두충과 느릅 혼합추출물에 의한 염증 억제 효과
In Vitro Anti-inflammatory Activities of Herbal Extracts with Eucommia ulimoides and Ulmus davidiana
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This study confirmed the cytotoxicity and anti-inflammatory activities of natural herbalextracts (HE) including Eucommia ulimoides and Ulmus davidiana against human mast cell(HMC-1). HE was extracted with distilled water (at 75C) and then freeze-dried for 5 days. Finally,the HE was sterilized by gamma radiation with 60Co γ source at room temperature. Cytotoxicityof the HE against HMC-1 cell was measured using cell counting kit-8 (CCK-8) assay. In addition,inflammatory cytokines such as TNF-α, IL-6 and IL-8 were evaluated by ELISA kit on the HMC-1 cells with calcimycin A23187 and phorbol 12-myristate 13-acetate (PMA). The results showedthat HE had no toxicity and reduced TNF-α, IL-6, IL-8 response on HMC-1 cells.
Abstract
This study confirmed the cytotoxicity and anti-inflammatory activities of natural herbalextracts (HE) including Eucommia ulimoides and Ulmus davidiana against human mast cell(HMC-1). HE was extracted with distilled water (at 75C) and then freeze-dried for 5 days. Finally,the HE was sterilized by gamma radiation with 60Co γ source at room temperature. Cytotoxicityof the HE against HMC-1 cell was measured using cell counting kit-8 (CCK-8) assay. In addition,inflammatory cytokines such as TNF-α, IL-6 and IL-8 were evaluated by ELISA kit on the HMC-1 cells with calcimycin A23187 and phorbol 12-myristate 13-acetate (PMA). The results showedthat HE had no toxicity and reduced TNF-α, IL-6, IL-8 response on HMC-1 cells.
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