본문 바로가기
KERD
논문KCI 등재

蒼朮 에탄올 추출물이 비만세포 매개 염증반응에 미치는 영향

The Effects of Ethanol Extract from Atractylodes Chinensis Rhizome on the Mast Cell-Mediated Inflammatory Responses

김경준|김선민
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2011.04|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제24권 제1호|pp. 45-63

2

조회

0

다운로드

6

인용

0

참고문헌

키워드

MAPKsAtractylodes Chinensis Rhizome(ACR)The human mast cell line (HMC-1)NF-κB.
주제분류한의학

초록

Objective : Atractyloides Chinensis Rhizome (ACR) is widely used in oriental medicine as a remedy for an inflammation and an allergic disease. However, as yet there is no clear explanation of how ACR affects the production of inflammatory cytokine. This study was to determine the effects of ACR on the mast cell-mediated inflammatory responses. Method : The amount of inflammatory cytokine production induced by the phorbol myristate acetate (PMA) plus calcium ionophore(A23187) in the human mast cell line (HMC-1) incubated with various concentrations of ACR was measured. The TNF-α protein levels were analysised by Western blots. The TNF-α, IL-6 and IL-8 secreted protein levels were measured by the ELISA assay. The TNF-α, IL-6 and IL-8mRNA levels were measured by the RT-PCR analysis. NF-κB, phospho-IκB and MAPKs were examined by Western blot analysis. The NF-κB promoter activity was examined by a luciferase assay. Results : 1. The expressions of TNF-α and TNF-α mRNA were decreased dose-dependently at 0.05-0.2㎎/㎖ of ACR and significantly decreased at 0.2㎎/㎖. 2. The expressions of IL-6 and IL-6 mRNA were decreased dose-dependently at 0.05-0.2㎎/㎖ of ACR and significantly decreased at 0.2㎎/㎖. 3. The expressions of IL-8 and IL-8 mRNA were decreased dose-dependently at 0.05-0.2㎎/㎖ of ACR and significantly decreased at 0.2㎎/㎖ specially. 4. The expressions of Phosphorylated-JNK were decreased, not p38, ERK 5. The expressions of NF-κB were decreased dose-dependently at 0.1-0.2㎎/㎖ of ACR. The expressions of Phosphorylated IκB were significantly decreased at 0.2㎎/㎖. In addition, ACR suppressed PMA plus A23187-induced NF-κB promoting activity. Conclusion : It is suggested that ACR should suppress through inhibition of NF-kB activity and cytokine production.

Abstract

Objective : Atractyloides Chinensis Rhizome (ACR) is widely used in oriental medicine as a remedy for an inflammation and an allergic disease. However, as yet there is no clear explanation of how ACR affects the production of inflammatory cytokine. This study was to determine the effects of ACR on the mast cell-mediated inflammatory responses. Method : The amount of inflammatory cytokine production induced by the phorbol myristate acetate (PMA) plus calcium ionophore(A23187) in the human mast cell line (HMC-1) incubated with various concentrations of ACR was measured. The TNF-α protein levels were analysised by Western blots. The TNF-α, IL-6 and IL-8 secreted protein levels were measured by the ELISA assay. The TNF-α, IL-6 and IL-8mRNA levels were measured by the RT-PCR analysis. NF-κB, phospho-IκB and MAPKs were examined by Western blot analysis. The NF-κB promoter activity was examined by a luciferase assay. Results : 1. The expressions of TNF-α and TNF-α mRNA were decreased dose-dependently at 0.05-0.2㎎/㎖ of ACR and significantly decreased at 0.2㎎/㎖. 2. The expressions of IL-6 and IL-6 mRNA were decreased dose-dependently at 0.05-0.2㎎/㎖ of ACR and significantly decreased at 0.2㎎/㎖. 3. The expressions of IL-8 and IL-8 mRNA were decreased dose-dependently at 0.05-0.2㎎/㎖ of ACR and significantly decreased at 0.2㎎/㎖ specially. 4. The expressions of Phosphorylated-JNK were decreased, not p38, ERK 5. The expressions of NF-κB were decreased dose-dependently at 0.1-0.2㎎/㎖ of ACR. The expressions of Phosphorylated IκB were significantly decreased at 0.2㎎/㎖. In addition, ACR suppressed PMA plus A23187-induced NF-κB promoting activity. Conclusion : It is suggested that ACR should suppress through inhibition of NF-kB activity and cytokine production.

참고문헌 (46)

  1. Janeway C. A. Jr., "면역생물학(Immunobiology 6th edition)", pp.13~ (2005), 라이프사이언스
  2. Goldsby, R. A., "KUBY 면역학(Kuby immunology)", pp.263~ (2006), 월드사이언스
  3. Thomas PS, "Tumour necrosis factor-alpha: the role of this multifunctional cytokine in asthma", Immunol Cell Biol, 79, pp.132~140 (2001)
  4. Naka S, "Mast cells enhance T cell activation: importance of mast cell-derived TNF", Proc Natl Acad Sci USA, 102, pp.6467~6472 (2005)
  5. Bodreau RT, "Phosphatase inhibition potentiates IL-6 production by mast cells in response to Fcepsilon RI-mediated activation: involvement of p38 MAPK", J Leukoc Biol, 76, pp.1075~1081 (2004)
  6. Murayama T, "The immediate early gene 1 product of human cytomegalovirus is sufficient for upregulation of interleukin-8 gene expression", Biochem Biophys Res Commun, 279(1), pp.298~304 (2000)
  7. Arbabi S, "Mitogen-activated protein kinases", Crit Care Med, 30, pp.74~79 (2002)
  8. Beyaert R, "The p38", RK mitogen activat-ed protein kinase pathway regulates interleukin-6 synthesis response to tumor necrosis factor, 1996, pp.15, 1914~1923
  9. Kim SH, "Anti-allergic effects of Artemisia iwayomogi on mast cell-mediated allergy model", Exp Biol Med (Maywood), 230, pp.82~88 (2005)
  10. Kuprsh DV, "Convserved kappa B element located downstream of the tumor necrosis factor alpha gene: distinct NF-kappa B binding pattern and enhancer activity in LPS activated murine macrophages", Oncogene, 11, pp.97~106 (1995)
  11. Galien R, "Involvement of CCAAT", enhancer-binding protein and nuclear factor-kappa B binding site in interleukin-6 promoter inhibition by estrogens, 1996, pp.10, 713~722
  12. 박용기, "本草學", pp.334~335 (2004), 永林社
  13. 박종찬, "方藥合編중 蒼朮이 配伍된 方劑의 活用에 대한 考察", 대한한의학방제학회지, 11(2), pp.41~60 (2003)
  14. 임강민, "不換金正氣散이 마우스 Th1", Th2 分化 및 알레르기 염증 반응 조절에 미치는 효과, 2006, pp.20(6), 1467~1476[DOI]
  15. 한영규, "加味蒼栢散 Carrageenan유발 동물모델 관절염의 소염, 진통, 해열 및 면역에 미치는 영향", 한방재활의학과학회지, 15(1), pp.127~141 (2005)
  16. 이정우, "蒼朮이 천식 관련 Th1", Th2 세포 분비 cytokine에 미치는 영향, 2007, pp.28(4), 681~693[DOI]
  17. 허인희, "창출 추출물의 BV2 cell 소염작용에 관한 실험적 연구", 한방안이비인후피부과학회지, 20(2), pp.36~46 (2007), 대한한방안이비인후피부과학회
  18. Hachisuka H, "Purification of rat cutaneous mast cells with Percoll density centrifugation", Arch Dermatol Res, 280, pp.358~362 (1988)
  19. 이중달, "기본병리학", pp.79~130 (1996), 고려의학
  20. 강병수, "韓方臨床 알레르기", pp.57~58 (1988), 성보사
  21. Williams, C. M., "Mast cells can amplify airway reactivity and features of chronic inflammation in an asthma model in mice", J Exp Med, 192(3), pp.455~462 (2000)
  22. Ptak, W., "Initation of delayed-type hypersensitivity by low doses of monoclonal IgE antibody: Mediation by serotonin and inhibition by histamine", J Immunol, 170, pp.3929~3936 (1991)
  23. manna S.K., "Leflunomide suppresses TNF-induced cellular responses:effects on NF-kappaB, activator protein-1,c-jun N-terminal protein kinase, and apoptosis", J Immunol, 165(10), pp.5962~5969 (2000)
  24. Bokemeyer D, "Multiple intracellular MAP kinase signaling cascades", Kidney Int, 49, pp.1187~1198 (1996)
  25. Simon C., "The p38 SAPK pathway regulates the expression of themMP-9 collagenase via AP-1-dependent promotor activation", Exp Cell Res, 271(2), pp.344~355 (2001)
  26. Ip YT, "Signal transduction by the c-Jun N-terminal kinase(JNK)-from inflammation to development", Curr Opin Cell Biol, 10(2), pp.205~219 (1988)
  27. Waetzig V, "c-Jun N-terminal kinases (JNKs) mediate proinflammatory actions of microglia", Glia, 50(3), pp.235~246 (2005)
  28. Regnier, C. H., "Identification and characterization of an Ikappa kinase", Cell, 90, pp.373~383 (1997)
  29. Zandi, E., "The IkappaB kinase complex(IKK) cntains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation", Cell, 91, pp.243~252 (1997)
  30. Beg AA, "The I kappa B protein: multifunctional regulators of Rel", NF-kappa B transcription factor, 1993, pp.7(11), 2064~2070
  31. Palombella VJ, "The ubiquitinproteasome pathway is required for processing the NF- κB1 precursor protein and the activation of NF-κB", Cell, 78(5), pp.773~785 (1996)
  32. Baldwin, A. S. Jr, "The transcription factor NF-κB human disease", J Clin Ivest, 107, pp.3~6 (2000)
  33. Barnes, P. J., "Nuclear factor- κB: a pivotal transcription factor in chronic inflammatory disease", N Engl J Med, 336, pp.1066~1071 (1997)
  34. 김수영, "창출에 대한 문헌적 고찰", 한방재활의학회지, 7(1), pp.583~589 (1997)
  35. 한국생약학교수협의회, "한방약리학", pp.132~135 (1997), 정담
  36. 이용성, "經藥分類典", pp.18~ (2002), 정담출판사
  37. 이상인, "한약임상응용", pp.218~219 (1986), 전통의학연구소
  38. 강은미, "한국산 삽주의 기능성", 농산물저장유통학회지, 8(1), pp.86~91 (2001)
  39. Satoh K., "The effect of crude drugs using diuretic on horse kidney (Na+-K+)-adenosine triphosphatase", Yakugaku Zasshi, 111(2), pp.138~145 (1991)
  40. 김민자, "홍화 탄저병 방제 약제 선발", 한국자원식물학회지, 15(3), pp.36~40 (2002), 한국자원식물학회
  41. Hye-Young Sung, "Effects of Methanol Extracts of Rubus Coreanus Miquel and Atractylodes Japonica Koidzumi on Hepatic Toxicity and Immunomodulating Activity in Mice", Nutritional Sciences, 9(2), pp.68~73 (2006), 한국영양학회
  42. 유광원, "창출 성분의 장관 면역 자극을 통한 골수세포 증식활동", 한국식품과학회지, 115, pp.135~141 (2001)
  43. 김수용, "蒼朮 추출액이 肥滿白鼠와 3T3-Li의 지방세포분화에 미치는 영향", pp.1997
  44. 조항영, "蒼朮 알칼로이드의 진정작용에 관한 연구", 생약학회지, 5(3), pp.159~166 (1974)
  45. Butler DM, "Modulation ofproinflammatory cytokine release in rheumatoid synovial membrane cell cultures. Comparison of monoclonal anti TNF- alpha antibody with the interrukin-1 receptor antagonist", Eur Cytokine Netw, (4), pp.225~230 (1996)
  46. Erchler WB, "Age-associated increased interreukin-6 gene expression, late-life diseases, and frailty", Annu Rev Med, 51, pp.245~270 (2000)

관련 논문AI추천

魚腥草가 비만세포 매개 염증반응에 미치는 영향김경준, 이희조대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2009 · 제22권 제2호목단피의 NF-κB와 MAPKs 억제를 통한 Pro-inflammatory Cytokines 활성에 미치는 효과김경준, 박주호대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2009 · 제22권 제2호回生散 에탄올 추출물이 비만세포 매개성 염증반응에 미치는 영향권동렬, 박지혜, 이수경한의병리학회 · 동의생리병리학회지2014 · 제28권 제1호건칠이 비만세포 매개 염증반응에 미치는 영향김경준, 김석산대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2009 · 제22권 제1호Adenophorae Radix Attenuates Mast Cell-mediated Allergic Inflammation through Down-regulation of NF-κB/ Caspase-1 Activation명노일한국자원식물학회 · 한국자원식물학회지2020 · 제33권 제6호苦蔘 에탄올 추출물이 NFκB 및 JNK, p38 조절을 통한 알레르기성 염증에 미치는 영향박성식, 이지영사상체질면역의학회 · 사상체질면역의학회지2009 · 제21권 제1호

이 간행물의 다른 논문 (14건)

EBM 기반구축을 위한 방풍통성산 실험 연구 분석이준경, 김정훈, 신현규pp. 1-15知母에탄올추출물의 HT-29대장암세포 Apoptosis 유도효과박영규, 이부균, 이장천, 김태현, 김범호, 윤정록, 우원홍, 문연자, 임규상, 전병국pp. 16-24MSH에 의해 자극된 B16F10세포에서 사간(射干)의 멜라닌 합성 억제 효과이장천, 성병곤, 김대성, 우원홍, 임규상, 이부균pp. 25-35Propolis 투여가 Ovalbumin으로 유발된 마우스의 알러지성 천식에 미치는 영향맹드게렐, 박민철, 조은희, 변지환pp. 36-44Effects of Isatidis Radix and it's Active Component, Tryptanthrin on the Production of Inflammatory Mediators in Lipopolysaccharide-activated Raw264.7 Cells박숙자, 이종록, 조미정, 박상미, 변성희, 김상찬, 조일제pp. 64-77
원문 보기