Mycobacterium bovis와 M. tuberculosis 감별을 위한 등온증폭법
Loop-mediated isothermal amplification assay for differentiation of Mycobacterium bovis and M. tuberculosis
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초록
Mycobacterium (M.) bovis, a member of the M. tuberculosis complex (MTC), is a re-emerging, zoonotic agent of bovine tuberculosis whose prevalence probably depends on variations in direct exposure to cattle and ingestion of raw milk. Accurate species differentiation of M. bovis and M. tuberculosis is needed to distinguish between human and zoonotic tuberculosis. This study successfully developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection and differentiation of M. bovis and M. tuberculosis, however showed negative reactions in eight non-tuberculous mycobacteria (NTM) samples and ten other bacterial species. Sensitivity of this assay for detection of genomic M. bovis DNA was 10 fg/ml. And this assay successfully detected M. bovis in bovine clinical specimens. In conclusion, the LAMP assay is a simple and powerful tool for rapid detection of M. bovis in both pure bacterial culture and in clinical samples.
Abstract
Mycobacterium (M.) bovis, a member of the M. tuberculosis complex (MTC), is a re-emerging, zoonotic agent of bovine tuberculosis whose prevalence probably depends on variations in direct exposure to cattle and ingestion of raw milk. Accurate species differentiation of M. bovis and M. tuberculosis is needed to distinguish between human and zoonotic tuberculosis. This study successfully developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection and differentiation of M. bovis and M. tuberculosis, however showed negative reactions in eight non-tuberculous mycobacteria (NTM) samples and ten other bacterial species. Sensitivity of this assay for detection of genomic M. bovis DNA was 10 fg/ml. And this assay successfully detected M. bovis in bovine clinical specimens. In conclusion, the LAMP assay is a simple and powerful tool for rapid detection of M. bovis in both pure bacterial culture and in clinical samples.
참고문헌 (29)
- Ahmed MU, "Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensor", Food Control, 21, pp.599~605 (2010)
- Aryan E, "A novel and more sensitive loop-mediated isothermal amplification assay targeting IS6110 for detection of Mycobacterium tuberculosis complex", Microbiol Res, 165, pp.211~220 (2010)
- Bi A, "A rapid loop-mediated isothermal amplification assay targeting hspX for the detection of Mycobacterium tuberculosis complex", Jpn J Infect Dis, 65, pp.247~251 (2012)
- Gandelman O, "Loop-mediated amplification accelerated by stem primers", Int J Mol Sci, 12, pp.9108~9124 (2011)
- Gandelman OA, "Novel bioluminescent quantitative detection of nucleic acid amplification in real-time", PLoS ONE, 5, pp.e14155~ (2010)
- Geojith G, "Efficacy of loop mediated isothermal amplification (LAMP) assay for the laboratory identification of Mycobacterium tuberculosis isolates in a resource limited setting", J Microbiol Methods, 84, pp.71~73 (2011)
- Grange JM, "Bovine tubercle bacilli and disease in animals and man", Epidemiol Infect, 99, pp.221~234 (1987)
- Hong M, "A modified visual loop-mediated isothermal amplification method for diagnosis and differentiation of main pathogens from Mycobacterium tuberculosis complex", World J Microbiol Biotechnol, 28, pp.523~531 (2012)
- 황은숙, "한우 결핵의 신속 감별진단을 위한 등온증폭법 개발", 한국가축위생학회지, 34(4), pp.333~339 (2011), 한국가축위생학회
- Iwamoto T, "Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples", J Clin Microbiol, 41, pp.2616~2622 (2003)
- Jung C, "Isothermal target and signaling probe amplification method, based on a combination of an isothermal chain amplification technique and a fluorescence resonance energy transfer cycling probe technology", Anal Chem, 82, pp.5937~5943 (2010)
- 고바라다, "소 림프절에서 Mycobacterium bovis DNA의 신속 검출과 M. bovis와 M. tuberculosis 감별을 위한 real-time PCR 개발", 한국가축위생학회지, 34(4), pp.321~331 (2011), 한국가축위생학회
- Liang C, "Multiplex loop-mediated isothermal amplification detection by sequence-based barcodes coupled with nicking endonuclease-mediated pyrosequencing", Anal Chem, 84, pp.3758~3763 (2012)
- 임성경, "국소적 농흉으로 나타난 Mycobacterium bovis 감염 1예", 대한내과학회지, 81(6), pp.792~796 (2011), 대한내과학회
- Mori Y, "Sequence specific visual detection of LAMP reactions by addition of cationic polymers", BMC Biotechnol, 6, pp.3~ (2006)
- Mori Y, "Real-time turbidimetry of LAMP reaction for quantifying template DNA", J Biochem Biophys Methods, 59, pp.145~157 (2004)
- Mori Y, "Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation", Biochem Biophys Res Commun, 289, pp.150~154 (2001)
- Nagamine K, "Accelerated reaction by loop-mediated isothermal amplification using loop primers", Mol Cell Probes, 16, pp.223~229 (2002)
- Nagamine K, "Isolation of single- stranded DNA from loop-mediated isothermal amplification products", Biochem Biophys Res Commun, 290, pp.1195~1198 (2002)
- Nagamine K, "Loop-mediated isothermal amplification reaction using a nondenatured template", Clin Chem, 47, pp.1742~1743 (2001)
- Notomi T, "Loop-mediated isothermal amplification of DNA", Nucleic Acids Res, 28, pp.E63~ (2000)
- Pandey BD, "Development of an in-house loop-mediated isothermal amplification (LAMP) assay for detection of Mycobacterium tuberculosis and evaluation in sputum samples of Nepalese patients", J Med Microbiol, 57, pp.439~443 (2008)
- Parra A, "Development of a molecular diagnostic test applied to experimental abattoir surveillance on bovine tuberculosis", Vet Microbiol, 127, pp.315~324 (2008)
- Thoen C, "The importance of Mycobacterium bovis as a zoonosis", Vet Microbiol, 112, pp.339~345 (2006)
- Tomita N, "Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products", Nat Protoc, 3, pp.877~882 (2008)
- Tong Y, "Development of isothermal TaqMan assays for detection of biothreat organisms", Biotechniques, 45, pp.543~557 (2008)
- Zhang J, "Development of a loop-mediated isothermal amplification assay for the detection of Mycobacterium bovis", Vet J, 187, pp.393~396 (2011)
- Zhu RY, "Use of visual loop-mediated isothermal amplification of rimM sequence for rapid detection of Mycobacterium tuberculosis and Mycobacterium bovis", J Microbiol Methods, 78, pp.339~343 (2009)
- Zumárraga M, "A 12.7 kb fragment of the Mycobacterium tuberculosis genome is not present in Mycobacterium bovis", Microbiology, 145, pp.893~897 (1999)
