Evaluation of the Vitek 2 AST-N055 Card for the Susceptibility Testing of Acinetobacter baumannii Isolates to Amikacin
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초록
We collected 76 clinical isolates of Acinetobacter baumannii (amikacin MIC by Vitek 2 AST-N055 card: ≤2μg/mL, 11 isolates; 4μg/mL, 19 isolates; 8μg/ mL, 17 isolates; 16μg/mL, 27 isolates; and ≥64μg/ mL, 2 isolates) from a university hospital and evaluated the Vitek 2 AST-N055 card vs the broth microdilution as a reference method for testing susceptibility to amikacin. Vitek 2 AST-N055 card yielded very major errors in 15 isolates (19.7%) and minor errors in 26 isolates (34.2%). Of the 15 isolates shown very major errors, 14 had Vitek 2 MICs ranging from 8 to 16μg/mL. The results of our study suggest strongly that it is unreliable to test the amikacin susceptibility by Vitek 2 AST-N055 card of A. baumannii with the Vitek 2 MICs ranging from 8 to 16μg/mL. In those cases, another susceptibility test, such as broth microdilution (BMD), should be performed to confirm the results.
Abstract
76주의 Acinetobacter baumannii (Vitek 2 AST-N055 card의 amikacin MIC: ≤2μg/mL, 11 isolates; 4μg/mL, 19 isolates; 8μg/ mL, 17 isolates; 16μg/mL, 27 isolates; ≥64μg/mL, 2 isolates)를 대상으로 액체배지 미량희석법을 표준방법으로 하여 Vitek 2 AST-N055 카드의 amikacin 감수성시험의 정확성을 평가하고자 하였다. Vitek 2 AST-N055 카드는 19.7% (15주)가 very major error이었고 34.2% (26주)가 minor error이었다. Very major error를 보인 15주의 A. baumannii 중 14주의 Vitek 2 MIC는 8∼16μg/mL이었다. 결과적으로 Vitek 2 AST-N055 card의 A. baumannii에 대한 amikacin MIC가 8∼16μg/mL를 보이는 결과는 부정확하여 미량액체배지희석법과 같은 확인검사가 필요하다.
참고문헌 (7)
- Centers for Disease Control and Prevention, "Acinetobacter baumannii infections among patients at military medical facilities treating injured U. S. service members, 2002-2004", MMWR Morb Mortal Wkly Rep, 53, pp.1063~1066 (2004)
- Jeon BC, "Investigation of a nosocomial outbreak of imipenem-resistant Acinetobacter baumannii producing the OXA-23 β-lactamase in Korea", J Clin Microbiol, 43, pp.2241~2245 (2005)
- Sunenshine RH, "Multidrug-resistant Acinetobacter infection mortality rate and length of hospitalization", Emerg Infect Dis, 13, pp.97~103 (2007)
- Wareham DW, "Bloodstream infection due to Acinetobacter spp: epidemiology, risk factors and impact of multi-drug resistance", Eur J Clin Microbiol Infect Dis, 27, pp.607~612 (2008)
- Performance standards for antimicrobial susceptibility testing; eighteenth informational supplement, "Clinical and Laboratory Standards Institute", CLSI, ~, pp.Document M100-S18 (2008)
- Ling TK, "Evaluation of VITEK 2 rapid identification and susceptibility testing system against gram-negative clinical isolates", J Clin Microbiol, 39, pp.2964~2966 (2001)
- Hsieh WS, "Evaluation of the Vitek 2 cards for identification and antimicrobial susceptibility testing of non-glucose-fermenting Gram-negative bacilli", APMIS, 11, pp.241~247 (2009)