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Dual priming oligonucleotide system for the multiplex detection of tuberculosis in Hanwoo

김용환|박남용|조호성|신종봉
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2007.12|한국동물위생학회지|제30권 제4호|pp. 527-532

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Abstract

In present study, we described the reliability of the dual priming oligonucleotide(DPO) multiplex polymerase chain reaction(PCR) for the detection of Mycobacterium tuberculosis complex(MTC) and non-Mycobacterium tuberculosis(NMT) in blood samples of the Korea native cattle, Hanwoo. Among 340 samples 22(6.5%) were positive in using DPO multiplex PCR, 21(6.2%) were positive in PCR. The relative agreement between 2 tests was 99.7%, and the agreement quotient(kappa), was 0.95(excellent). In these results, we demonstrated the successful application of DPO multiplex PCR for the diagnosis of bovine tuberculosis in Hanwoo. Multiplex PCR, using DPO primers, can be useful for the simple diagnosis of bovine tuberculosis in bovine blood samples.

참고문헌 (16)

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관련 논문AI추천

PCR 기법을 이용한 도축소의 결핵병 신속진단김용환, 김재익, 김현중, 박덕웅, 박성도, 박종태, 고바라다한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제3호한우 결핵의 신속 감별진단을 위한 등온증폭법 개발이태욱, 정대영, 조호성, 황은숙한국동물위생학회 · 한국동물위생학회지2011 · 제34권 제4호소 림프절에서 Mycobacterium bovis DNA의 신속 검출과 M. bovis와 M. tuberculosis 감별을 위한 real-time PCR 개발구복경, 김용환, 나호명, 문용운, 박성도, 배성열, 장영부, 조호성, 고바라다한국동물위생학회 · 한국동물위생학회지2011 · 제34권 제4호Mycobacterium bovis와 M. tuberculosis 감별을 위한 등온증폭법김용환, 김은선, 김재명, 나호명, 박성도, 성창민, 지태경, 고바라다한국동물위생학회 · 한국동물위생학회지2013 · 제36권 제2호Detection of Mycobacterium bovis in the lymph node of tuberculin positive cattle by guanidium isothiocyanate/silica DNA extraction and polymerase chain reaction김종만, 유한상, 정석찬, 조윤상한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제2호도축한우에서 발견된 결핵병곽학구, 변현섭, 안병우, 이상명, 이현주, 조윤상, 최해연, 한성태한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제3호

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