In Vitro Antiviral Activity of Cinnamomum cassia and Its Nanoparticles Against H7N3 Influenza A Virus
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초록
Nanoparticles have wide-scale applications in various areas, including medicine, chemistry, electronics, and energy generation. Several physical, biological, and chemical methods have been used for synthesis of silver nanoparticles. Green synthesis of silver nanoparticles using plants provide advantages over other methods as it is easy, efficient, and eco-friendly. Nanoparticles have been extensively studied as potential antimicrobials to target pathogenic and multidrug-resistant microorganisms. Their applications recently extended to development of antivirals to inhibit viral infections. In this study, we synthesized silver nanoparticles using Cinnamomum cassia (Cinnamon) and evaluated their activity against highly pathogenic avian influenza virus subtype H7N3. The synthesized nanoparticles were characterized using UVVis absorption spectroscopy, scanning electron microscopy, and Fourier transform infrared spectroscopy. Cinnamon bark extract and its nanoparticles were tested against H7N3 influenza A virus in Vero cells and the viability of cells was determined by tetrazolium dye (MTT) assay. The silver nanoparticles derived from Cinnamon extract enhanced the antiviral activity and were found to be effective in both treatments, when incubated with the virus prior to infection and introduced to cells after infection. In order to establish the safety profile, Cinnamon and its corresponding nanoparticles were tested for their cytotoxic effects in Vero cells. The tested concentrations of extract and nanoparticles (up to 500 μg/ml) were found non-toxic to Vero cells. The biosynthesized nanoparticles may, hence, be a promising approach to provide treatment against influenza virus infections.
Abstract
Nanoparticles have wide-scale applications in various areas, including medicine, chemistry, electronics, and energy generation. Several physical, biological, and chemical methods have been used for synthesis of silver nanoparticles. Green synthesis of silver nanoparticles using plants provide advantages over other methods as it is easy, efficient, and eco-friendly. Nanoparticles have been extensively studied as potential antimicrobials to target pathogenic and multidrug-resistant microorganisms. Their applications recently extended to development of antivirals to inhibit viral infections. In this study, we synthesized silver nanoparticles using Cinnamomum cassia (Cinnamon) and evaluated their activity against highly pathogenic avian influenza virus subtype H7N3. The synthesized nanoparticles were characterized using UVVis absorption spectroscopy, scanning electron microscopy, and Fourier transform infrared spectroscopy. Cinnamon bark extract and its nanoparticles were tested against H7N3 influenza A virus in Vero cells and the viability of cells was determined by tetrazolium dye (MTT) assay. The silver nanoparticles derived from Cinnamon extract enhanced the antiviral activity and were found to be effective in both treatments, when incubated with the virus prior to infection and introduced to cells after infection. In order to establish the safety profile, Cinnamon and its corresponding nanoparticles were tested for their cytotoxic effects in Vero cells. The tested concentrations of extract and nanoparticles (up to 500 μg/ml) were found non-toxic to Vero cells. The biosynthesized nanoparticles may, hence, be a promising approach to provide treatment against influenza virus infections.
참고문헌 (65)
- Ankamwar B, "Gold nanotriangles biologically synthesized using tamarind leaf extract and potential application in vapor sensing", Syn. React. Inorg. Met, 35, pp.19~26 (2005)
- Ankanna S, "Production of biogenic silver nanoparticles using Boswellia ovalifoliolata stem bark", Dig. J. Nanomater. Biostruct, 5, pp.369~372 (2010)
- Bae CH, "Formation of silver nanoparticles by laser ablation of a silver target in NaCl solution", Appl. Surf. Sci, 197, pp.628~634 (2002)
- Baram-Pinto D, "Inhibition of HSV-1 attachment, entry, and cell-to-cell Spread by functionalized multivalent gold nanoparticles", Small, 6, pp.1044~1050 (2010)
- Baram-Pinto D, "Inhibition of herpes simplex virus type 1 infection by silver nanoparticles capped with mercaptoethane sulfonate", Bioconjug. Chem, 20, pp.1497~1502 (2009)
- Chandran SP, "Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract", Biotechnol. Prog, 22, pp.577~583 (2006)
- Choi YK, "Continuing evolution of H9N2 influenza viruses in Southeastern China", J. Virol, 78, pp.8609~8614 (2004)
- Cralg, WJ, "Health-promoting properties of common herbs", Amer. J. Clin. Nutr, 70, pp.4915~4995 (1999)
- Da Costa AO, "Comparative analysis of three methods to assess viability of mammalian cells in culture", Biocell, 23, pp.65~72 (1999)
- De Clercq E, "Antiviral drugs in current clinical use", J. Clin. Virol, 30, pp.115~133 (2004)
- Elechiguerra JL, "Interaction of silver nanoparticles with HIV-1", J. Nanobiotechnology, 3, pp.1~10 (2005)
- Esteban D, "Mechanisms of viral emergence", Vet. Res, 41, pp.38~ (2010)
- Fayaz AM, "Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection", Int. J. Nanomedicine, 7, pp.5007~5018 (2012)
- Ferguson NM, "Strategies for containing an emerging influenza pandemic in Southeast Asia", Nature, 437, pp.209~214 (2005)
- Fouchier RA, "Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome", Proc. Natl. Acad. Sci. USA, 101, pp.1356~1361 (2004)
- Galdiero S, "Frontiers of Nanomedical Research", pp.565~594 (2014), Worlds Scientific Publishing
- Gan PP, "Potential of plant as a biological factory to synthesize gold and silver nanoparticles and their applications", Rev. Environ. Sci. Biotechnol, 11, pp.169~206 (2012)
- Geethalakshmi R, "Synthesis of plantmediated silver nanoparticles using Trianthema decandra extract and evaluation of their anti-microbial activities", Int. J. Eng. Sci. Technol, 2, pp.970~975 (2010)
- Ha Y, "X-ray structures of H5 avian and H9 swine influenza virus hemagglutinins bound to avian and human receptor analogs", Proc. Natl. Acad. Sci. USA, 98, pp.11181~11186 (2001)
- Hayashi K, "Inhibitory effect of cinnamaldehyde, derived from Cinnamomi cortex, on the growth of influenza A", PR, Antiviral Res, pp.2007, 74[DOI]
- Huang J, "Biosynthesis of silver and gold nanoparticles by novel sun dried Cinnamomum camphora leaf", Nanotechnology, 18, pp.105~106 (2007)
- Kinoshita E, "Anti-influenza virus effects of elderberry juice and its fractions", Biosci. Biotechnol. Biochem, 76, pp.1633~1638 (2012)
- Klaus T, "Silverbased crystalline nanoparticles, microbially fabricated", Proc. Natl. Acad. Sci. USA, 96, pp.13611~13614 (1999)
- Ko HC, "The effect of medicinal plants u sed in Chinese folk medicine on RANTES secretion by virus-infected human epithelial cells", J. Ethnopharmacol, 107, pp.205~210 (2006)
- Konishi Y, "Bioreductive deposition of platinum nanoparticles on the bacterium Shewanella algae", J. Biotechnol, 128, pp.648~653 (2007)
- Kubo T, "Antipyretic effect of Mao-to, a Japanese herbal medicine, for treatment of type A influenza infection in children", Phytomedicine, 14, pp.96~101 (2007)
- Kwon HJ, "In Vitro inhibitory activity of Alpinia katsumadai extracts against influenza virus infection and hemagglutination", Virol. J, 7, pp.307~ (2010)
- Lara HH, "Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds", J. Nanobiotechnology, 9, pp.30~ (2011)
- Lee JB, "Anti-influenza A virus effects of fructan from Welsh onion (Allium fistulosum L.)", Food Chem, 134, pp.2164~2168 (2012)
- Liu YC, "New pathway for the synthesis of ultrafine silver nanoparticles from bulk silver substrates in aqueous solutions by sonoelectrochemical methods", Electrochem. Commun, 6, pp.1163~1168 (2004)
- Lu L, "Silver nanoparticles inhibit hepatitis B virus replication", Antivir. Ther, 13, pp.253~262 (2008)
- Mallick K, "Self-assembly of silver nanoparticles in a polymer solvent: formation of a nanochain through nanoscale soldering", Mater. Chem. Phys, 90, pp.221~224 (2005)
- Mallikarjun K, "Green synthesis of silver nanoparticles using Ocimum leaf extract and their characterization", Dig. J. Nanomater. Biostruct, 6, pp.181~186 (2011)
- Mantani N, "Inhibitory effect of Ephedrae herba, an oriental traditional medicine, on the growth of influenza A", PR, Antiviral Res, pp.1999, 44[DOI]
- Jancy ME, "Green synthesis and characterization of nano silver using leaf extract of Morinda pubescens", Asian J. Pharm. Clinical Res, 5, pp.159~162 (2012)
- Mori Y, "Antiviral activity of silver nanoparticles", chitosan composites against H1N1 influenza A virus, 2013, pp.8, 93~
- Nair B, "Coalescence of nanoclusters and formation of submicron crystallites assisted by Lactobacillus strains", Cryst. Growth Des, 2, pp.293~298 (2002)
- Noh HJ, "Antimicrobial activity and increased freeze-drying stability of sialyllactose-reduced silver nanoparticles using sucrose and trehalose", J. Nanosci. Nanotechnol, 12, pp.3884~3895 (2012)
- Papp I, "Inhibition of Influenza virus infection by multivalent Sialic-acid-functionalized Gold nanoparticles", Small, 6, pp.2900~2906 (2010)
- Parak WJ, "Biological applications of colloidal nanocrystals", Nanotechnology, 14, pp.15~27 (2003)
- Peyre M, "Avian influenza vaccines: a practical review in relation to their application in the field with a focus on the Asian experience", Epidemiol. Infect, 137, pp.1~21 (2009)
- Poland GA, "Influenza virus resistance to antiviral agents: a plea for rational use", Clin. Infect. Dis, 48, pp.1254~1256 (2009)
- Prathap SC, "Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract", Biotechnol. Prog, 22, pp.577~583 (2006)
- Reed LJ, "A simple method of estimating fifty percent endpoints", Am. J. Hyg, 27, pp.493~497 (1938)
- Rogers JV, "A preliminary assessment of silver nanoparticle inhibition of monkeypox virus plaque formation", Nanoscale Res. Lett, 3, pp.129~133 (2008)
- Sandmann G, "Preparation of silver nanoparticles on ITO surfaces by a double-pulse method", J. Electroanal. Chem, 491, pp.78~86 (2000)
- Shankar SS, "Rapid synthesis of Au, Ag and bimetallic Au core-Ag shell nanoparticles by using neem (Azadirachta indica) leaf broth", J. Colloid. Interface Sci, 275, pp.496~502 (2004)
- Shankar SS, "Controlling the optical properties of lemongrass extract synthesized gold nanotriangles and potential application in infraredabsorbing optical coatings", Chem. Mater, 17, pp.566~572 (2005)
- Shivshankar S, "Geranium leaf assisted biosynthesis of silver nanoparticles", Biotechnol. Prog, 19, pp.1627~1631 (2003)
- Sivaraman SK, "A green protocol for room temperature synthesis of silver nanoparticles in seconds", Curr. Sci, 97, pp.1055~1059 (2009)
- Skehel JJ, "Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin", Ann. Rev. Biochem, 69, pp.531~569 (2000)
- Smetana AB, "Synthesis of spherical silver nanoparticles by digestive ripening, stabilization with various agents, and their 3-D and 2-D superlattice formation", J. Colloid. Interface Sci, 284, pp.521~526 (2005)
- Sondi I, "Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for gram-negative bacteria", J. Colloid. Interface Sci, 275, pp.177~182 (2004)
- Speshock JL, "Interaction of silver nanoparticles with Tacaribe virus", J. Nanobiotechnology, 8, pp.19~ (2010)
- Sriwilaijaroen N, "Antiviral effects of Psidium guajava Linn (guava) tea on the growth of clinical isolated H1N1 viruses: its role in viral hemagglutination and neuraminidase inhibition", Antivir. Res, 94, pp.139~146 (2012)
- Sun L, "Silver nanoparticles inhibit replication of respiratory syncytial virus", J. Biomed. Nanotechnol, 4, pp.149~158 (2008)
- Swamy MK, "The green synthesis, characterization, and evaluation of the biological activities of silver nanoparticles synthesized from Leptadenia reticulate leaf extract", Appl. Nanosci, 5, pp.73~81 (2015)
- Vorobyova SA, "Preparation of silver nanoparticles by interphase reduction. Colloids Surf. A: Physicochem", Eng. Aspects, 152, pp.375~379 (1999)
- Webster RG, "Evolution and ecology of influenza A viruses", Microbiol. Rev, 56, pp.152~179 (1992)
- Wei D, "Facile synthesis of Ag and Au nanoparticles utilizing chitosan as a mediator agent", Colloids Surf. B Biointerface, 62, pp.136~142 (2008)
- Willner I, "Growing metal nanoparticles by enzymes", Adv. Mater, 18, pp.1109~1120 (2006)
- 원지나, "Antiviral Activity of the Plant Extracts from Thuja orientalis, Aster spathulifolius, and Pinus thunbergii Against Influenza Virus A", PR, 34, pp.Journal of Microbiology and Biotechnology, 2013[DOI]
- Xia Y, "Shape-controlled synthesis and surface plasmonic properties of metallic nanostructures", MRS Bull, 30, pp.338~348 (2005)
- Xiang DX, "Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro", J. Virol. Methods, 178, pp.137~142 (2011)
- Yu DG, "Formation of colloidal silver nanoparticles stabilized by Na+-poly (-glutamic acid) silver nitrate complex via chemical reduction process", Colloids Surf. B Biointerfaces, 59, pp.171~178 (2007)