칡소와 비경흑색 한우의 Melanocortin Receptor 1 (MC1R)유전자형 분석

Abstract

PCR-RFLP analysis was carried out to investigate the genotype of Melanocortin receptor 1 (MC1R) gene in Korean Brindle Cattle and Korean Cattle with dark muzzle, which are coat color and muzzle pigmentation variants of Korean Cattle, respectively. Allelic variants of MC1R in cattle were analyzed by digestion with BsrFⅠ, AciⅠ. Among six genotypes, ED/ED, ED/E+, ED/e, E+/E+, E+/e and e/e, detected in cattle, only two genotypes, E+/E+ and E+/e, were observed in Korean Brindle Cattle, probably reflecting the necessary of E+ allele for the expression of black brindle coat color. As in Korean Cattle with light muzzle, the E+/e and e/e genotypes were detected in Korean Cattle with dark muzzle. The E+ and e alleles frequencies in two populations of Korean Cattle with dark muzzle and with light muzzle were 0.37, 0.63 and 0,11, 0.89, respectively. Although the frequency of E+ allele in Korean Cattle with dark muzzle was higher than in Korean Cattle with light muzzle, the E+ allele was not completely associated with dark muzzle pigmentation. The results of this experiment indicate that the difference of MC1R genotype and frequency may be useful for fixation of coat color in Korean Cattle as well as Korean Brindle Cattle.