본문 바로가기
KERD
논문KCI 등재

Detection of Mycobacterium bovis in the lymph node of tuberculin positive cattle by guanidium isothiocyanate/silica DNA extraction and polymerase chain reaction

김종만|유한상|정석찬|조윤상
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2007.06|한국동물위생학회지|제30권 제2호|pp. 233-241

2

조회

0

다운로드

0

인용

0

참고문헌

주제분류수의학

초록

Abstract

Tuberculin positive cattle without gross tubercle lesions should be confirmed by the bacteriological examination to determine the state of the infection. To overcome the time-consuming and laborious identification by culture and biochemical tests, polymerase chain reaction(PCR) has been used to identify Mycobacterium bovis. Due to various lipids in the cell wall of Mycobacterium spp, novel methods of DNA extraction from Mycobacterium spp have been developed. In this study, a newly developed guanidium isothiocyanate/silica DNA extraction method was directly applied to specimens from the tuberculin positive cattle. DNAs were directly extracted from the lymph nodes and the major polymorphic tandem repeat(MPTR) and mycobacterial protein of BCG 70(MPB70) were amplified using PCR. The DNA extraction method using guanidium isothiocyanate/silica was efficient and safe, and the MPTR and MPB70 primers were specific to M bovis. Therefore, MPTR and MPB70 PCRs will be useful for the detection of M bovis in the lymph node from skin-test positive cattle.

참고문헌 (36)

  1. "Rapid differentiation of Mycobacterium bovis and Mycobacterium tuberculosis based on a 12.7-kb fragment by a single tube multiplex-PCR", 109, pp.211~216 (2005)
  2. "Stephens DM. 1991. Identification of an insertion sequence", pp.11~16
  3. "Comparison of C18-carboxypropylbetaine and glass bead DNA extraction methods for detection of Mycobacterium bovis in bovine milk samples and analysis of samples by PCR", 64, pp.3099~3101 (1998)
  4. "Simple and Rapid Detection of Mycobacterium tuberculosis Complex Organisms in Bovine Tissue Samples by PCR", 33, pp.33~36 (1995)
  5. "Direct detection and identification of Mycobacterium tuberculosis and Mycobacterium bovis in bovine samples by a novel nested PCR assay: Correlation with conventional techniques", 43, pp.5670~5678 (2005)
  6. "Patarroyo ME. 1991. Amplification of a speciesspecific DNA fragment of Mycobacterium tuberculosis and its possible use in diagnosis. J Clin Microbiol 29", pp.2163~2168
  7. "A Multiplex approach to molecular detection of Brucella abortus and", or Mycobacterium bovis infection in cattle, 2000, pp.38, 2602~2610
  8. "et al. 1990. Epitope mapping of the Mycobacterium bovis secretory protein MPB70 using over- lapping peptide analysis. J Gen Microbiol 136", pp.265~272
  9. "et al. 1990. Detection and identification of Mycobacterium tuberculosis by DNA amplification. J Clin Microbiol 28" (1877)
  10. "et al. 1990. Insertion element IS986 from Mycobacterium tuber- culosis a useful tool for diagnosis and epidemiology of tuberculosis. J Clin Microbiol 28", pp.2501~2508
  11. "Differentiation of stains in Mycobacterium tuberculosis complex by DNA sequence polymorphisms, including rapid identification of M. bovis BCG", 33, pp.840~844 (1995)
  12. "et al. 1994. Sensitivity and Specificity of PCR for detection of Mycobacterium tuberculosis a blind comparison study among seven laboratories. J Clin Microbiol 32", (ordhoek gt), pp.277~284
  13. "et al. 1986. Properties of proteins MPB64 and MPB80 of Mycobacterium bovis BCG. Infect Immun 52", pp.293~302
  14. "Russell WP. 1993. Processing and secretion by Es- cherichia coli of a recombinant from of the immunogenic protein MPB70 of Mycobacterium bovis. J Gen Microbiol 139", pp.1253~1259
  15. "et al. 1994. Identification of bovine T-cell epitopes for three Myco- bacterium bovis antigens", pp.000 9~15
  16. "et al. 1990. Protein G-based enzyme- linked immunosorbent assay for antiMPB70 antibodies in bovine tuberculosis. J Clin Microbiol 28", pp.913~921
  17. "Richards WD. 1972. Inhibition of fungal growth in the cultural isolation of mycobacteria. Appl Microbiol 24", pp.205~207
  18. "Thurston JR. 1968. Susceptibilities of mycobacterial and nocardial species to benzalkonium chloride. Am J Vet Res 24", pp.759~761
  19. "Jarnagin JL. 1974. Comparison of six methods for isolating mycobacteria from swine lymph nodes. Appl Microbiol 27", pp.448~451
  20. "Trajstman AC. 1988. An evaluation of 1-hexadecylpyridium chloride as a decontaminant in the primary isolation of Mycobacterium bovis from bovine lesions. Vet Microbiol 18", pp.127~134
  21. "et al. 1991. Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. Am Rev Respir Dis 144", pp.1160~1163
  22. "et al. 1991. Purification and characterization of major antigens from a Mycobacterium bovis culture filtrate. Infect Immun 59", pp.800~807
  23. "Multiprimer PCR system for differential identification of Mycobacteria in clinical samples", 34, pp.324~328 (1996)
  24. "et al. 1991. Use of a reamplification protocol improves sensitivity of detection of Mycobacterium tuberculosis in clinical samples by amplification of DNA. J Clin Microbiol 29", pp.712~717
  25. "Identification of Mycobacterium bovis in bovine clinical samples by PCR species-specific primers", 63, pp.101~106 (1999)
  26. "Simultaneous detection and strain differentiation of Mycobacterium bovis directly from bovine tissue specimens by spoligotyping", 74, pp.227~236 (2000)
  27. "Detection of Mycobacterium bovis in tissues by polymerase chain reaction", 43, pp.227~240 (1995)
  28. "et al. 1990. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28", pp.495~503
  29. "et al. 1991. Rapid purification of hepatitis B virus DNA from serum. J Clin Microbiol 29" (1804)
  30. "Lee KP. 1994. Rapid and simple method for extraction of Mycobacteria species DNA for polymerase chain reaction. J Korean Soc Microbiol 29", pp.147~152
  31. "Simple method for treating clinical specimens containing Mycobacterium tuberculosis to remove DNA for polymerase chain reaction. J Clin Microbiol 30", pp.1331~1334
  32. "Nagasuga T. 1981. Specific skin-reactive protein from culture filtrate of Mycobac- terium bovis BCG. Infect Immun 31", pp.1152~1160
  33. "Lee KP. 1994. Amplification of Mycobacterium tuberculosis DNA in blood of patients with granulomatous lesions by polymerase chain reaction. J Korean Soc Microbiol 29", pp.269~274
  34. "Shon BW. 1994. Detection of Mycobacterium bovis in the pathological lesions of slaughtered dairy cattles using modified culture medium and polymerase chain reaction. Kor J Vet Publ Hlth 18"
  35. "et al. 1991. Insertion elements IS987 from Mycobacterium bovis BCG is located in a hotspot integration for insertion elements in Mycobacterium tuberculosis complex strains. Infect Immun 59", pp.2695~2705
  36. "an IS-like element of Mycobacterium tuber- culosis complex. Nucleic Acids Res 18", pp.188~

관련 논문AI추천

PCR 기법을 이용한 도축소의 결핵병 신속진단김용환, 김재익, 김현중, 박덕웅, 박성도, 박종태, 고바라다한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제3호소 림프절에서 Mycobacterium bovis DNA의 신속 검출과 M. bovis와 M. tuberculosis 감별을 위한 real-time PCR 개발구복경, 김용환, 나호명, 문용운, 박성도, 배성열, 장영부, 조호성, 고바라다한국동물위생학회 · 한국동물위생학회지2011 · 제34권 제4호Dual priming oligonucleotide system for the multiplex detection of tuberculosis in Hanwoo김용환, 박남용, 신종봉, 조호성한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제4호한우 결핵의 신속 감별진단을 위한 등온증폭법 개발이태욱, 정대영, 조호성, 황은숙한국동물위생학회 · 한국동물위생학회지2011 · 제34권 제4호DNA 추출없이 전혈을 이용한 PCR-전기영동법에 의한소의 타일레리아병 진단강성호, 김용성, 장상민, 채준석대한화학회 · 대한화학회지2003 · 제47권 제2호Mycobacterium bovis와 M. tuberculosis 감별을 위한 등온증폭법김용환, 김은선, 김재명, 나호명, 박성도, 성창민, 지태경, 고바라다한국동물위생학회 · 한국동물위생학회지2013 · 제36권 제2호

이 간행물의 다른 논문 (12건)

Effectiveness of the Verif EYETM machine-vision technology for complying with reducing microbial indicator counts on beef carcasses김기철, 이양수, 김사현, 장원혁, 오혜원, 정태남, 이정아, 이상관, 정진창pp. 191-196Seroprevalence of swine influenza and porcine reproductive and respiratory syndrome in Korea한정희, 박영일, 정광, 진웬pp. 197-203Development and evaluation of surface plasmon resonance imaging for the detection of antibodies against classical swine fever virus in swine이태욱, 조호성, 박남영pp. 205-209Detection of rotavirus and coronavirus from suckling Korean indigenous calves with acute diarrhea송희종, 이한경, 전승기pp. 211-218Experimental development of caprine enterotoxaemia with Clostridium perfringens type D whole culture in natural host and its treatmentsMd Sidiqur Rahman, MJFA Taimur, Md. Ershaduzzaman, 송희종, KBMS Islampp. 219-231
원문 보기