Application of the melanocortin 1 receptor(MC1R) gene for discrimination of Hanwoo from Holstein beef using real？time polymerase chain reaction(PCR)
2
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
This study was carried out to discriminate Hanwoo from the milking and hybrid cattle by detection of MC1R gene related to bovine hair color. One hundred sixty six samples were collected from the abattoir(n=106) and local market(n=60). The beef from abattoir were originated from Hanwoo(n=27), Holstein(n=29), Hybrid(n=45) and imported cattle(n=5), respectively. The beef from market consisted of Hanwoo(n=36), Holstein(n=7) and imported ones(n=17). Commercialized screening kit (Kogenebiotec, Korea) was used for MC1R gene analysis. As a result, Hanwoo was discriminated from Holstein. However, 9 of 45 hybrid and 11 of 22 imported beef samples were indistinguishable from Hanwoo. It could be explained by second generation of crossing of Hanwoo with Holstein or the cattle with silver or yellow hair. This results suggest that additional tests as well as MC1R gene detection be needed to confirm Hanwoo beef among cattle beef._____________________________
참고문헌 (7)
- Chung ER, "2000. Identification of Hanwoo meat using PCR-RFLP marker of MC1R gene associated with bovine coat color", 42(4), pp.379~390 (2000)
- Park SD, "Identification of Hanwoo and Holstein meat using MGB probe based real?time PCR associated with single nucleo?tide polymorphism(SNP) in Melanocortin 1 receptor(MC1R) gene", 45(1), pp.25~28 (2005)
- Kim TJ, "Discrimination of Hanwoo from Holstein", black Angus meat by PCR-RFLP of MC1R gene, 2005, pp.45(3), 335~339
- Koh BRD, "Rapid differentiation of Hanwoo and Holstein meat using multiplex allele specific polymerase chain reaction protocols", 45(3), pp.351~357 (2005)
- Chung ER, "Identification of Hanwoo Meat and Analysis of Polymorphism of Bovine MC1R Gene using PCR-SSCP Technique", 43(1), pp.45~52 (2001)
- McPeake SR, "Color pattern inheritance in beef cattle" (2004), University of Arkansas Division of Agriculture Cooperative Extension Service, Little Rock
- Novelfood Team of Nutritional, "Method for Identification of cow meat origin to differentiate Korean cow meat from imported cow meat" (2006), Korea Food^Drug Administration