육계에서 깃털을 이용한 플루오르퀴놀론계 잔류 모니터링 가능성 조사
Feasibility of monitoring of fluoroquinolones residual through feather analysis in broilers
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초록
This study was conducted to analyze feasibility of monitoring of fluoroquinolnes residual through feather analysis in broilers. The calibration curve showed good linearity (r 2≥0.99) within the concentration range of 1∼100 μg/kg. The limit of detection (LOD) and limit of quantification (LOQ) were validated at ≤0.66 and ≤1.99 μg/kg in broiler feather, respectively. The recoveries in feather samples ranged from 94.6 to 114.4% (5.1-15.8% RSD) at the 5 to 20 μg/kg spiking levels. The proposed new analytical method proved to be suitable and effective for fluoroquinolnes determination. We also monitored fluoroquinolones residue in 36 samples (broiler that were slaughtered in Gyonggi-do) using this method. Among tested feather samples, enrofloxacin and ciprofloxacin were detected in all samples. In muscle samples, enrofloxacin was detected in 20 (55.6%) samples and ciprofloxacin was not detected.
Abstract
This study was conducted to analyze feasibility of monitoring of fluoroquinolnes residual through feather analysis in broilers. The calibration curve showed good linearity (r 2≥0.99) within the concentration range of 1∼100 μg/kg. The limit of detection (LOD) and limit of quantification (LOQ) were validated at ≤0.66 and ≤1.99 μg/kg in broiler feather, respectively. The recoveries in feather samples ranged from 94.6 to 114.4% (5.1-15.8% RSD) at the 5 to 20 μg/kg spiking levels. The proposed new analytical method proved to be suitable and effective for fluoroquinolnes determination. We also monitored fluoroquinolones residue in 36 samples (broiler that were slaughtered in Gyonggi-do) using this method. Among tested feather samples, enrofloxacin and ciprofloxacin were detected in all samples. In muscle samples, enrofloxacin was detected in 20 (55.6%) samples and ciprofloxacin was not detected.
참고문헌 (24)
- 2012년도 축산 항생제내성균 감시체계 구축 보고서, "농림축산검역본부", 5~30 (2013)
- 닭 및 닭고기 유래 플루오로퀴놀론계 항균제 내성 세균에 대한 내성 저감 및 관리 기술 개발연구 결과보고서, "농림축산검역본부", ~ (2017)
- 서계원, "계란 중 Fluoroquinolone계 합성항균제의 잔류에 관한 연구", 박사, 수의학, pp.2000
- 식품의약품안전처, "식품의 기준 및 규격", 식품의약품안전처 고시 2020-3호, ~ (2020)
- Berendsen B, "The disposition of oxytetracycline to feathers after poultry treatment", Food Addit Contam, 30, pp.2102~2107 (2013)
- Cornejo J, "Determination of oxytetracycline and 4-epi-oxytetracycline residues in feathers and edible tissues of broiler chickens using liquid chromatography coupled with tandem mass spectrometry", J Food Prot, 80, pp.619~625 (2017)
- Cornejoa J, "Depletion of tylosin residues in feathers, muscle and liver from broiler chickens after completion of antimicrobial therapy", Food Addit Contam, 35, pp.448~457 (2018)
- Engberg J, "Quinolone resistant Campylobacter infections : risk factorsand clinical consequences", Emerging Infectious Disease, 10, pp.1056~1063 (2004)
- "Commissioner of Food and Drugs, Docket No. 2000N-1571, Enrofloxacin for Poultry; Final Decision on Withdrawal of New Animal Drug Application Following Formal Evidentiary Public Hearing; Availability" (2005)[URL]
- Gigosos PG, "Determination of quinolones in animal tissues and eggs by high-performance liquid chromatography with photodiode-array detection", J Chromatogr A, 871, pp.31~36 (2000)
- Haag G, "Quantification of residual enrofloxacin and ciprofloxacin in feathers of broiler chickens by high-performance liquid cromatography-fluorescence after oral administration of the drugs", J Adv Pharm Technol Res, 7, pp.2~5 (2016)
- Kim JM, "Antimicrobial drug susceptibility and treatment efficacy in mice against Escherichia coli and Salmonella spp isolated from feces of diarrheal animals", Korea J Vet Res, 37, pp.389~403 (1997)
- Larissa J, "The analysis of tetracyclines, quinolones, macrolides, lincosamides, pleuromutilins, and sulfonamides in chicken feathers using UHPLC-MS", MS in order to monitor antibiotic use in the poultry sector, 2017, pp.409, 4927~4941
- 이영주, "Fluoroquinolone Resistance and gyrA and parC Mutations of Escherichia coli Isolated from Chicken", The Journal of Microbiology, 43(5), pp.391~397 (2005), 한국미생물학회
- 이영주, "닭 분변유래 E.coli 및 Salmonella spp.의 항생제 내성패턴", 대한수의학회지, 45(1), pp.75~83 (2005), 대한수의학회
- López-Cadenas C, "Enrofloxacin : Pharmacokinetics and metabolism in domestic animal species", Curr Drug Metab, 14, pp.1042~1058 (2013)
- María Laura M, "Enrofloxacin and ciprofloxacin Residues in broiler chicken feathers after enrofloxacin oral administration", EC Veterinary Science, 4(3), pp.180~186 (2019)
- Nelson JM, "Fluoroquinolone-resistant Campylobacter species and the withdrawal of fluoroquinolones from use in poultry : a public thealth success story", Clinical Infectious Disease, 44, pp.977~980 (2007)
- Sunjin Park, "The development and validation of a novel liquid chromatography tandem mass spectrometry(LC-MS", MS) procedure for the determination of fluoroquinolones residues in chicken muscle using modified QuEChERS (quick, easy, cheap, effective, rugged and safe) method, 2019, pp.42(4), 289~296[DOI]
- Pokrant E, "Determination of sulfachloropyridazine residue levels in feathers from broiler chickens after oral administration using liquid chromatography coupled to tandem mass spectrometry", PLoS ONE, 13, pp.e0200206~ (2018)
- San Martín B, "Depletion study of enrofloxacin and its metabolite ciprofloxacin in edible tissues and feathers of white leghorn hens by liquid chromatography coupled with tandem mass spectrometry", J Food Protect, 70, pp.1952~1957 (2007)
- Seo KW, "Matrix Solid-Phase Dispersion(MSPD)for the isolation and liquid chromatographic determination of fluoroquinolones in eggs", Korean J Publ Hlth, 26, pp.269~281 (2002)
- Slana M, "Enrofloxacin degradation in broiler chicken manure under field conditions and its residuals effects to the environment", Environmental Science and Pollution Research International, 24, pp.13722~13731 (2017)
- 성명숙, "가금 유래 병원성 대장균의 항균제 내성 및 R plasmid 전달 양상", 대한수의학회지, 48(3), pp.275~285 (2008), 대한수의학회