자외선이 조사된 인간피부섬유아세포에 복분자 추출물이 미치는 영향
Effects of Rubus coreanus Extracts on Ultraviolet-A Irradiated Cultured Human Skin Fibroblasts
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Abstract
Sunlight, and in particular its UV component, is the major environmental trigger that underlies the major signs of human skin and skin cancer in general. Therefore, this study was carried out to investigate the UV protection effects of R. coreanus. R. coreanus was extracted by ultra high pressure extraction process at 500 ㎫ and 30℃ for 5 and 15 minutes. The cytotoxicity of the extracts extracted by ultra high pressure process on human dermal fibroblast cell CCD-986sk, human kidney normal cell HEK293, and human lung normal cell HEL299 was measured as 17.5%, 16.5% and 14.0%, respectively in adding 1.0 ㎎/㎖ of the samples, which was much lower than that from conventional water extraction method at 100℃ as 23.2%, 22.5%, 21.2%. The secretion of NO− from macrophage showed 15.9 μM on the R. coreanus extract from this process, which was higher than others. Prostaglandin E2 (PGE2) production from UV-induced human skin cells was also greatly decreased down to 510 pg/㎖, compared to the control. From the results, we considered that the extracts from R. coreanus could be potent natural materials for skin anti-inflammation agent, and could be used as a potential anti-aging for the photo-damaged skin.
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