액아배양을 통한 고추나물의 대량증식
Micropropagation of Hypericum erectum by Axillary Bud Culture
2
조회
0
다운로드
6
인용
0
참고문헌
초록
Abstract
One of micropropagation methods was investigated by using a multiple-shoots protocol. Multiple shoot formation was obtained from excised axillary buds of Hypericum erectum on half-strength or basal MS medium supplemented with TDZ or BA. The optimal combination of shoot multiplication for the production of more shoots with a suitable size was MS medium supplemented with 0.005 ㎎ · L.1 TDZ (6.5 adventitious shoots per node). In vitro rooting was carried on halfstrength MS medium with 1 ㎎·L.1 GA3 and 0.5 ㎎·L.1 IBA treatment. In addition, the rooted cuttings were showed a better root growth in the greenhouse and survived in more than 90%. The results show that the species can be micropropagated effectively by the application of axillary bud culture systems.
참고문헌 (14)
- Daykin A, "Effect of gibberellin on the cellular dynamics of dwarf pea internode development", Planta, 203, pp.526~535 (1997)
- Gibson DM, "Boosting the quality and potency of St. John's wort, in: Agricultural Research", pp.12~13 (2000), Ithaca, NY, Camilo Cannel. Oxford University Press
- Kang HJ, "Micropropagation of Corylopsis coreana by thidiazuron treatment", Korean J. Plant Biotechnology, 30(3), pp.263~267 (2003)
- Kim KS, "Micropropagation of Achyranthes Japonica through axillary bud culture", Korean. J. Plant Tissue Culture, 24(6), pp.357~360 (1997)
- 황백, "고추나물의 Hypericin 함량", 한국약용작물학회지, 13(3), pp.101~104 (2005), 한국약용작물학회
- Kim SH, "Analysis of genetic relationships in Hypericum erectum Thunb", RAPD. Korean J. Medicinal Crop Sci., 13(4), pp.141~145 (2005)
- Kirdmanee C, "Effects of CO2 enrichment and supporting material in vitro on photoautotrophic growth of Eucalyptus plantlets in vitro and ex vitro", Vitro Cell Dev Biol-plant, 31, pp.144~149 (1995)
- Moon HK, "Micropropagation via axillary bud induction of Eucalyptus pellita", Korean J. Plant Biotechnology, 30(3), pp.269~273 (2003)
- 문흥규, "음나무의 액아배양에 의한 기내증식", 한국임학회지, 91(6), pp.775~780 (2002), 한국임학회
- Murthy BNS, "Review-thidiazuron: a potent regulator of in vitro plant morphogenesis", Vitro Cell Dev Biol-Plant, 34, pp.267~275 (1998)
- Schempp CM, "Inhibition of tumour cell growth by hyperforin, a novel anticancer drug from St. John's wort that acts by induction of apoptosis", Oncogene, 21, pp.1242~1250 (2002)
- Shim KK, "Mass propagation of Korean navive Styrax japoni through axillary bud culture", J. Korean. Soc. Hort. Sci, 38(5), pp.575~580 (1997)
- Shim KK, "Mass propagation of Betula pendula ‘Purpurea’ through axilary bud culture", J. Korean Soc. Hort. Sci, 38(6), pp.776~782 (1997)
- 김태정, "한국의 자원식물", pp.116~118 (1996), 서울대학교 출판부