Adjustment of Modified Carbapenem Inactivation Method Conditions for Rapid Detection of Carbapenemase-Producing Acinetobacter baumannii
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: The existing modified carbapenem inactivation methods (mCIMs) recommended by the CLSI for detecting carbapenemase production have not been applicable for Acinetobacter baumannii. We evaluated the influence of matrices used in mCIMs and CIMTris on the stability of the disks for detecting carbapenemase producers and suggested optimal mCIM conditions for detecting carbapenemase-producing A. baumannii. Methods: Seventy-three A. baumannii isolates characterized for antimicrobial susceptibility and carbapenemase encoding genes were tested for carbapenemase production using mCIM and CIMTris. The influence of the matrices (Tryptic soy broth [TSB] and Tris-HCl) used in these methods on the stability of the meropenem (MEM) disk was also evaluated. The mCIM conditions were adjusted to enhance screening sensitivity and specificity for detecting carbapenemase-producing A. baumannii. Results: The matrices had an impact on the stability of the MEM disk after the incubation period (two or four hrs). TSB nutrient broth is an appropriate matrix for mCIM compared with Tris-HCl pH 7.6, which leads to the loss of MEM activity in CIMTris. The sensitivity and the specificity of the optimal mCIM were both 100%. Conclusions: We established optimal mCIM conditions for simple, accurate, and reproducible detection of carbapenemase-producing A. baumannii.
Abstract
Background: The existing modified carbapenem inactivation methods (mCIMs) recommended by the CLSI for detecting carbapenemase production have not been applicable for Acinetobacter baumannii. We evaluated the influence of matrices used in mCIMs and CIMTris on the stability of the disks for detecting carbapenemase producers and suggested optimal mCIM conditions for detecting carbapenemase-producing A. baumannii. Methods: Seventy-three A. baumannii isolates characterized for antimicrobial susceptibility and carbapenemase encoding genes were tested for carbapenemase production using mCIM and CIMTris. The influence of the matrices (Tryptic soy broth [TSB] and Tris-HCl) used in these methods on the stability of the meropenem (MEM) disk was also evaluated. The mCIM conditions were adjusted to enhance screening sensitivity and specificity for detecting carbapenemase-producing A. baumannii. Results: The matrices had an impact on the stability of the MEM disk after the incubation period (two or four hrs). TSB nutrient broth is an appropriate matrix for mCIM compared with Tris-HCl pH 7.6, which leads to the loss of MEM activity in CIMTris. The sensitivity and the specificity of the optimal mCIM were both 100%. Conclusions: We established optimal mCIM conditions for simple, accurate, and reproducible detection of carbapenemase-producing A. baumannii.
참고문헌 (17)
- Dijkshoorn L, "An increasing threat in hospitals : multidrug-resistant Acinetobacter baumannii", Nat Rev Microbiol, 5, pp.939~951 (2007)
- Peleg AY, "Acinetobacter baumannii : Emergence of a successful pathogen", Clin Microbiol Rev, 21, pp.538~582 (2008)
- Poirel L, "Carbapenem resistance in Acinetobacter baumannii : mechanisms and epidemiology", Clin Microbiol Infect, 12, pp.826~836 (2006)
- Sunenshine RH, "Multidrug-resistant Acinetobacter infection mortality rate and length of hospitalization", Emerg Infect Dis, 13, pp.97~103 (2007)
- Queenan AM, "Carbapenemases : the versatile β-lactamases", Clin Microbiol Rev, 20, pp.440~458 (2007)
- Pierce VM, "Modified carbapenem inactivation method for phenotypic detection of carbapenemase production among Enterobacteriaceae", J Clin Microbiol, 55, pp.2321~2333 (2017)
- van der Zwaluw K, "The carbapenem inactivation method(CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in Gram-negative rods", PLoS One, 10, pp.e0123690~ (2015)
- Walther-Rasmussen J, "OXA-type carbapenemases", J Antimicrob Chemother, 57, pp.373~383 (2006)
- Simner PJ, "Carbapenemase detection among carbapenem-resistant glucose-nonfermenting Gram-negative bacilli", J Clin Microbiol, 55, pp.2858~2864 (2017)
- CLSI, "Performance standards for antimicrobial susceptibility testing" (2016), Clinical and Laboratory Standards Institute
- CLSI, "Performance standards for antimicrobial susceptibility testing" (2017), Clinical and Laboratory Standards Institute
- CLSI, "Performance standards for antimicrobial susceptibility testing" (2018), Clinical and Laboratory Standards Institute
- CLSI, "Test for carbapenemases in Enterobacteriaceae and Pseudomonas aeruginosa" (2018), Clinical and Laboratory Standards Institute
- Uechi K, "A modified carbapenem inactivation method, CIMTris, for carbapenemase production in Acinetobacter and Pseudomonas species", J Clin Microbiol, 55, pp.3405~3410 (2017)
- CLSI, "Performance standards for antimicrobial susceptibility testing" (2010), Clinical and Laboratory Standards Institute
- Woodford N, "Multiple PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp", Int J Antimicrob Agents, 27, pp.351~353 (2006)
- Bahador A, "The prevalence of ISAba1 and ISAba4 in Acinetobacter baumannii species of different international clone lineages among patients with burning in Tehran, Iran", Jundishapur J Microbiol, 8, pp.e17167~ (2015)