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Protective Effect of Sanguisorba officinalis L. Root onAmyloid β Protein (25-35)-induced Neuronal CellDamage in Cultured Rat Cortical Neuron

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초록

Sanguisorbae radix (SR) from Sanguisorba officinalis L. (Losaceae) is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of SR on amyloid β protein (25-35) (Aβ (25-35)), a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. SR, over a concentration range of 10-50㎍/㎖, inhibited the Aβ (25-35) (10 μM)-induced neuronal cell death, as assessed by a 3- [4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. Pretreatment of SR (50㎍/㎖) inhibited 10 μM Aβ (25-35)-induced elevation of cytosolic calcium concentration ([Ca2+]C), which was measured by a fluorescent dye, fluo-4 AM. SR (10 and 50㎍/㎖) inhibited glutamate release into medium induced by 10 μM Aβ (25-35), which was measured by HPLC, and generation of reactive oxygen species. These results suggest that SR prevents Aβ (25-35)-induced neuronal cell damage in vitro.

Abstract

Sanguisorbae radix (SR) from Sanguisorba officinalis L. (Losaceae) is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of SR on amyloid β protein (25-35) (Aβ (25-35)), a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. SR, over a concentration range of 10-50㎍/㎖, inhibited the Aβ (25-35) (10 μM)-induced neuronal cell death, as assessed by a 3- [4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. Pretreatment of SR (50㎍/㎖) inhibited 10 μM Aβ (25-35)-induced elevation of cytosolic calcium concentration ([Ca2+]C), which was measured by a fluorescent dye, fluo-4 AM. SR (10 and 50㎍/㎖) inhibited glutamate release into medium induced by 10 μM Aβ (25-35), which was measured by HPLC, and generation of reactive oxygen species. These results suggest that SR prevents Aβ (25-35)-induced neuronal cell damage in vitro.

참고문헌 (32)

  1. "β-Amyloid peptide fragment25-35 potentiates the calcium-dependent release of excitatoryamino acids from depolarized hippocampal slices", 41, pp.561~566 (1995)
  2. "Blockade of 5-HT3 receptor withMDL72222 and Y25130 reduces amyloid β protein -induced neurotoxicity in cultured rat cortical neurons" (2005)
  3. "Induction ofcytochrome c-mediated apoptosis by amyloid β 25-35 requiresfunctional mitochondria", 931, pp.117~125 (2002)
  4. "Neuropathology ofsynthetic β-amyloid peptide analogs in vivo", pp.44~50 (1994)
  5. "Pomolic acid derivatives from theroot of Sanguisorba officinalis", pp.1317~1320 (1992)
  6. "a discussion of in vitro findings", pp.587~590 (1992)
  7. "β-Amyloid-inducedcalcium influx induces apoptosis in culture by oxidativestress rather than tau phosphorylation", 76, pp.389~395 (2000)
  8. "Activation of the L voltage-sensitive calcium channel by mitogen-activated protein kinase following exposure of neuronal cells to betaamyloid", 274, pp.30322~30327 (1999)
  9. "Amino acid neurotransmittersin postmortem human brain analyzed by highperformance liquid chromatography with electrochemicaldetection", pp.305~19 315 (1987)
  10. "Determination of intracellularCa2+ concentration can be useful tool to predict neuronal damageand neuroprotective properties of drugs", 732, pp.87~94 (1996)
  11. "Secreted forms of β-amyloidprecursor protein protect hippocampal neurons against amyloidβ-peptide-induced oxidative injury", pp.1~12 (1994)
  12. "Neurodegeneration mediated by glutamate and peptide: a comparison and possible interaction", 691, pp.169~179 (1995)
  13. "Neuroprotective approaches in experimental models of β-amyloid neurotoxicity : relevance to Alzheimer's disease", 23, pp.963~1008 (1999)
  14. "Accelerated Alzheimer-type phenotype in transgenicmice carrying both mutant amyloid precursor proteinand presenilin 1 transgenes", 4, pp.97~100 (1998)
  15. "Disturbances ofcalcium homeostasis in ischemic stroke", 11, pp.373~386 (1999)
  16. "Age-related CNS disorderand early death in transgenic FVB", N mice overexpressingAlzheimer amyloid precursor proteins, 1995, pp.15, 1203~1208
  17. "The pharmacology of Chinese herbs", pp.155~ (1999), CRCpress LLC
  18. "The toxicity in vitro of β-amyloid protein", 311, pp.1~16 (1995)
  19. "Multiplepathways of apoptosis in PC12 cells CrmA inhibits apoptosisinduced by beta-amyloid", 274, pp.2107~2112 (1999)
  20. "β-Amyloid proteinincreases the vulnerability of cultured neurons to excitotoxicdamage", pp.315~320 (1990)
  21. "Chronic stimulation ofGABAA receptor with muscimol reduces amyloid β protein -induced neurotoxicity in cultured rat cortical cells", 52, pp.347~356 (2005)
  22. "inhibitors from Sanguisorbae Radix", 28, pp.799~803 (2005)
  23. "Phosphatidic acid-induced elevation of intracellularCa2+ is mediated RhoA and H2O2 in rat-2 fibroblasts", 273, pp.12710~12715 (1998)
  24. "NMDA receptor antagonists disinhibit rat posterior cingulate and retrosplenial cortices: a potential mechanism of neu×toxicity", 22, pp.3070~3080 (2002)
  25. "The role of oxidative stressin the toxicity induced by amyloid β-peptide in Alzheimer'sdisease", 62, pp.633~648 (2000)
  26. "A comparative study of DAPI and Hoechast 33258 and 33342 as chromosomal DNA stains", pp.7~11 (1985)
  27. "Oxidative glutamate toxicityinvolves mitochondrial dysfunction and perturbation of intracellularCa2+ homeostasis", 37, pp.227~236 (2000)
  28. "Anti-allergic effects of Sanguisorbaofficinalis on animal models of allergic reactions", 24, pp.455~468 (2002)
  29. "Neuroprotective approaches in experimentalmodels of β-amyloid neurotoxicity relevance to Alzheimer'sdisease", 23, pp.963~1008 (1999)
  30. "neurotoxic properties of aggregatedamyloid β protein", pp.240~244 (1994)
  31. "Amyloid β protein potentiates Ca2+ influx through L-type voltage sensitive Ca2+ channels", 68, pp.265~271 (1997)
  32. "β-Amyloid peptidefragment 31-35 induces apoptosis in cultured cortical neurons", 92, pp.177~184 (1999)

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