고추냉이 정단배양에 있어서 BA 농도 및 배양방법에 따른 기내증식 효과
Effect of BA Concentrations and Culture Methods on in Vitro Plant Multiplication from Shoot-Tip Culture of Wasabia japonica
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Abstract
Effect of BA concentrations and culture methods on in vitro plant multiplication from shoot-tip cultures of Wasabia japonica was studied. Shoot-tips with leaf primordia and apical meristem were cultured on MS basal medium for all the experiments. Liquid medium for 2 weeks followed by semi-solid medium for 4 weeks containing 1.0 mg/L BA was the best to number of shoots (22.8) and shoot length (3.5 cm). Shoots proliferated could be divided into ca. 5 to 11 of cultures for the multiplication of plantlets. Divided plantlets showed root formation (90%) well onto MS basal medium without growth regulators like IBA and NAA. After rooting, all the plantlets transferred into the pots containing composed soil (bio-media Co., peatmoss 8～10%, coir dust 66～70%, zeolite 13～17%, vermiculite 3～7%, perlite 2～4%) and grown well into whole plants with multiple shoots.