Candida tropicalis의 역학적 형별 검사를 위한Infrequent-Restriction-Site PCR법의 평가
Evaluation of Infrequent-Restriction-Site PCR forEpidemiological Typing of Candida tropicalis
1
조회
0
다운로드
0
인용
0
참고문헌
초록
배경: Candida tropicalis의 역학적 형별검사로 분자생물학적 형별검사인 infrequent-restriction-site PCR (IRS-PCR)법의 유용 성을 평가하였다. 방법: IRS-PCR법의 유용성을 평가하기 위해 pulsed-field gel electrophoresis (PFGE)를 통해 형별이 판별된 산발적으로 분리된 8균주와 6가지 클론에 속해 있는 클론성 24균주로 구성된 총 32주의 C. tropicalis 균주를 IRS-PCR법으로 분석하였다. Candida 균종에 대한 IRS-PCR 방법의 종간 분별력을 평가하기 위해 Candida glabrata와 Candida krusei, Candida albicans, Candida parapsilosis의 4균종으로 구성된 20균주를 함께 검사하였다. 결과: IRS-PCR법으로는 산발적인 균주들과 클론성 균주들을 감별할 수 없었다. 동일한 클론에 속해 있는 대부분의 균주들이 서로 다른 IRS-PCR형이나 군집으로 분류되었고 일부의 서로 다른 산발적인 균주들이 동일한 IRS-PCR형으로 분류되었다. C. tropicalis 분리주들을 대상으로 각 균주들의 양상간 변이도를 Dice 계수로 측정한 결과 쌍간 유사도는 75.4∼100%이었다. 반면 Candida의 5균종들을 대상으로 각 균종들의 양상간 변이도를 측정한 Dice 계수는 25∼69.2%였다. 그래서 다섯 가지 Candida 균종을 서로 쉽게 구별할 수 있었다. 결론: IRS-PCR의 형별 결과는 PFGE에 비견할 만하지 못하여 C. tropicalis의 역학적 형별검사 도구로 쓰기에는 부적합한것으로 여겨졌다. 저자들이 아는 바로는 본고가 C. tropicalis의 역학적 형별검사법으로서 IRS-PCR법의 유용성을 살펴본 첫 번째 평가 논문이다.
Abstract
Background: We evaluated the usefulness of a newly developed molecular typing method of infrequent restriction site polymerase chain reaction (IRS-PCR) as an epidemiological DNA fingerprinting tool for Candida tropicalis. Methods: Thirty-two strains of C. tropicalis comprising eight sporadic strains and 24 clonal strains belonging to six clones, of which clonal type were previously confirmed by pulsed-field gel electrophoresis (PFGE), were tested by IRS-PCR to evaluate the usefulness of this technique. Twenty strains of Candida species, including C. glabrata, C. krusei, C. albicans, and C.mparapsilosis, were also tested to assess the ability of IRS-PCR to discriminate among species of Candida. Results: Using the IRS-PCR assay, sporadic strains of C. tropicalis could not be differentiated from clonal strains. Most strains belonging to the same clones were classified as different IRS-PCR types or clusters, and some different sporadic strains were classified as the same IRS-PCR types. When pattern variation was examined for different strains of C. tropicalis using IRS-PCR, pairwise similarity measured by the Dice coefficient was 75.4∼100%. In contrast, pairwise similarity among isolates of five different species of Candida was 25∼69.2%. Therefore, five different species of Candida were easily differentiated. Conclusion: The IRS-PCR typing assay appears to be an inadequate tool for the epidemiological typing of C. tropicalis, because the typing result of IRSPCR is not comparable to that of PFGE. To our knowledge, this is the first evaluation study for IRSPCR as an epidemiological typing tool for C. tropicalis.
참고문헌 (20)
- "Nosocomial fungaemia: a 2-year prospective study", 45, pp.69~72 (2000)
- "Nosocomial candidemia in a tertiary care hospital in Saudi Arabia", 153, pp.195~198 (2002)
- "Stable susceptibility of Candida blood isolates to fluconazole despite increasing use during the past 10 years", 52, pp.71~77 (2003)
- "The epidemiology of hematogenous candidiasis caused by diferent Candida species", 24, pp.1122~1128 (1997)
- "Variations in DNA subtype and antifungal susceptibility among clinical isolates of Candida tro-picalis", 27, pp.63~67 (1997)
- "Development of two species-specific fingerprinting probes for broad computer-assisted epidemiological studies of Candida tropicalis", 34, pp.3063~3071 (1996)
- "DNA fingerprinting by infrequent-restriction-site amplification", 34, pp.2386~2390 (1996)
- "Diferentiation of pathogenic Bar-tonella species by infrequent restriction site PCR", 38, pp.3010~3015 (2000)
- "Molecular Typing of Urinary Isolates of Candida tropicalis by pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) Analysis", 33, pp.392~403 (2001)
- "and isoenzyme analysis of Trypanosoma rangeli strains", pp.261~7
- "Epidemiologic analysis of sporadic Salmonella typhi isolates and those from outbreaks by pulsed-field gel electrophoresis", pp.1135~41
- "Discriminatory power of typing schemes based on Simpson's index of diversity for Neisseria gonorrhoeae", pp.2831~3
- "Comparative analysis of infrequent-restriction-site PCR and pulsed-field gel electrophoresis for epidemiological typing of Legionella pneumophila serogroup 1 strains", 36, pp.161~167 (1998)
- "Application of infrequent-restriction-site PCR to clinical isolates of Acinetobacter baumanni and Serratia marcescens", 37, pp.3108~3112 (1999)
- "Molecular epidemiology of nosocomial infection associated with multi-resistant Acinetobacter baumannii by infrequent-restriction- site PCR", 51, pp.27~32 (2002)
- "Characterization of Listeria monocytogenes strains involved in invasive and noninvasive listeriosis outbreaks by PCR-based fingerprinting techniques", 67, pp.1793~1799 (2001)
- "Suitability of PCR fingerprinting, infrequent-restriction-site PCR, and pulsed-field gel electrophoresis, combined with computerized gel analysis, in library typing of Salmonella enterica serovar enteritidis", 66, pp.5273~5281 (2000)
- Dillon B, "Limited diversity among human isolates of Bartonella henselae", 40, pp.4691~4699 (2002)
- Choi TY, "Application of infrequent-restriction-site amplification for genotyping of mycobacterium tuberculosis and non-tuberculous Mycobacterium", 17, pp.593~598 (2002)
- Huang YC, "Genotyping analysis of colonizing candidal isolates from very-low-birthweight infants in a neonatal intensive care unit", 58(3), pp.200~203 (2004)