포유자돈에서 돼지써코바이러스 2형이 돼지유행성설사 바이러스 감염에 미치는 영향 Ⅱ. 임상증상, 병리조직학적 검사 및 면역조직학적 검사
Effects of porcine circovirus type 2 on the pathogenesis of porcine epidemic diarrhoea virus infection in piglets Ⅱ. Clinical sign, histopathological lesion and immunohistochemical finding
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Abstract
The purpose of this study was to evaluate the effect of a subsequent infection of PCV2 on piglets with PEDV. In clinical signs, the signs observed in dual infected with PEDV and PCV2 piglets and alone infected with PEDV piglets ranged from diarrhoea to vomiting and dehydration. Dual infected piglets developed signs of anorexia, vomiting and watery diarrhoea within 12 hpi. Nevertheless alone infected piglets caused pasty diarrhea at first. In mortality, dual infections showed 25%, but alone infections showed 8.3％, respectively. In gross findings, piglets of dual infections group appeared the severe findings of congestion, distension of lumen, contaning curdes of undigested milk in stomach milder than those of alone infections group. In histopathological findings, piglets of dual infection group appeared the more severe findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. Significant (P⁄0.05) decrease in VH : CD ratio in dual infected piglets compared to piglets from alone PEDV infections. In immunohistochemical findings, strong hybridization signals in dual infections were seen in areas of moderate to severe villous atrophy or vacuolation with positive cells arranged continuously over the villi. In the lumen, exfoliated enterocytes were strongly positive in dual infected piglets. The number of PEDV-positive cells in dual infected pigs was significantly higher than that in alone PEDV-infected piglets.
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