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Protective Effect of Vitis amurensis Stems and Leaves Extract on Hydrogen Peroxide-induced Oxidative Neuronal Cell Damage in Cultured Neurons

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Vitis amurensis (VA; Vitaceae) has long been used in oriental herbal medicine. It has been reported that roots and seeds of VA have anti-inflammatory and antioxidant effects. In the present study, the protective effect of ethanol extract from stems and leaves of VA on hydrogen peroxide (H2O2) (100 μM)-induced neuronal cell damage was examined in primary cultured rat cortical neurons. VA (10-100 μg/ml) concentration-dependently inhibited H2O2-induced apoptotic neuronal cell death measured by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. VA inhibited H2O2-induced elevation of intracellular Ca2+ concentration ([Ca2+]i) and generation of reactive oxygen species (ROS), which were measured by fluorescent dyes. Pretreatment of VA also prevented glutamate release into medium induced by 100 μM H2O2, which was measured by HPLC. These results suggest that VA showed a neuroprotective effect on H2O2-induced neuronal cell death by interfering with H2O2-induced elevation of [Ca2+]i, glutamate release, and ROS generation. This has a significant meaning of finding a new pharmacological activity of stems and leaves of VA in the CNS.

Abstract

Vitis amurensis (VA; Vitaceae) has long been used in oriental herbal medicine. It has been reported that roots and seeds of VA have anti-inflammatory and antioxidant effects. In the present study, the protective effect of ethanol extract from stems and leaves of VA on hydrogen peroxide (H2O2) (100 μM)-induced neuronal cell damage was examined in primary cultured rat cortical neurons. VA (10-100 μg/ml) concentration-dependently inhibited H2O2-induced apoptotic neuronal cell death measured by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. VA inhibited H2O2-induced elevation of intracellular Ca2+ concentration ([Ca2+]i) and generation of reactive oxygen species (ROS), which were measured by fluorescent dyes. Pretreatment of VA also prevented glutamate release into medium induced by 100 μM H2O2, which was measured by HPLC. These results suggest that VA showed a neuroprotective effect on H2O2-induced neuronal cell death by interfering with H2O2-induced elevation of [Ca2+]i, glutamate release, and ROS generation. This has a significant meaning of finding a new pharmacological activity of stems and leaves of VA in the CNS.

참고문헌 (46)

  1. Alexi T, "Neuroprotective strategies for basal ganglia degeneration: Parkinson’s and Huntington’s diseases", Progress in Neurobiology, 60, pp.409~470 (2000)
  2. Anderson T, "Grape seed proanthocyanidin extract attenuates oxidant injury in cardiomyocytes", Pharmacological Research, 47, pp.463~469 (2003)
  3. Angelo C, "Red wine, but port wine, protects rat hippocampal dentate gyrus against ethanol-induced neuronal damage-relevance of the sugar content", Alcohol & Alcoholism, 43, pp.408~415 (2008)
  4. Bagchi D, "Molecular mechanisms of cardio- protection by a novel grape seed proanthocyanidin extract", Mutation Research, 523, pp.87~97 (2003)
  5. Yeon Hee Seong, "Protective Effect of Sanguisorba officinalis L. Root onAmyloid β Protein (25-35)-induced Neuronal CellDamage in Cultured Rat Cortical Neuron", 한국약용작물학회지, 13(5), pp.219~226 (2005), 한국약용작물학회
  6. Bao F, "Hydroxyl radicals generated in the rat spinal cord at the level produced by impact injury induce cell death by necrosis and apoptosis: protection by a metalloporphyrin", Neuroscience, 126, pp.285~295 (2004)
  7. Behl C, "Hydrogen peroxide mediates amyloid beta protein toxicity", Cell, 77, pp.817~827 (1994)
  8. Behl C, "Alzheimer’s disease and oxidative stress: implications for novel therapeutic approaches", Progress in Neurobiology, 57, pp.301~323 (1999)
  9. Butterfield DA, "Evidence of oxidative damage in Alzheimer's disease brain: central role for amyloid beta-peptide", Trends in Molecular Medicine, 7, pp.548~554 (2001)
  10. Camacho A, "Role of glutamate transporters in the clearance and release of glutamate during ischemia and its relation to neuronal death", Archives of Medical Research, 37, pp.11~18 (2006)
  11. Chan PH, "Reactive oxygen radicals in signaling and damage in the ischemic brain", Journal of Cerebral Blood Flow and Metabolism, 21, pp.2~14 (2001)
  12. Chen L, "MAPK and mTOR pathways are involved in cadmium-induced neuronal apoptosis", Journal of Neurochemistry, 105, pp.251~261 (2008)
  13. Hyang Sook Chun, "Neuroprotective Effects of Several Korean Medicinal Plants Traditionally Used for Stroke Remedy", Journal of Medicinal Food, 11(2), pp.246~251 (2008), 한국식품영양과학회
  14. Crack PJ, "Reactive oxygen species and the modulation of stroke", Free Radical Biology and Medicine, 38, pp.1433~1444 (2005)
  15. Das A, "Cytoprotective effects of melatonin on C6 astroglial cells exposed to glutamate excitotoxicity and oxidative stress", Journal of Pineal Research, 45, pp.117~124 (2008)
  16. Duffy S, "In vitro ischemia promotes calcium influx and intracellular calcium release in hippocampal astrocytes", Journal of Neuroscience, 16, pp.71~81 (1996)
  17. Ekinci FJ, "β-Amyloid-induced calcium influx induces apoptosis in culture by oxidative stress rather than tau phosphorylation", Molecular Brain Research, 76, pp.389~395 (2000)
  18. Fatokun AA, "Cell death in rat cerebellar granule neurons induced by hydrogen peroxide in vitro: Mechanisms and protection by adenosine receptor ligands", Brain Research, 1132, pp.193~202 (2007)
  19. Fu Y, "EGCG protects HT-22 cells against glutamate-induced oxidative stress", Neurotoxicity Research, 10, pp.23~30 (2006)
  20. Gardner AM, "Apoptotic vs. nonapoptotic cytotoxicity induced by hydrogen peroxide", Free Radical Biology & Medicine, 22, pp.73~83 (1997)
  21. Halliwell B, "Oxidative stress and neurodegeneration: where are we now?", Journal of Neurochemistry, 97, pp.1634~1658 (2006)
  22. Huang SS, "Resveratrol reduction of infarct size in Long Evans rats subjected to focal cerebral ischemia", Life Sciences, 69, pp.1057~1065 (2001)
  23. Jang MH, "Resveratrol oligomers from Vitis amurensis attenuate beta-amyloid-induced oxidative stress in PC12 cells", Biological & Pharmacological Bulletin, 30, pp.1130~1134 (2007)
  24. Jang MH, "Inhibition of cholinesterase and amyloid-beta aggregation by resveratrol oligomers from Vitis amurensis", Phytotherapy Research, 22, pp.544~549 (2008)
  25. Jellinger KA, "Cell death mechanisms in Parkinson’s disease", Journal of Neural Transmission, 107, pp.1~29 (2000)
  26. Kim SD, "Identification of ASK1, MKK4, JNK, c-Jun, and caspase-3 as a signaling cascade involved in cadmium-induced neuronal cell apoptosis", Biochemical and Biophysical Research Communi-cations, 328, pp.326~334 (2005)
  27. Lee EO, "Potent inhibition of Lewis lung cancer growth by heyneanol A from the roots of Vitis amurensis through apoptotic and anti-angiogenic activities", Carcinogenesis, 27, pp.2059~2069 (2006)
  28. Ju Hyun Lee, "Neuroprotective Effect of Korean Mistletoe Extract against DamageInduced by Hydrogen Peroxide in Cultured Rat Cortical Neurons", 한국약용작물학회지, 15(2), pp.105~111 (2007), 한국약용작물학회
  29. 이순복, "과산화수소수로 유도된 배양신경세포손상에 대한 참죽나무잎 추출물의 보호효과", 한국약용작물학회지, 15(6), pp.444~450 (2007), 한국약용작물학회
  30. Mailly F, "Increase in external glutamate and NMDA receptor activation contribute to H O -induced neuronal apoptosis", Journal of Neurochemistry, 73, pp.1181~1188 (1999)
  31. Mizutani K, "Protective effect of resveratrol on oxidative damage in male and female stroke-prone spontaneously hypertensive rats", Clinical and experimental pharmacology & physiology, 28, pp.55~59 (2001)
  32. Nassiri-Asl M, "Review of the pharmacological effects of Vitis vinifera (Grape) and its bioactive compounds" (2009), Phytotherapy research
  33. Numakawa Y, "17β-Estradiol protects cortical neurons against oxidative stress-induced cell death through reduction in the activity of mitogen-activated protein kinase and in the accumulation of intracellular calcium", Endocrinology, 148, pp.627~637 (2007)
  34. Yeon Hee Seong, "Methanol Extract of Paeonia Japonica Root Protects Cultured Rat CorticalNeurons Against Oxidative Damage Induced by Hydrogen Peroxide", 한국약용작물학회지, 14(2), pp.70~76 (2006), 한국약용작물학회
  35. Privat C, "Antioxidant properties of tran-epsilon-viniferin as compared to stilbene derivatives in aqueous and nonaqueous media", Journal of Agricultural and Food Chemistry, 50, pp.1213~1217 (2002)
  36. Quiney C, "Flavones and polyphenol inhibit the NO pathway during apoptosis of leukemia B-cells", Leukemia Research, 28, pp.851~861 (2004)
  37. Sato M, "Cardioprotective effects of grape seed proanthocyanidin against ischemic reperfusion injury", Journal of Molecular and Cellular Cardiology, 31, pp.1289~1297 (1999)
  38. Shao ZH, "Grape seed proanthocyanidins induce pro-oxidant toxicity in cardiomyocytes", Cardiovascular Toxicology, 3, pp.331~339 (2003)
  39. Su JYC, "Reduction of H O - evoked intracellular calcium increases in the rat N18-RE-105 neuronal cell line by pretreatment with an electrophilic antioxidant inducer", Neuroscience Letter, 273, pp.109~112 (1999)
  40. Vauzour D, "Champagne wine polyphenols protect primary cortical neurons against peroxynitrite-induced injury", Journal of Agricultural and Food Chemistry, 55, pp.2854~2860 (2007)
  41. Vitseva O, "Grape seed and skin extracts inhibit platelet function and release of reactive oxygen intermediates", Journal of Cardiovascular Pharmacology, 46, pp.445~451 (2005)
  42. Volterra A, "Glutamate uptake inhibition by oxygen free radicals in rat cortical astrocytes", Journal of Neuroscience, 14, pp.2924~2932 (1994)
  43. Wang H, "Mechanisms of hydrogen peroxide-induced calcium dysregulation in PC12 cells", Free Radical Biology and Medicine, 28, pp.1222~1231 (2000)
  44. Wang J, "Grape-derived polyphenolics prevent Aβ oligomerization and attenuate cognitive deterioration in a mouse model of Alzheimer’s disease", Journal of Neuroscience, 28, pp.6388~6392 (2008)
  45. Wang J, "Moderate consumption of Cabernet Sauvignon attenuates A-beta neuropathology in a mouse model of Alzheimer’s disease", FASEB Journal, 20, pp.2313~2320 (2006)
  46. Wang Q, "Dietary grape supplement ameliorates cerebral ischemia-induced neuronal death in gerbils", Molecular nutrition & Food Research, 49, pp.443~451 (2005)

관련 논문AI추천

Neuroprotective Effect of Korean Mistletoe Extract against DamageInduced by Hydrogen Peroxide in Cultured Rat Cortical Neurons성연희, Ju Hyun Lee한국약용작물학회 · 한국약용작물학회지2007 · 제15권 제2호과산화수소수로 유도된 배양신경세포손상에 대한 참죽나무잎 추출물의 보호효과김주연, 반주연, 배기환, 성연희, 이순복, 조순옥, 주현수한국약용작물학회 · 한국약용작물학회지2007 · 제15권 제6호Methanol Extract of Paeonia Japonica Root Protects Cultured Rat CorticalNeurons Against Oxidative Damage Induced by Hydrogen Peroxide성연희한국약용작물학회 · 한국약용작물학회지2006 · 제14권 제2호Inhibitory Effect of an Ethanol Extract Mixture of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix on Amyloid β Protein (25-35)-Induced Neurotoxicity성연희, 장지연한국약용작물학회 · 한국약용작물학회지2014 · 제22권 제2호Protective Effect of Sanguisorba officinalis L. Root onAmyloid β Protein (25-35)-induced Neuronal CellDamage in Cultured Rat Cortical Neuron배기환, 성연희, 송경식, Ju Yeon Ban, Soon Ock Cho, So Young Jeon한국약용작물학회 · 한국약용작물학회지2005 · 제13권 제5호Moutan Cortex Extract Inhibits Amyloid β Protein (25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons김주연, 반주연, 성연희, 송경식, 주현수한국약용작물학회 · 한국약용작물학회지2008 · 제16권 제6호

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