원유 세균수 검사장비의 정도관리를 위한 표준시료의 개발 및 평가
Development and evaluation of standard samples for quality control of automated total bacterial counter in raw milk
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Standard samples were prepared for this study, and applied for BactoScan TM and BactoCount TM in order for quality control evaluation for total bacterial count in raw milk. Accordingly, the preparation of two lots of standard samples for quality control were lyophilized, which contain Lactobacillus lactis. The standard samples were prepared into three different levels of bacterial counts, those were Low 30,000∼ 40,000, Medium 70,000∼90,000, High 150,000∼220,000 CFU/mL, respectively. Then, the proficiency tests were performed in total 19 laboratories for measuring total bacterial counts. The total bacterial counts in the standard samples showed 37∼42, 82∼105, 214∼240 CFU/mL in the first lot, and it showed 30∼36, 67∼75, 131∼163 CFU/mL in the second lot in low, medium and high levels, respectively. Based on these results, the absolute values of z-scores of six standard samples in 18 laboratories were ≤2, which means the samples are satisfactory. However, z-score in one laboratory was ≤3, which means the sample is questionable. Using two standard samples, the correlation between BactoScan TM and BactoCount TM was 0.9982, which means the results of total bacterial count measurement of both equipment were equivalent. Therefore, the standard samples manufactured in this study for quality control of total bacterial count using BactoScan TM and BactoCount TM in the raw milk could be applied to proficiency tests.
Abstract
Standard samples were prepared for this study, and applied for BactoScan TM and BactoCount TM in order for quality control evaluation for total bacterial count in raw milk. Accordingly, the preparation of two lots of standard samples for quality control were lyophilized, which contain Lactobacillus lactis. The standard samples were prepared into three different levels of bacterial counts, those were Low 30,000∼ 40,000, Medium 70,000∼90,000, High 150,000∼220,000 CFU/mL, respectively. Then, the proficiency tests were performed in total 19 laboratories for measuring total bacterial counts. The total bacterial counts in the standard samples showed 37∼42, 82∼105, 214∼240 CFU/mL in the first lot, and it showed 30∼36, 67∼75, 131∼163 CFU/mL in the second lot in low, medium and high levels, respectively. Based on these results, the absolute values of z-scores of six standard samples in 18 laboratories were ≤2, which means the samples are satisfactory. However, z-score in one laboratory was ≤3, which means the sample is questionable. Using two standard samples, the correlation between BactoScan TM and BactoCount TM was 0.9982, which means the results of total bacterial count measurement of both equipment were equivalent. Therefore, the standard samples manufactured in this study for quality control of total bacterial count using BactoScan TM and BactoCount TM in the raw milk could be applied to proficiency tests.
참고문헌 (16)
- 공정시험법 세균항목의 정도관리 방안, "국립환경과학원", 49~ (2004)
- 원유검사 표준화 운영 실무 가이드라인, "농림축산검역본부", 43~52 (2019)
- 농림축산식품부, "낙농통계연감", pp.261~ (2016)
- 농림축산식품부, "원유검사 공영화 실시요령", 농림축산식품부 고시 제2016-146호, ~ (2016)
- Bolzoni G, "BactoScan FC : conversion system for results at the national level in Italy and reproducibility of total bacterial counting four years after implementation", Euroreference, 2, pp.46~54 (2017)
- Boucher SN, "Production and viablity of coccoid forms of Campylobacter jejuni", J Appl Bacteriol, 77, pp.303~307 (1994)
- Bulletin of the IDF, "Milk payment systems for ex-farm milk. No 3331", 1998, 1998
- NATHALIE DESMASURES, "Monitoring the microbiology of high quality milk by monthly sampling over 2 years", Journal of Dairy Research, 64(2), pp.271~280 (1997), Cambridge University Press (CUP)[DOI]
- Gunasekera TS, "A flow cytometry method for rapid detection and enumeration of total bacterial bacteria in milk", App Env Microbiol, 66, pp.1228~1232 (2000)[DOI]
- 김주영, "총대장균군 측정의 정도관리에 적합한 균주의 조제", 미생물학회지, 44(1), pp.43~48 (2008), 한국미생물학회
- Madden RH, "Determination of the BactoScan conversion Factor for the United Kingdom", Dairy microbiology, 70, pp.15~17 (2017)
- "REGULATION (EC) No 853", 2004 of THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 29 April 2004 laying down specific hygiene rules for the hygiene of foodstuffs
- Rollins DM, "Viable but nonculturable stage of Campylobacter jejuni. and its role in survival in the natural aquatic environment", Appl Environ Microbiol., 52, pp.531~538 (1986)
- Suhren G, "First experiences with automatic flow cytometric determination of total bacterial count in raw milk", Int Dairy Fed Bull, 50, pp.249~275 (2000)
- Walter S, "Application of a unified European conversion factor for the transformation of Bactoscan FC values", Food Agroscope Science, 36, pp.1~11 (2016)