인플루엔자 바이러스에 대한 Zanthoxylum속의 항바이러스 효과 검정
Antiviral Activity of Zanthoxylum Species against Influenza Virus
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초록
We aimed to investigate the antiviral activity of Zanthoxylum species against influenza virus A/WS/33, A/PR/ 8 and B/Lee/40 used by sulforhodamine B (SRB) assay and the action of leaves extracts of Zanthoxylum piperitum on life cycle of influenza virus A/WS/33. Among the twelve extracts, only the leaf extract of Z. piperitum exhibited strong antiviral activity at low concentration of less than 10㎍/㎖ with no citotoxicity (50㎍/㎖) against all of three viruses. In addition, only oseltamivir showed antiviral activity with IC50 of 65.3㎍/㎖ against influenza A/WS/33 among the viruses. Furthermore, the leaf extract of Z. piperitum suppressed infection of influenza virus A/WS/33, when added just prior (−1 hr) or after virus inoculation (0 hr). Leaf extract of Z. piperitum directly affect the infectivity of influenza virus A/WS/33 particles. Therefore, Leaf extract of Z. piperitum exhibited higher antiviral activity against three influenza viruses than that of the oseltamivir, which directly interacts with influenza A/WS/33 particles, affecting the initial stages of infection such as receptor binding and virus entry.
Abstract
We aimed to investigate the antiviral activity of Zanthoxylum species against influenza virus A/WS/33, A/PR/ 8 and B/Lee/40 used by sulforhodamine B (SRB) assay and the action of leaves extracts of Zanthoxylum piperitum on life cycle of influenza virus A/WS/33. Among the twelve extracts, only the leaf extract of Z. piperitum exhibited strong antiviral activity at low concentration of less than 10㎍/㎖ with no citotoxicity (50㎍/㎖) against all of three viruses. In addition, only oseltamivir showed antiviral activity with IC50 of 65.3㎍/㎖ against influenza A/WS/33 among the viruses. Furthermore, the leaf extract of Z. piperitum suppressed infection of influenza virus A/WS/33, when added just prior (−1 hr) or after virus inoculation (0 hr). Leaf extract of Z. piperitum directly affect the infectivity of influenza virus A/WS/33 particles. Therefore, Leaf extract of Z. piperitum exhibited higher antiviral activity against three influenza viruses than that of the oseltamivir, which directly interacts with influenza A/WS/33 particles, affecting the initial stages of infection such as receptor binding and virus entry.
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