TNF-α, IL-6, IL-8 저해를 통한 천연약용식물 추출물의항염증 특성
Anti-inflammatory Activity of Herbal Extracts through Inhibition of TNF-α, IL-6 and IL-8
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초록
This study evaluated the cytotoxicity and anti-inflammatory activities of natural herbalextracts including Houttuynia cordata and Eucommia ulmoides against human mast cell (HMC-1). Houttuynia cordata (HC) and Eucommia ulmoides (EU) were extracted with distilled water (at 75C)and then freeze-dried for 5 days. Finally, the mixture of HC and EU were sterilized by γ-rays irradiation. Cytotoxicity of the mixture against HMC-1 cell was measured using cell counting kit-8 (CCK-8) assay. In addition, inflammatory mediator cytokines such as TNF-α, IL-6 and IL-8 were evaluatedby ELISA kit on the HMC-1 cells with calcimycin A23187 and phorbol 12-myristate 13-acetate(PMA). The results showed that mixture of HC and EU had no cytoxicity and reduced TNF-α, IL-6,IL-8 response on HMC-1 cells.
Abstract
This study evaluated the cytotoxicity and anti-inflammatory activities of natural herbalextracts including Houttuynia cordata and Eucommia ulmoides against human mast cell (HMC-1). Houttuynia cordata (HC) and Eucommia ulmoides (EU) were extracted with distilled water (at 75C)and then freeze-dried for 5 days. Finally, the mixture of HC and EU were sterilized by γ-rays irradiation. Cytotoxicity of the mixture against HMC-1 cell was measured using cell counting kit-8 (CCK-8) assay. In addition, inflammatory mediator cytokines such as TNF-α, IL-6 and IL-8 were evaluatedby ELISA kit on the HMC-1 cells with calcimycin A23187 and phorbol 12-myristate 13-acetate(PMA). The results showed that mixture of HC and EU had no cytoxicity and reduced TNF-α, IL-6,IL-8 response on HMC-1 cells.
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