갈색종피 및 Penta Null 유전자형을 가진 콩 계통 육종
Breeding of Soybean Strain with Brown Seed Coat and Penta Null Genotype
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콩 종실에는 레시틴, 아이소플라본, 사포닌, 루테인, 안토시아닌 및 플라보노이드와 같은 생리활성 성분과 약 40%의 단백질이 들어 있어 최근식물성 대체 단백질의 주요 재료로 이용성이 점차 증가되고 있다. 그러나 lipoxygenase, Kunitz Trypsin Inhibitor (KTI), lectin, 7Sα′subunit단백질 및 stachyose와 같은 알레르기를 유발시키고 품질 및 기능성을 저하시키는 성분들도 존재한다. 본 연구는 갈색 종피와 penta null 유전자형(lox1lox2lox3tilecgy1rs2)을 가져 성숙 종실에서 lipoxygenase, KTI, lectin 및 7Sα′subunit의 4가지 단백질이 모두 없으면서 stachyose의 함량이매우 낮은 콩 계통을 육성하기 위하여 진행되었다. 5개의 자원을 이용하여 창성된 육종집단으로부터 갈색 종피와 penta null 유전자형을 가진6개의 F2 종자가 선발되었다. 농업형질이 양호한 계통을 선발하여 F7 계통에서 lipoxygenase, KTI, lectin 및 7Sα' subunit의 4가지 단백질에대한 부재를 확인하였다. 선발계통은 갈색종피와 자주색 꽃 및 유한신육형이며 배꼽색은 흰색이고 성숙 자엽색은 노란색이었다. 선발계통의 경장은68cm 정도이며 백립중은 29.2g으로 대립이었고 stachyose의 함량은 3.8g/kg으로 매우 낮았으며 수량은 2.75ton/ha 정도였다. 선발된 계통은성숙 콩 종실에서 콩 가공적성과 품질 및 기능성을 저하시키는 lipoxygenase, KTI, lectin 및 7Sα′subunit의 4가지 단백질이 모두 없으면서 난소화성당으로 알려진 stachyose의 함량이 매우 낮은 유색콩 품종 및 중간모본으로 이용될 수 있을 것으로 사료되었다.
Abstract
Soybean [Glycine max (L.) Merr.] seed is a major protein source for production of plant-based meat. However, antinutritional factorssuch as stachyose, lipoxygenase, Kunitz Trypsin Inhibitor (KTI), lectin and 7S α’subunit proteins exist in mature seed. The geneticremoval of these antinutritional components is necessary step in soybean breeding. The research was conducted to improve new soybeanstrain with brown seed coat and penta null genotype (lox1lox2lox3tilecgy1rs2) for lipoxygenase, KTI, lectin, 7S α' subunit and stachyose. Six F2 seeds with brown seed coat and penta null genotype were selected from the breeding population derived from five genotypes. The absence for lipoxygenase, KTI, lectin and 7S α' subunit proteins was confirmed in F7 strain developed. The breeding line has purpleflower, determinant growth type, brown seed coat, white hilum color and yellow cotyledon. Stem height of new strain was 68 cm and100-seed weight was 29.2 g. The content of stachyose was very low at 3.8 g/kg and the yield was about 2.75 ton/ha. A strain developedthrough this study can be registered as new cultivar and can be used as intermediate parent to improve soybean cultivars with pentanull genotype and colored seed coat.
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