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RAPD를 이용한 고려인삼 육성계통의 유전적 다양성 분석

Genetic variation in pure lines of panax ginseng based on by RAPD analysis

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Abstract

This experiment was conducted to evaluate the diversity and purity of the Korean ginseng (Panax ginseng) lines developed by the pure line selection using RAPD markers. Four primer (OPA 19, OPM11, URP 3 and UBC 98) out of the 48 primer tested produced band which showed within-line polymorphisms at least in one line. Within-line polymorphisms were detected in six lines by OPA 19, in four lines by URP 03,in five lines by OPM 11, and in one line by UBC 98 respectively. Five plants obtained from the commercial cultivar "Chenpung" were differentiated using the primer OPA 19 and OPM 11. Five plants obtained from the "Yeonpung" were differentiated using the primer OPM 11. Detection of within-line RAPD polymorphi는 might be attributed to the fact that cross pollination appear in P. ginseng and a long period of three to four years required to reach the reproductive stage thereby delay the process to homozygosity.

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