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PCR 기법을 이용한 도축소의 결핵병 신속진단

Rapid diagnosis of bovine tuberculosis in slaughter cattle using PCR

김용환|김현중|박종태|김재익|고바라다|박성도|박덕웅
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2007.09|한국동물위생학회지|제30권 제3호|pp. 393-406

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참고문헌

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초록

Abstract

Bovine tuberculosis is an important zoonosis worldwide. Mycobacterium bovis, the causative agent of this disease in cattle, is also a pathogen for humans and several economically important animals. The cases of tuberculosis are reported in two cow found at slaughter house located in Gwangju city. Histopathologically, in the lymph nodes, granulomas consisted of large areas of necrosis surrounded by variable thick bands of cellular infiltrate containing macrophages, Langhanstype multinucleated giant cells and lymphocytes. Lesions in the lung followed the same developmental pattern as did lesions in the lymph nodes with some exceptions. With the acidfast staining, numerous mycobacteria were revealed in the lung and lymph nodes. M bovis was confirmed as a causative agent in these cattle using bacterial isolation and PCR and restriction fragment length polymorphism method based on a unique 12.7kb fragment insertion sequence from the Mycobacterium tuberculosis genome and the pncA polymorphism. The insertion element IS6110 and IS1081 were present M bovis isolated from lungs and lymph nodes of cattle using PCR assay. *Corresponding authorPhone : +82-62-571-0498, Fax : +82-62-571-0496E-mail: barada@hanmail.net These cases are interesting and important in public health aspect that M bovisinfected cattle were found during a routine postmortem inspection at slaughter house.

참고문헌 (45)

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