세종지역 유통 식육의 플루오로퀴놀론계 항생제 잔류 연구
Monitoring for fluoroquinolones residues in raw meat in Sejong
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초록
This study describes an analytical method based on LC-MS/MS for the quantitation of 5 fluoroqui-nolones (Enrofloxacin, Ciprofloxacin, Marbofloxacin, Norfloxacin and Danofloxacin) in meat, and was applied to 230 meat samples for validation. Quantitation was performed based on a matrix-matched calibration to compensate for the matrix effect on the electrospray ionization. Good linear calibrations (R 2≥0.998) were obtained for all fluoroquinolones at 6 concentrations of 1∼50 μg/kg. Satisfied recoveries of all fluoroquinolones were demonstrated in spiked meat at three levels from 10 to 50 μg/kg. The recoveries ranged between 75.8∼99.2% in beef, 80.1∼99.6% in pork and 72.2∼ 99.8% in chicken, respectively. The limits of quantitation (LOQs) for fluoroquinolones ranged from 0.7 to 3.2 μg/kg. We also monitored fluoroquinolones residue in the sample (beef 107, pork 71, chicken 52) using LC-MS/MS. Residues of fluoroquinolones which exceeded maximum residue lim-its (MRL) were not exceed in any of the 230 samples.
Abstract
This study describes an analytical method based on LC-MS/MS for the quantitation of 5 fluoroqui-nolones (Enrofloxacin, Ciprofloxacin, Marbofloxacin, Norfloxacin and Danofloxacin) in meat, and was applied to 230 meat samples for validation. Quantitation was performed based on a matrix-matched calibration to compensate for the matrix effect on the electrospray ionization. Good linear calibrations (R 2≥0.998) were obtained for all fluoroquinolones at 6 concentrations of 1∼50 μg/kg. Satisfied recoveries of all fluoroquinolones were demonstrated in spiked meat at three levels from 10 to 50 μg/kg. The recoveries ranged between 75.8∼99.2% in beef, 80.1∼99.6% in pork and 72.2∼ 99.8% in chicken, respectively. The limits of quantitation (LOQs) for fluoroquinolones ranged from 0.7 to 3.2 μg/kg. We also monitored fluoroquinolones residue in the sample (beef 107, pork 71, chicken 52) using LC-MS/MS. Residues of fluoroquinolones which exceeded maximum residue lim-its (MRL) were not exceed in any of the 230 samples.
참고문헌 (26)
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