RAPD 방법을 이용한 국내 수입 인삼(Panax ginseng C. A. meyer)의 다양성 분석
Analysis of Diversity of panax ginseng collected in korea by RAPD technique
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Abstract
Genetic differences among nine land races of Korean ginseng (Panax ginseng C. A. Meyer) were examined using RAPD markers. Land races of Korean ginseng were collected from nine regions in Korea: Cheongwon, Guesan, Geumsan, Namwon, Pochun, Yangju, Yeoncheon, Yeogju. Out of 48 RAPD primers tested, 5 primers (OPA 7, OPA 13, URP 2, URP 3 AND UBC 3) produced remarkable bands which showing polymrophisms among evaluated collections. Lower levels of genetic diversity were in detected same land races than among other land races. Genetic differences within and among land races indicate heterogeneity. These results indicate that cultivated ginseng in Korea is heterogeneous. Genetic similarity matrices of RAPD profiles were generated via coefficients of variation and the data were processed by the cluster analysis(UPGMA). When 90 collections were evaluated using selected 5 primers, those were clustered to 5 and 3 subgroups. These differences in geenetic variation between land races of Korean ginseng implied the potential source for further breeding of Korean ginseng.