(-)-Epigalocatechin의 Fusobacterium nucleatum, Prevotella intermedia 및 Porphyromonas gingivalis에 대한 항균 효과
Antimicrobial effect of (-)-epigalocatechin on Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis
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초록
The aim of this study was to investigate the antimicrobial effect of (-)-epigalocatechin on Fusobacterium nucleatum, Prevotella intermedia, and Porphyromonas gingivalis. To test the antimicrobial effect of (-)-epigalocatechin,the minimum inhibitory concentration (MIC) of against 4 strains of F. nucleatum, 2 strains of P. intermedia, and 2 strains of P. gingivalis was measured by broth dilution method. Time-kill curves were assessed for susceptible bacteria, testing 0×MIC (control group), 0.5×MIC, 1×MIC, and 2×MIC for (-)-epigalocatechin, by counting viable bacteria after 3, 90,180, 360, 720, 1440 minutes. The MIC of (-)-epigalocatechin was 0.312-0.625, 0.625, and 0.625 mg/ml on the strains of F. nucleatum, P. intermedia, and P. gingivalis, respectively. Time-kill curves demonstrated (-)-epigalocatechin had bactericidal activity on P. intermedia ATCC 25611T, P. gingival is ATCC 53978, and F. nucleatum subsp. fusiforme ATCC 51190T. The results suggest that (-)-epigalocatechin can be useful in developing the oral hygiene product such as tooth past and gargling solution for the prevention of periodontal diseases.
Abstract
The aim of this study was to investigate the antimicrobial effect of (-)-epigalocatechin on Fusobacterium nucleatum, Prevotella intermedia, and Porphyromonas gingivalis. To test the antimicrobial effect of (-)-epigalocatechin,the minimum inhibitory concentration (MIC) of against 4 strains of F. nucleatum, 2 strains of P. intermedia, and 2 strains of P. gingivalis was measured by broth dilution method. Time-kill curves were assessed for susceptible bacteria, testing 0×MIC (control group), 0.5×MIC, 1×MIC, and 2×MIC for (-)-epigalocatechin, by counting viable bacteria after 3, 90,180, 360, 720, 1440 minutes. The MIC of (-)-epigalocatechin was 0.312-0.625, 0.625, and 0.625 mg/ml on the strains of F. nucleatum, P. intermedia, and P. gingivalis, respectively. Time-kill curves demonstrated (-)-epigalocatechin had bactericidal activity on P. intermedia ATCC 25611T, P. gingival is ATCC 53978, and F. nucleatum subsp. fusiforme ATCC 51190T. The results suggest that (-)-epigalocatechin can be useful in developing the oral hygiene product such as tooth past and gargling solution for the prevention of periodontal diseases.
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