloba L. were used in the study. The ruminalfluid(5 mL), McDougall buffer(10 mL), timothy as a substrate(0.3 g) and each anti-inflammatoryplant extract(5% of substrate) were dispensed anaerobically into 50mL serum bottle. Themixtures were incubated for 3, 9, 12, 24, 48 and 72h at 39℃ without shaking. Supplementationof the anti-inflammatory plant extracts did not effects characteristics(pH, digestibility of drymatter, glucose concentration, ammonia concentration, protein concentration, VFA) on rumenfermentation. Total gas was showed a different pattern depending on treatments. Carbon dioxidewas significantly(p<0.05) higher in Morus alba and Isodon japonicus than in control at 48h. Methane was significantly(p<0.05) lower in treatment than in control at initial fermentation. However the more incubation time was increased, the more methane emission was higher intreatment than in control. The concentrations of polyphenol and flavonoid were higher in Ginkgobiloba. In conclusion, supplementation of the anti-inflammatory plant extracts did not effect onrumen fermentation and methane emission was decreased in initial fermentation.
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