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rRNA Spacer 부위를 이용한 Candida의 균종동정

Identification of Candida Species by Analysis of rRNA Spacer Regions

김혜련|이미경|노병인
대한의진균학회 (The Korean Society for Medical Mycology)|2006.03|대한의진균학회지 (Journal of Mycology and Infection)|제11권 제1호|pp. 13-18

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참고문헌

주제분류미생물학

초록

Abstract

Background: The genus Candida comprises 163 species, the most common pathogen in the genus is C. albicans, however, other Candida species are considered as emergent pathogens. Because there are species-specific differences in the susceptibility of Candida spp. to the currently used therapeutic drugs, species identification is critical for therapeutic planning and accurate epidemiological records. The objective of this study is to evaluate the performance of tRNA intergenic length polymorphism (tDNA-ILP) analysis for the accurate identification of Candida species. Methods: For the identifying the 7 type strains and 54 clinical isolates of Candida, the suitability of tDNA-ILP was evaluated. The polymerase chain reaction (PCR) using a pair of primers or a single primer was performed. The PCR products were separated by electrophoresis in 2% agarose gels for 70 min. Results: In seven Candida type strains, tDNA-ILP using a single primer (reverse) can be easily analyzed by visual comparision. Fifty-three of 54 strains were identified as the same species with conventional identification. Conclusions: The tDNA-ILP analysis can be useful for the simple and rapid identification of Candida species in routine laboratories.

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