돼지 써코바이러스 2형 국내분리주의 유전학적 특성 규명
Genetic characterization and phylogenetic analysis of porcine circovirus type 2 field strains isolated from Korean pocine circovirus disease (PCVD) pigs
1
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
In order to obtain the genetic information of the Korean isolates of porcine circovirus 2 (PCV2), complete genomes of five isolates from Korean PCVD weaned pigs with wasting syndromes were sequenced and compared with those of other published PCV2 isolates. Of the five PCV2 isolates, four (1767 nucleotides) were classified into PCV2b, and one (1,768 nucleotides) was PCV2a. Moreover, it appeared that PCV2b is now the dominant genotype circulating in Korea herds. Total complete genomes of four PCV2b isolates shared 99.1~99.4% nucleotide sequence homology each other, and were only 95.4~96.2% similar to one PCV2a isolate. ORF2 genome of four PCV2b isolates shared over 99% nucleotide sequence and deduced amino acid sequence identity to each other. Nevertheless, those were much divergent with the PCV2a isolate of this study and ranged from 92.3~92.7% nucleotide homology and 91.9~92.3% deduced amino acid sequence homology, respectively. The amino acid sequence alignments of the putative capsid protein identified three major regions of amino acid heterogeneity at residues 59~91, 121-136 and 190~210. Two of those correspond with dominant immunoreactive areas. Phylogenetic analysis based on the complete genome of PCV2 isolates showed that four PCV2b isolates of this study existed the closest relationship with European strains (Netherland, UK and France). One PCV2a isolate was closely related to Japan and North America strains.
참고문헌 (25)
- 박최규, "Porcine circovirus2 국내 분리주의 유전적 특성", 대한수의학회지, 44(4), pp.571~579 (2004), 대한수의학회
- Allan G, "PCV2: Ticking time bomb", Pig Progress, 18, pp.14~15 (2002)
- Allan GM, "Porcine circoviruses: a review", J Vet Diagn Invest, 12(1), pp.3~14 (2000)
- Allan GM, "Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe", J Vet Diagn Invest, 10, pp.3~10 (1998)
- Allan GM, "Isolation and characterization of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland", Vet Microbiol, 66, pp.115~123 (1999)
- An DJ, "Phylogenetic characterization of porcine circovirus type 2 in PMWS and PDNS Korean pigs between 1999 and 2006", Virus Res, 129, pp.115~12 (2007)
- Boisseson C, "Molecular characterization of porcine circovirus type 2 isolates from post-weaning multisystemic wasting syndrome-affected and non-affected pigs", J Gen Virol, 85, pp.293~304 (2004)
- Carman S, "Porcine circovirus-2 associated disease in swine in Ontario (2004 to 2005)", Can Vet J, 47, pp.761~762 (2006)
- Cheung AK, "Detection of two porcine circovirus type 2 genotypic groups in United States swine herds", Arch Virol, 152, pp.1035~1044 (2007)
- Cheung AK, "Transcriptional analysis of pocine circovirus", J Virol, 305, pp.168~180 (2003)
- DeLay J, "Porcine circovirus type 2-associated disease is increasing", AHL Newsl, 9, pp.22~ (2005)
- Fenaux M, "Genetic characterization of type 2 pocine circovirus (PCV-2) from pigs with post-weaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2", J Clin Microbiol, 38, pp.2494~2503 (2000)
- Gagnon CA, "The emergence of porcine circovirus 2b genotype (PCV-2b) in swine in Canada", Can Vet J, 48, pp.811~819 (2007)
- Grierson SS, "Genome sequence analysis of 10 Dutch isolates from a PMWS case-control study", Res Vet Sci, 77, pp.265~268 (2004)
- Hamel AL, "Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs", J Virol, 72(6), pp.5262~5267 (1998)
- Horlen KP, "A cluster of farms experiencing severe porcine circovirus associated disease: clinical features and association with the PCV2b genotype", J Swine Health Prod, 15, pp.270~278 (2007)
- Young S. Lyoo, "Genetic characterization of porcine circovirus-2 field isolates from PMWS pigs", Journal of Veterinary Science, 3(1), pp.31~39 (2002), 대한수의학회
- Ma CM, "Evidence for recombination in natural populations of porcine circovirus type 2 in Hong Kong and mainland China", J Gen Virol, 88, pp.17~173 (2007)
- Mahe D, "Differential recognition of ORF2 protein from type 1 and type 2 procine circoviruses and identification of immnorelevant epitopes", J Gen Virol, 81, pp.1815~1824 (2000)
- Mankertz A, "Mapping and characterization of the origin of the replication of porcine circovirus", J Virol, 71, pp.2562~2566 (1997)
- McNulty M, "Circoviridae. In:van Regenmortel MHV, Fauquet CM, Bishop DHL, et al, eds. Seventh Report of the International Committee on Taxonomy of Viruses", pp.299~303 (2000), Academic Press
- Olvera A, "Molecular evolution of porcine circovirus type 2 genomes: phylogeny and clonality", Virology, 357, pp.175~185 (2007)
- Opriessnig T, "Genetic and experimental comparison of porcine circovirus type 2 (PCV2) isolates from cases with and without PCV2-assocated lesions provides evidence for differences in virulence", J Gen Virol, 87, pp.2923~2932 (2006)
- Rosell C, "Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome", Vet Rec, 146, pp.40~43 (2000)
- West KH, "Mycocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2", J Vet Diagn Invest, 11, pp.530~532 (1999)