본문 바로가기
KERD
논문KCI 등재

간세포암의 p53 코돈 249의 돌연변이와 과발현 빈도 및 HBV DNA 양성률

Frequency of Mutation of Codon 249, Overexpression ofp53, and Hepatitis B Virus DNA Positivityin Hepatocellular Carcinoma

김성환|신성희|김진희|김동민|박영진|이미자|전호종|장숙진|문대수|박건|비두루
대한임상미생물학회 (Korean Society of Clinical Microbiology)|2007.10|Annals of Clinical Microbiology|제10권 제2호|pp. 84-89

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

ImmunohistochemistryHepatocellular carcinomaHepatitis B vi-rusp53 mutationp53 overexpression

초록

Background: In hepatocellular carcinoma (HCC), the frequency of p53 mutation and the association with hepatitis B virus (HBV) infection varies with geographic locations and risk factors. The aim of this study was to determine the frequency of codon 249 mutation of p53, p53 overexpression, and HBV DNA positivity and to observe the relationship between them in Korean HCC. Methods: We analyzed overexpression of p53 in hepatoma tissue from 17 HCC patients by immunohistochemistry (IHC), specific mutations at the third base position of codon 249 by PCR-restriction fragment length polymorphism (PCR-RFLP) method, and presence of HBV by nested PCR. Results: Although a point mutation at codon 250 was seen in one (5.8%) of 17 patients, no codon 249 mutations were found in the patient cohort. The p53 protein was overexpressed in 4 (23.5%) of 17 HCCs. PCR for HBV DNA from HCCs showed a positivity rate of 82.4% (14 of 17 specimens). Conclusion: In HCC of this study, HBV infection was not associated with either 249 mutation or overexpression of p53, and overexpression of p53 protein seemed to be related to other than this mutation.

Abstract

Background: In hepatocellular carcinoma (HCC), the frequency of p53 mutation and the association with hepatitis B virus (HBV) infection varies with geographic locations and risk factors. The aim of this study was to determine the frequency of codon 249 mutation of p53, p53 overexpression, and HBV DNA positivity and to observe the relationship between them in Korean HCC. Methods: We analyzed overexpression of p53 in hepatoma tissue from 17 HCC patients by immunohistochemistry (IHC), specific mutations at the third base position of codon 249 by PCR-restriction fragment length polymorphism (PCR-RFLP) method, and presence of HBV by nested PCR. Results: Although a point mutation at codon 250 was seen in one (5.8%) of 17 patients, no codon 249 mutations were found in the patient cohort. The p53 protein was overexpressed in 4 (23.5%) of 17 HCCs. PCR for HBV DNA from HCCs showed a positivity rate of 82.4% (14 of 17 specimens). Conclusion: In HCC of this study, HBV infection was not associated with either 249 mutation or overexpression of p53, and overexpression of p53 protein seemed to be related to other than this mutation.

참고문헌 (32)

  1. "Hepatocellular carcinoma prevention:A worldwide emergence between the opulence of developed countries and the economic constraints of developing nations", 12, pp.7239~7249 (2006)
  2. "Epidemiological characteristics and risk factors of hepatocellular carcinoma", 12, pp.S294~S308 (1997)
  3. "Hepatitis B and alterations of the p53 tumor suppressor gene in hepatocellular carcinoma", 17, pp.1244~51 (1993)
  4. "Plasma antioxidant vitamins, chronic hepatitis B virus infection and urinary aflatoxin B1-DNA adducts in healthy males", 18, pp.1189~1194 (1997)
  5. "The gene for human p53 celular tumor antigen is located on chromosome 17 short arm(17p13)", 83, pp.130~4 (1986)
  6. "Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein", 5, pp.151~9 (1991)
  7. "Mutations in the p53tumor suppressor gene:clues to cancer etiology and mole-cular pathogenesis", 54, pp.4855~78 (1994)
  8. "p53 tumor suppressor gene mutations in hepatocellular carcinoma patients in India", 88, pp.1565~1573 (2000)
  9. "Is p53 gene mutation an indicatior of the biological behaviors of recurence of hepatocellular carcinoma?", 9, pp.1202~1207 (2003)
  10. "p53 in tumour pathology:can we trust immunohistochemistry-Revisited!", 172, pp.1~4 (1994)
  11. "p53gene status and expression of p53, mdm2, and p21Waf1", Cip1 proteins in colorectal cancer, 2000, pp.437, 241~247
  12. "Serologically silent hepatitis B virus coinfection in patients with hepatitis C virus-associated chronic liver disease: clinical and virological significance", 58, pp.201~207 (1999)
  13. "p53 function and dysfunction", 70, pp.523~6 (1992)
  14. "High levels of p53 protein expression do not correlate with p53 mutations in hepatocellular carcinoma", 11, pp.502~510 (2004)
  15. "Imunohi-stochemical detection of aberrant p53 expression in hepatocellular carcinoma: correlation with cell proliferative activity indices,inclu-ding mitotic index and MIB-1 immunostaining", 26, pp.326~333 (1995)
  16. "Expression of the G1-S modulators in hepatitis B virus-related hepatocellular carcinoma and dysplastic nodule:association of cyclin D1 and p53 proteins with the progression of hepatocellular carcinoma", 16, pp.424~432 (2001)
  17. "p53 and its downstream proteins as molecular targets of cancer", 45, pp.409~415 (2006)
  18. "Geographic characterization of hepatitis virus infections, genoty-ping of hepatitis B virus, and p53 mutation in hepatocelular carcinoma analyzed by in situ detection of viral genomes from carcinoma tissues: comparison among six diferent countries", 56, pp.12~18 (2003)
  19. Wild CP, "The toxicology of aflatoxins as a basis for public health decisions", 17, pp.471~481 (2002)
  20. Soussi T, "The TP53 Web Site"
  21. "Hepatocellular carcinoma in Korea:introduction and overview", 45, pp.217~226 (2005)
  22. "Prevalence of viral hepatitis markers in Korean patients with hepatocellular carcinoma", 13, pp.306~310 (1998)
  23. "The clinical report of 1,078 cases of hepatocellular carcinomas: National Cancer Center experience", 10, pp.288~297 (2004)
  24. "Hepatocellular carcinoma", 28, pp.1161~1165 (1998)
  25. "Rising incidence of hepatocellular carcinoma in the United States", 340, pp.745~750 (1999)
  26. "Hepatocellular ca-rcinoma:an update", 25, pp.497~516 (2001)
  27. "TP53 and liver carcinogenesis", 21, pp.201~216 (2003)
  28. "Genetic alterations and oncogenic pathways in hepatocellular carcinoma", 963, pp.21~36 (2002)
  29. "Overexpression of p53 protein is not directly related to hepatitis B x protein expression and is associated with neoplastic progression in hepatocellular carcinomas rather than hepatic preneoplasia", 462, pp.365~380 (2000)
  30. "Aberrations of p53 gene in human hepatocellular carcinoma from China", 14, pp.169~73 (1993)
  31. "p53 overexpression is frequent in European hepatocellular carcinoma and largely independent of the codon 249 hot spot mutation", 9, pp.195~204 (1994)
  32. "The expression of p53 antigen in primary malignant epithelial tumors of the liver Overexpression of p53 and HBV in HC 89", pp.172~6

관련 논문AI추천

한국인의 폐암에서 TP53 유전자 변이 분석신경철, 이관호, 장희경, 전창호, 정상봉, 최석철대한진단검사의학회 · Laboratory Medicine Online2011 · 제1권 제4호Adeno-Associated Virus 2-Mediated Hepatocellular Carcinoma is Very Rare in Korean Patients권준혁, 김종원, 박경진, 박준희, 이종안, 조재원대한진단검사의학회 · Annals of Laboratory Medicine2016 · 제36권 제5호간세포암종에서 Thrombospondin-1 및 -2의 발현: 종양 혈관신생 및 p53 과발현과의 연관성강명재, 문우성, 박호성, 손현진, 정재신대한병리학회 · Journal of Pathology and Translational Medicine2005 · 제39권 제4호Targeted Next-Generation Sequencing of Plasma Cell-Free DNA in Korean Patients with Hepatocellular CarcinomaChae Hyojin, Choi Hayoung, Kang Dain, Kim Myungshin, Kim Yonggoo, Kwon Ahlm, Sung Pil Soo, Yoon Seung Kew대한진단검사의학회 · Annals of Laboratory Medicine2021 · 제41권 제2호Characterization and Prognostic Value of Mutations in Exons 5 and 6 of the p53 Gene in Patients with Colorectal Cancers in Central IranMehdi Nikbakht, Mohammad H. Derakhshan, Mohammad J. Namazi, Mohammad Salehi, Rahim Golmohammadi거트앤리버 소화기연관학회협의회 · Gut and Liver2013 · 제7권 제3호Nuclear Expression of Hepatitis B Virus X Protein Is Associated with Recurrence of Early-Stage Hepatocellular Carcinomas: Role of Viral Protein in Tumor Recurrence서경석, 이경분, 이규호, 이남준, 장자준, 정혜연, Jing Jin대한병리학회 · Journal of Pathology and Translational Medicine2016 · 제50권 제3호

이 간행물의 다른 논문 (17건)

BK 바이러스 DNA 정량을 위한 TaqMan Probe법 실시간중합효소연쇄반응법의 수행능 평가최병후, 정희영, 성흥섭, 황경아, 박숙자, 김미나, 김유리pp. 77-83임상검체에서 분리된 칸디다 균종에서2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride 비색법을 이용한Fluconazole 항진균제 감수성 검사의 평가김재현, 이은정, 김혜란, 장철훈, 신정환, 이자영, 이정녀pp. 90-95Candida tropicalis의 역학적 형별 검사를 위한Infrequent-Restriction-Site PCR법의 평가박건, 신성희, 박영진, 신종희, 장숙진, 문대수, 한호림, 문영래pp. 96-101국내 의료기관의 호흡기바이러스 검사현황 및 5개 대학병원에서검출된 호흡기바이러스 역학이규만, 이남용, 김의종, 이창규, 강정옥pp. 102-108전남지역 주민에서 마이코플라스마 폐렴에 대한 항체가 분포권순석, 강연호, 전두영, 장미정, 배송미pp. 109-113
원문 보기