Kunitz Trypsin Inhibitor 단백질 결핍 검정콩 계통의 선발
Selection of Black Soybean Line with Absence of Kunitz Trypsin Inhibitor Protein
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초록
최근 콩에 대한 기능성 성분이 중요시되면서 anthocyanin을 함유한 검정콩 품종에 대한 요구가 증가하고 있다. 콩의 기능성을 높이기 위하여 antinutritional 물질인 kunitz trypsin inhibitor 단백질이 결핍된 검정콩 품종을 육성하기 위한 KTI 단백질 결핍 검정콩 계통을 선발하기 위하여 본 실험을 수행하여 얻어진 결과를 요약하면 다음과 같다. 검정 녹색자엽 국 내 재래종인 “20M183”과 KTI 단백질이 결핍되어 있는 “C242”(titi)의 교배로부터 얻어진 F2 집단에서 검정종피 녹색자엽 및 kunitz trypsin inhibitor 단백질이 결핍된 두 개체 선발하였다. 선발된 F2 유래 F3 와 F4식물체에서 김정 종피색, 녹색자엽, kunitz trypsin inhibitor 단백질 결핍 형질이 고정되었으며 포장에서 고정된 F4 식물체로부터 조사된 초장은 35 cm 백립중은 20 g이었다. 선발 고정된 계통은 KTI 단백질 결핍 검정콩 육종 모본으로 활용될 수 있을 것으로 보인다.
Abstract
Soybean kunitz trypsin inhibitor protein is responsible for the inferior nutritional quality of unheated or incompletelyheated soybean meal. The objective of this research was to select black seed coated soybean lines with absence of kunitz trypsininhibitor protein. Black soybean genotype 20M183 (Ti , kunitz trypsin inhibitor protein present, green cotyledon) was crossedwith genotype C242 (titi, kunitz trypsin inhibitor protein absent, yellow seed coat and cotyledon). Presence and absence of kunitztrypsin inhibitor protein was tested by SDS electrophoresis. Two F2kunitz trypsin inhibitor were selected from the F2 population. Each 10 F3 plant from these F2 plant selected was advanced. Seed coatcolor, cotyledon color and kunitz trypsin inhibitor protein were also tested after harvesting. Several F4 seeds with black seed coat,green cotyledon and absence of kunitz trypsin inhibitor were planted to F4 population (F5 seed). Plant height, seed coat color, coty-ledon color, seed weight, and kunitz trypsin inhibitor protein were estimated on the F5 plants. Several F5 plants with black seed coat,green cotyledon, 30 cm plant height, medium seed weight (20 g/100 seeds), and absence of kunitz trypsin inhibitor protein wereztrypsin inhibitor protein.
참고문헌 (10)
- "Birk Y 1961. Purification and some properties of a highly active inhibitor of trypsin and chymotrypsin from soybeans. Biochem. Biophys. Acta 54", pp.378~381
- "Specht JE 1999. An integrated genetic linkage map of the soybean genome. Crop Sci. 39", pp.1464~1490
- "Steiner RF 1968. Soybean inhibitor. I. Separations and some properties of three inhibitors from commercial crude soybean trypsin inhibitors. Biochemistry 7", pp.521~530
- "Hymowitz T 1980. Inheritance of an acid phosphatase and its linkage with the Kunitz trypsin inhibitor in seed protein of soybeans. Crop Sci. 20", pp.83~85
- "Hadley HH 1972. Inheritance of a trypsin inhibitor variant in seed protein of soybeans. Crop Sci. 12"
- "Kiang YT 1987. Mapping three protein loci on a soybean chromosome Crop Sci. 27", pp.44~46
- "Kunitz M 1945. Crystallization of a soybean trypsin inhibitor from soybean. Science 101", pp.668~669
- "Hymowitz T 1979. Inheritance of the absence of the Kunitz trypsin inhibitor in seed protein of soybeans. Crop Sci. 19", pp.107~109
- "Hadley HH 1969. Genetic differences in soybean trypsin inhibitors separated by disc electrophoresis. Crop Sci. 9", pp.489~491
- "Hauge SM 1948. The nutritive quality and the trypsin inhibitor content of soybean flour heated at various temperatures. J. Nutr. 35", pp.379~389