Anti-inflammatory Effects of Water Extract of Eucommia ulmoides OLIVER on the LPS-induced RAW 264.7 Cells
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초록
Eucommia ulmoides OLIVER (EU) is a traditional Korean herbal used for the treatment of rheumatoid arthritis (RA). In the present study, the molecular pharmacology basis of its anti-inflammatory effect is revealed in this work, EU was studied in lipopolysaccharide (LPS)-activated macrophage cells (RAW 264.7) as an established inflammation model. After activation, nitric oxide (NO) production and iNOS mRNA were measured by using a colorimetric assay (Griess reagent), and reverse transcription polymerase chain reaction (RT-PCR), respectively. The change in the content of PGE2,TNFα, and IL-6 was concurrently monitored by ELISA. In results, we found that in the concentration range without showing cytotoxicity, EU produced a remarkable anti-inflammatory effect and showed a dose-dependent inhibition of LPSinduced NO production. Compared with indomethacin, EU has more potency and a specific action of NO inhibition, PGE2,IL-6, and TNF-α inhibition. These results suggest that EU may be a suitable herbal medicine to yield the greatest antiinflammatory activity for food additives and medicine.
Abstract
Eucommia ulmoides OLIVER (EU) is a traditional Korean herbal used for the treatment of rheumatoid arthritis (RA). In the present study, the molecular pharmacology basis of its anti-inflammatory effect is revealed in this work, EU was studied in lipopolysaccharide (LPS)-activated macrophage cells (RAW 264.7) as an established inflammation model. After activation, nitric oxide (NO) production and iNOS mRNA were measured by using a colorimetric assay (Griess reagent), and reverse transcription polymerase chain reaction (RT-PCR), respectively. The change in the content of PGE2,TNFα, and IL-6 was concurrently monitored by ELISA. In results, we found that in the concentration range without showing cytotoxicity, EU produced a remarkable anti-inflammatory effect and showed a dose-dependent inhibition of LPSinduced NO production. Compared with indomethacin, EU has more potency and a specific action of NO inhibition, PGE2,IL-6, and TNF-α inhibition. These results suggest that EU may be a suitable herbal medicine to yield the greatest antiinflammatory activity for food additives and medicine.
참고문헌 (26)
- 박종필, "Suppression Effect of the Inflammatory Response in Macrophages by Paeoniae Radix Rubra Extracts", 한국약용작물학회지, 19(5), pp.373~379 (2011), 한국약용작물학회
- Bowen WP, "Nonlinear-regression using spreadsheets", Trends in Pharmacological Sciences, 16, pp.413~417 (1995)
- Diehl S, "The two faces of IL-6 on Th1", Th2 differentiation, 2002, pp.39, 531~536
- Deyama T, "Constituents and pharmacological effects of Eucommia and Siberian ginseng", Acta pharmacologica Sinica, 22, pp.1057~1070 (2001)
- Greenway F, "A clinical trial testing the safety and efficacy of a standardized Eucommia ulmoides Oliver bark extract to treat hypertension", Alternative Medicine Review, 16, pp.336~347 (2011)
- nitrate in biological fluids, "Analytical Biochemistry", 1982, 131~138
- Gu J, "Effects of lignans extracted from Eucommia ulmoides and aldose reductase inhibitor epalrestat on hypertensive vascular remodeling", Journal of Ethnopharmacology, 133, pp.6~13 (2011)
- Hesslinger C, "Inhibition of inducible nitric oxide synthase in respiratory diseases", Biochemical Society Transactions, 37, pp.886~891 (2009)
- Hsieh CL, "Antioxidant actions of Du-zhong (Eucommia ulmoides OLIV.) toward oxidative damage in biomolecules", Life Sciences, 66, pp.1387~1400 (2000)
- 정용준, "상황버섯과 발아현미상황버섯 열수추출물의 Murin Macrophage RAW 264.7 세포에서 항염증 반응 비교", 한국약용작물학회지, 17(2), pp.97~101 (2009), 한국약용작물학회
- Bong Hyun Kim, "Elucidation of Anti-inflammatory Potencies of Eucommia ulmoides Bark and Plantago asiatica Seeds", Journal of Medicinal Food, 12(4), pp.764~769 (2009), 한국식품영양과학회
- Kim HM, "The nitric oxide-producing activities of nitric oxide in mouse peritoneal macrophages after priming with interferongamma by the stem of Sinomenium acutum", Journal of Ethnopharmacology, 66, pp.311~317 (1999)
- Kim SJ, "Antiinflammatory activity of gumiganghwaltang through the inhibition of nuclear factor-kappa B activation in peritoneal macrophages", Biological & Pharmaceutical Bulletin, 28, pp.233~237 (2005)
- Kumar P, "Correlation of cytokine and mobility in mice with arthritis and during therpy with Swertia chirayita", Journal of Herbal Pharmacotherapy, 4, pp.33~45 (2004)
- 권승환, "Neuroprotective effects of Eucommia ulmoides Oliv. Bark on amyloid beta(25-35)-induced learning and memory impairments in mice", NEUROSCIENCE LETTERS, 487(1), pp.123~127, ELSEVIER IRELAND LTD
- 이용규, "대식세포주 RAW264.7 세포에서 Curcumin의 Lipopolysaccharide 에 의한 Nitric Oxide 생성 억제 효과", 한국약용작물학회지, 15(6), pp.451~456 (2007), 한국약용작물학회
- Lie H, "Application of ultrasonic technique for extracting chlorogenic acid from Eucommia ulmodies Oliv", Ultrasonics Sonochemistry, 12, pp.295~300 (2005)
- Lin J, "Eucommia ulmoides Oliv. antagonizes H2O2-induced rat osteoblastic MC3T3-E1 apoptosis by inhibiting expressions of caspases 3, 6, 7, and 9", Journal of Zhejiang University Science B, 12, pp.47~54 (2011)
- 이승은, "약용식물자원 추출물의 항염증활성", 한국약용작물학회지, 19(4), pp.217~226 (2011), 한국약용작물학회
- Medzhitov R, "Innate immunity: the virtues of a nonclonal system of recognition", Cell, 91, pp.295~298 (1997)
- Morrison DC, "Endotoxins and disease mechanisms", Annual Review of Medicine, 38, pp.417~432 (1987)
- Nishimoto N, "Interleukin 6: from bench to bedside. Nature clinical practice", Rheumatology, 2, pp.619~626 (2006)
- Park HJ, "Syringin 4-O-b-glucoside, a new phenyprosynthase inhibitor, from the stem bark of Magnolia sieboldii", Journal of Natural Products, 59, pp.1128~1130 (2004)
- Sacco RE, "Comparative nitric oxide production by LPS-stimulated monocyte-derived macrophages from Ovis canadensis and Ovis aries", Comparative Immunology, Microbiology and Infectious Diseases, 29, pp.1~11 (2006)
- Shin M, "A molecular pharmacology study into the anti-inflammatory actions of Eluphorbia hirta L. on the LPS-induced RAW 264.7 cells through selective iNOS protein inhibition", Journal of Natural Medicine, 64, pp.330~335 (2010)
- Zhu HW, "A protein-bound polysaccharide from the stem bark of Eucommia ulmoides and its anti-complementary effect", Carbohydrate Research, 344, pp.1319~1324 (2009)