연잎 추출물의 구강미생물에 대한 항균 효과
Antibacterial effect on leaf-extract from Nelumbo nucifera against oral microorganism
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초록
Objectives : The purpose of the study is to investigate the activities of Nelumbo nuciferaleaf extracts on Streptococcus mutans, Streptococcus mitis, Streptococcus sobrinus, Porphyromonasgingivalis, Prevotella intermedia, and Treponema denticola. Methods : The inhibitory effect of lotus leaf extracts on the growth of oral bacteria was assessedin experiments with extracts from freshly harvested and pulverized lotus leaves and bacterial culturesof dental caries. Results : The results showed that N. nucifera extracts possess antimicrobial activity on all bacterialstrains. The minimal inhibitory concentration (MIC) values varied from 4 mg/ml to 10 mg/mlagainst antimicrobial activity. The relative growth ratio (RGR) against of N. nucifera extracts weredetermined as 50% in concentration of 4.0 mg/ml. The extract of N. nucifera was effective inreducing on the glucosyltransferase (GTase) activity of six strains in vitro. Conclusions : Methanol extracts of lotus leaves showed antimicrobial effects on three bacterialspecies causing dental caries and three bacterial species causing periodontitis, as well as inhibitoryeffects on GTase activity.
Abstract
Objectives : The purpose of the study is to investigate the activities of Nelumbo nuciferaleaf extracts on Streptococcus mutans, Streptococcus mitis, Streptococcus sobrinus, Porphyromonasgingivalis, Prevotella intermedia, and Treponema denticola. Methods : The inhibitory effect of lotus leaf extracts on the growth of oral bacteria was assessedin experiments with extracts from freshly harvested and pulverized lotus leaves and bacterial culturesof dental caries. Results : The results showed that N. nucifera extracts possess antimicrobial activity on all bacterialstrains. The minimal inhibitory concentration (MIC) values varied from 4 mg/ml to 10 mg/mlagainst antimicrobial activity. The relative growth ratio (RGR) against of N. nucifera extracts weredetermined as 50% in concentration of 4.0 mg/ml. The extract of N. nucifera was effective inreducing on the glucosyltransferase (GTase) activity of six strains in vitro. Conclusions : Methanol extracts of lotus leaves showed antimicrobial effects on three bacterialspecies causing dental caries and three bacterial species causing periodontitis, as well as inhibitoryeffects on GTase activity.
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