Infrequent-Restriction-Site 중합효소 연쇄 반응(ISR-PCR)을 사용 한 지역사회 발생 Extended Spectrum Beta-Lactamase (ESBL) 생성 대장균(Escherichia coli )의 분자역학적 분석 및 Pulsedfield gel electrophoresis (PFGE)와의 비교
Molecular Epidemiologic Analysis of Community- Onset Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli Using Infrequent-Restriction- Site Polymerase Chain Reaction (IRS-PCR) with Comparison by Pulsed-Field Gel Electrophoresis (PFGE)
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Background: We evaluated the ability of infrequent restriction site-polymerase chain reaction (IRS-PCR) to perform molecular epidemiologic analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli , and also assessed the use of PFGE as an alternative method. Materials and Methods: IRS-PCR assay was performed using combinations of adaptors for XbaI and HhaI restriction sites on clinical isolates of E. coli (n=51). We compared the discriminatory power, quality and efficiency of IRS-PCR to PFGE. Results: In E. coli , PFGE discriminated 39 (76.4%) and IRS-PCR discerned 41 (80.3%)of the total 51 strains. It took much less time to complete IRS-PCR (one day) than PFGE (at least 4 days). Conclusions: IRS-PCR is a more sensitive and rapid alternative to PFGE for molecular epidemiologic analysis of E. coli .
Abstract
Background: We evaluated the ability of infrequent restriction site-polymerase chain reaction (IRS-PCR) to perform molecular epidemiologic analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli , and also assessed the use of PFGE as an alternative method. Materials and Methods: IRS-PCR assay was performed using combinations of adaptors for XbaI and HhaI restriction sites on clinical isolates of E. coli (n=51). We compared the discriminatory power, quality and efficiency of IRS-PCR to PFGE. Results: In E. coli , PFGE discriminated 39 (76.4%) and IRS-PCR discerned 41 (80.3%)of the total 51 strains. It took much less time to complete IRS-PCR (one day) than PFGE (at least 4 days). Conclusions: IRS-PCR is a more sensitive and rapid alternative to PFGE for molecular epidemiologic analysis of E. coli .
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