섬시호(Bupleurum latissimum Nakai)의 조직배양을 통한 대량생산

Abstract

- This study was carried out to establish the micropropagation system of Bupleurum latissimumNakai that is aKorean native endangered species. Callus were induced from the leaf, petiole and floral bud and the percentage of callusformation was highest in the floral bud on the MS medium containing 2.0 mg· L-1 2,4-D. Especially, callus inducedfrom floral bud was formed 77.8% and the percentage of shoot formation was 42.6% on the MS medium containing 2.0mg· L-12,4-D plus 1.0 mg· L-1TDZ. For simultaneously callus formation and shoot regeneration, 1/2 MS medium wasmore effective than MS medium. The percentage callus formation, shoot regeneration and rooting were 46.3%, 13.0%,13.0% in 1/2 MS medium, respectively. Soot regeneration from callus was good in 1/2 MS medium supplemented with2.0 mg· L-12,4-D plus 1.0 mg· L-1BA where percentage of shoot regeneration was 74.1%, and the number of shoot perexplant was 2.4. The percentage of rooting was lowest (57.8%) in control while it was highest (97.8%) in 1.5 mg· L-1NAA. In acclimatization of regenerated plantlets, the percentage of survived plantlets was highest (86.1%), and plantheight, root length and fresh weight were good in the soil for horticulture.Key words - Bupleurum latissimum, Callus, Shoot, Regeneration, Propagation韓資植誌 Korean J. Plant Res. 20(4)：367∼374(2007)한 거의 이루어진 적이 없다.따라서 본 연구는 멸종 위기에 처한 섬시호의 대량 증식을 위해 조직배양을 통한 번식체계를 확립하고자 적합한 배양 절편체를 선정하고 선정된 절편체로부터 캘러스 유도, 식물체 재분화,발근에 효과적인 식물생장조절물질의 종류와 농도를 구명하며재분화 식물체의 생존율을 향상시킬 수 있는 순화용토를 선발하고자 하였다.재료 및 방법식물재료본 실험에서 사용한 섬시호는 종자를 2005년 8월 환경부 서식지외 보전기관인 기청산식물원에서 채취하여 전북대학교 온실에서 발아시킨 후 실험재료로 사용하였다.치상 절편체 부위 및 생장조절물질에 따른 캘러스 유도캘러스 형성률이 높은 치상 절편체를 찾기 위하여 식물체가약 30cm로 자랐을 때 5∼7번째의 잎과 엽병을 채취하여 잎은 5