항산균 배양에서 수산화나트륨 세척제로 증류수 사용에 대한 평가
Evaluating the Use of Distilled Water for Washing Sodium Hydroxide in Mycobacterial Culture
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
배경: 항산균 검사를 위한 호흡기 검체는 점액과 상재균 제거를 위해 N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH)로 전처리한 후, 인산완충용액으로 세척과 중화를 거치게 된다. 본 연구에서는 인산완충용액대신에 증류수 사용에 대한 유용성을 평가하고자 하였다. 방법: 2016년 10월부터 2018년 9월까지 2년 동안 광주 소재 종합 병원에서 진행된 항산균검사를분석했다. 호흡기검체의 전처리 세척제로 인산완충용액과 증류수를 각각 1년씩 사용하여 검사하였다. 결과: 배양 양성률은 인산완충용액 사용시기에 12.7% (1,843/14,532), 증류수 사용시기에 14.7% (2,095/14,291)였다. MGIT의 양성률과 MTBC와 NTM의 분리비는 변화가 없었다. 그러나 2% Ogawa media에서는 NTM의 배양이 47.4% (399/841)에서 56.1% (630/1,122)로 증가함에 따라 양성률이 45.6% (841/1,843)에서 53.6% (1,121/2,095)로 증가하였다. MGIT 오염률은 6.5%에서 4.1% 로감소하였다결론: NALC-NaOH 세척제로 증류수를 사용하는 것은 MGIT의 오염률은 감소시키면서 Ogawa media의 양성률을 증가시켰다. 따라서, 항산균 배양 검사에서 전처리 세척제로 인산완충용액대신 증류수 사용도 유용할 것으로 사료된다.
Abstract
Background: Respiratory specimens subjected to mycobacterial detection were initially pretreated with N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) to remove the mucus and normal flora. Next, they were washed and neutralized with phosphate-buffered solution (PBS). The effectiveness of distilled water (DW) compared to PBS as a washing neutralizer during identification of mycobacteria was evaluated in this study. Methods: We analyzed the results of mycobacterial test conducted at a general hospital in Gwangju from October 2016 to September 2018. PBS and DW were used as a respiratory sample washing agent for one year each. Results: The positive culture rate for the culture of mycobacteria was 12.7% (1,843/14,532) and 14.7% (2,095/14,291), when PBS and DW were used, respectively. The recovery rate of the mycobacteria growth indicator tubes (MGIT) and the separation rates of Mycobacterium tuberculosis complex and nontuberculous mycobacteria (NTM) showed no significant change. However, in 2% Ogawa medium, as the NTM culture increased from 47.4% (399/841) to 56.1% (630/1,122), the recovery rate increased from 45.6% (841/1,843) to 53.6% (1,122/2,095). The MGIT contamination rate decreased from 6.5% to 4.1%. Conclusion: DW as a washing agent for NALC-NaOH increased the recovery rate of Ogawa media and reduced the contamination rate of MGIT. Therefore, use of DW instead of PBS as a washing neutralizer during identification of mycobacteria might be useful.
참고문헌 (14)
- 황상선, "LG AdvanSure TB", NTM Real-Time PCR Kit의 항산균 진단 수행 능력 평가, 2011, pp.14(2), 55~59[DOI]
- Kim YS, "Comparison of the MGIT(mycobacteria growth indicator tube)with Ogawa media for recovery of mycobacteria", Korean J Clin Microbiol, 4, pp.58~61 (2001)
- Go UY, "Tuberculosis prevention and care in Korea : evolution of policyand practice", J Clin Tuberc Other Mycobact Dis, 11, pp.28~36 (2018)
- Schlossberg D. Tuberculosis, "6th ed" (2011), DC; ASM Press
- Griffith DE, "and prevention of nontuberculous mycobacterial diseases", Am J Respir Crit Care Med, 175, pp.367~416 (2007)
- 강형석, "객담 전처리 방법에 따른 객담 항산균 도말 및 배양 양성률 비교연구", Tuberculosis and Respiratory Diseases, 65(5), pp.379~384 (2008), 대한결핵및호흡기학회
- Beran V, "Cell wall deficient forms of mycobacteria : a review", Vet Med, 7, pp.365~389 (2006)
- Yi JY, "Detection of Mycobacterium tuberculosis using BACTEC mycobacteria growth indicator tube(MGIT)960 system : comparison with BACTEC 460 TB system and Ogawa media", Korean J Clin Pathol, 20, pp.384~391 (2000)
- Subramanyam B, "Reprocessing of contaminated MGIT 960 cultures to improve availablility of valid results for mycobacteria", Int J Microbiol, 1721020, pp.1~3 (2020)
- 정해용, "마이코박테리아 검출을 위하여 MGIT 양성 신호를 보인 세균에오염된 검체 재처리 과정의 효율성 평가", 대한임상검사과학회지, 51(2), pp.171~176 (2019), 대한임상검사과학회[DOI]
- Krasnow I, "The mortality rate of tubercle bacilli in various digestion systems", Am J Clin Pathol, 45, pp.352~355 (1966)
- 배은신, "항산균 배양에서 BACTEC Mycobacteria Growth Indicator Tube 960 System과 Ogawa 배지 병용의 평가", Annals of Laboratory Medicine, 28(4), pp.299~306 (2008), 대한진단검사의학회
- Joung US, "Comparision of mycobacterial culture by mycobacterium growth indicator tube and Ogawa media", Korean J Clin Microbiol, 7, pp.135~138 (2004)
- Kim YS, "Comparison of the MGIT(mycobacteriia growth indicator tube)with Ogawa media for recovery of mycobacteria", Korean J Clin Microbiol, 4, pp.58~61 (2001)