백두산지역과 국내 더덕 수입종의 RAPD에 의한 유연관계 분석

Abstract

Extracted genomic DNA from 16 accessions of Condonopsis lanceolata collected from south Korea and the Baekdoo Mt. areas of China were analyzed for their genetic relationships by RAPD. Twenty 10-mer-oligonucleotide primers having reproductive polymorphism were selected for the RAPD analysis. The size of amplified DNA was almost between 125 bp and 2.0 kbp. Sixteen collected Codonopsis lanceloata were analyzed with 20 primers which generated 73(49.3%) polymorphic bands among 148 PCR products. The mean number of polymorphic bands were 7.4 and vaired 1~9 per primer. It was, thus, demonstrated that RAPD was useful for detecting polymorphism in Condonopsis lanceloata. The range of 1-F valus(genetic similarity) was from 0.682 to 0.959. These results indicate variable genetic similarities> By UPGMA (Unweighted Pair Group Method using an Arithmetic average) cluster analysis based on 1-F value, genetic distance among the 6 collected Codonopsis lanceolata was 0.133~0.400. It was certainly classified into two groups between collected accessions from Korea and China, and the genetic distance was about 0.281. Both accessions collected from Korea and China showed miner differences, while the genetic relationships of Tonghua Xian and Liuhe Xian from China was farthest with other accessions collected.