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Protective Effect of an Ethanol Extract Mixture of Curcuma longae Radix, Phellinus linteus, and Scutellariae Radix on Oxidative Neuronal Damage

김승환|김주연|이홍규|성연희|배기환|권기연|유재국
한국약용작물학회|2011.02|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제19권 제1호|pp. 31-37

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참고문헌

키워드

Scutellariae RadixNeuroprotectionPhellinus linteusCurcuma longae RadixH_2O_2Cultured Cortical NeuronsCerebral Ischemic Injury

초록

Previous work demonstrated that an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix markedly inhibits Aβ (25-35)-induced neurotoxicity. The present study was performed to further verify the neuroprotective effect of HS0608 on oxidative and ischemic cerebral injury using cultured rat cortical neurons and rats. Exposure of cultured cortical neurons to 100 μM hydrogen peroxide (H_2O_2) induced neuronal apoptotic death. At 10-100㎍/㎖, HS0608 inhibited neuronal death, elevation of intracellular calcium concentration ([Ca^(2+)]i), and generation of reactive oxygen species (ROS) induced by H_2O_2 in primary cultures of rat cortical neurons. In vivo, HS0608 prevented cerebral ischemic injury induced by 2-h middle cerebral artery occlusion (MCAO) and 24-h reperfusion. The ischemic infarct and edema were significantly reduced in rats that received HS0608 (200 ㎎/㎏). These results suggest that the anti-oxidative properties of HS0608 may be responsible for its neuroprotective effect against focal cerebral ischemic injury and that HS0608 may have a therapeutic role in neurodegenerative diseases such as stroke.

Abstract

Previous work demonstrated that an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix markedly inhibits Aβ (25-35)-induced neurotoxicity. The present study was performed to further verify the neuroprotective effect of HS0608 on oxidative and ischemic cerebral injury using cultured rat cortical neurons and rats. Exposure of cultured cortical neurons to 100 μM hydrogen peroxide (H_2O_2) induced neuronal apoptotic death. At 10-100㎍/㎖, HS0608 inhibited neuronal death, elevation of intracellular calcium concentration ([Ca^(2+)]i), and generation of reactive oxygen species (ROS) induced by H_2O_2 in primary cultures of rat cortical neurons. In vivo, HS0608 prevented cerebral ischemic injury induced by 2-h middle cerebral artery occlusion (MCAO) and 24-h reperfusion. The ischemic infarct and edema were significantly reduced in rats that received HS0608 (200 ㎎/㎏). These results suggest that the anti-oxidative properties of HS0608 may be responsible for its neuroprotective effect against focal cerebral ischemic injury and that HS0608 may have a therapeutic role in neurodegenerative diseases such as stroke.

참고문헌 (39)

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관련 논문AI추천

Ethanol Extract of Three Plants of Curcuma longae Radix, Phellinus linteus, and Scutellariae Radix Inhibits Amyloid β Protein (25-35)-Induced Neurotoxicity in Cultured Neurons and Memory Impairment in Mice김주연, 반주연, 배기환, 성연희, 유재국, 정하연한국약용작물학회 · 한국약용작물학회지2009 · 제17권 제6호Methanol Extract of Paeonia Japonica Root Protects Cultured Rat CorticalNeurons Against Oxidative Damage Induced by Hydrogen Peroxide성연희한국약용작물학회 · 한국약용작물학회지2006 · 제14권 제2호Protective Effect of Sanguisorba officinalis L. Root onAmyloid β Protein (25-35)-induced Neuronal CellDamage in Cultured Rat Cortical Neuron배기환, 성연희, 송경식, Ju Yeon Ban, Soon Ock Cho, So Young Jeon한국약용작물학회 · 한국약용작물학회지2005 · 제13권 제5호Protective Effect of Vitis amurensis Stems and Leaves Extract on Hydrogen Peroxide-induced Oxidative Neuronal Cell Damage in Cultured Neurons김주연, 반주연, 배기환, 성연희, 송경식, 주현수한국약용작물학회 · 한국약용작물학회지2009 · 제17권 제1호Inhibitory Effect of an Ethanol Extract Mixture of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix on Amyloid β Protein (25-35)-Induced Neurotoxicity성연희, 장지연한국약용작물학회 · 한국약용작물학회지2014 · 제22권 제2호Neuroprotective Effect of Korean Mistletoe Extract against DamageInduced by Hydrogen Peroxide in Cultured Rat Cortical Neurons성연희, Ju Hyun Lee한국약용작물학회 · 한국약용작물학회지2007 · 제15권 제2호

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