인천광역시에서 유통되는 계란의 플루오로퀴놀론계 항균물질 잔류 실태 조사
A study on the residues of fluoroquinolones in hen's eggs consumed in Incheon Metropolitan city
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This study was carried out to investigate the residue level of fluoroquinolones in hen's general eggs and specific eggs by microbiological assay method and high performance liquid chromatography (HPLC) method. HPLC separation was carried out by reversed phase chromatography on a Symmetry C18 (250 × 4.6 ㎜, 5 ㎛ particle size) with a phase composed of distilled water (containing 0.4% triethylamine and phosphoric acid) : Methanol (780 : 220, v/v), pumped isocratically at a flow rate of 1.0 ㎖/min. A fluorescence detector was utilized with an excitation wavelength of 278 ㎚ and an emission wavelength of 456 ㎚. The calibration curves were linear (γ2≥0.999) over a concentration range of 0.025～0.4 ㎍/㎖. Average recoveries of the five fluoroquinolones in whole eggs at fortified levels of 0.05～0.2 ㎍/g were ranged mean 78.1～91.7% and low coefficient of variation was less than 10% for all analysed samples. The limits of detection and limits of quantification for whole eggs were 1.2～6.0 ng/g and 2.3～9.1 ng/g, respectively. Only one hen's general eggfrom chicken farm in Incheon was detected with the residual fluoroquinolones (Microbiological assay method ; 1 of 47 general eggs) ; the range of residual concentration enrofloxacin was 0.12 ppm. Those in food stores were detected with the residual fluoroquinolones (Microbiological assay method ; 4 of 88 general eggs) ; the ranges of residual concentration enrofloxacin were 0.15～2.2 ppm, ciprofloxacin 0.01～0.06 ppm, and hen's specific eggs (40) in food stores were not detected. For the microbiological assay method of fluoroquinolones in hen's eggs, as the results of comparative analysis, the disc diffusion method with E coli may be a little highly detected for the residual fluoroquinolones.
Abstract
This study was carried out to investigate the residue level of fluoroquinolones in hen's general eggs and specific eggs by microbiological assay method and high performance liquid chromatography (HPLC) method. HPLC separation was carried out by reversed phase chromatography on a Symmetry C18 (250 × 4.6 ㎜, 5 ㎛ particle size) with a phase composed of distilled water (containing 0.4% triethylamine and phosphoric acid) : Methanol (780 : 220, v/v), pumped isocratically at a flow rate of 1.0 ㎖/min. A fluorescence detector was utilized with an excitation wavelength of 278 ㎚ and an emission wavelength of 456 ㎚. The calibration curves were linear (γ2≥0.999) over a concentration range of 0.025～0.4 ㎍/㎖. Average recoveries of the five fluoroquinolones in whole eggs at fortified levels of 0.05～0.2 ㎍/g were ranged mean 78.1～91.7% and low coefficient of variation was less than 10% for all analysed samples. The limits of detection and limits of quantification for whole eggs were 1.2～6.0 ng/g and 2.3～9.1 ng/g, respectively. Only one hen's general eggfrom chicken farm in Incheon was detected with the residual fluoroquinolones (Microbiological assay method ; 1 of 47 general eggs) ; the range of residual concentration enrofloxacin was 0.12 ppm. Those in food stores were detected with the residual fluoroquinolones (Microbiological assay method ; 4 of 88 general eggs) ; the ranges of residual concentration enrofloxacin were 0.15～2.2 ppm, ciprofloxacin 0.01～0.06 ppm, and hen's specific eggs (40) in food stores were not detected. For the microbiological assay method of fluoroquinolones in hen's eggs, as the results of comparative analysis, the disc diffusion method with E coli may be a little highly detected for the residual fluoroquinolones.
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