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Inhibitory Effect of an Ethanol Extract Mixture of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix on Amyloid β Protein (25-35)-Induced Neurotoxicity

장지연|성연희
한국약용작물학회|2014.04|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제22권 제2호|pp. 105-112|DOI http://dx.doi.org/10.7783/KJMCS.2014.22.2.105

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키워드

Vitis amurensisAralia cordataGlycyrrhizae radixAmyloid β ProteinCultured NeuronsMemory Impairm

초록

The present study investigated an ethanol extract (SSB) of a mixture of three medicinal plants of Vitisamurensis, Aralia cordata, and Glycyrrhizae radix for possible neuroprotective effects on neurotoxicity induced byAmyloid β protein (Aβ) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured corticalneurons to 15 μM Aβ (25-35) for 36 h induced neuronal apoptotic death. At 1-30㎍/㎖, SSB inhibited neuronal death,elevation of intracellular calcium concentration ([Ca2+]i), and generation of reactive oxygen species (ROS) induced by Aβ(25-35) in cultured cortical neurons. Memory impairment and increase of acetylcholinesterase activity induced by intracerebroventricularinjection of mice with 16 nmol Aβ (25-35) was inhibited by chronic treatment with SSB (25, 50 and100 ㎎/㎏, p.o., for 8 days). From these results, it is suggested that antidementia effect of SSB is due to its neuroprotectiveeffect against Aβ (25-35)-induced neurotoxicity and that SSB may have a therapeutic role in preventing the progression ofAlzheimer’s disease.

Abstract

The present study investigated an ethanol extract (SSB) of a mixture of three medicinal plants of Vitisamurensis, Aralia cordata, and Glycyrrhizae radix for possible neuroprotective effects on neurotoxicity induced byAmyloid β protein (Aβ) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured corticalneurons to 15 μM Aβ (25-35) for 36 h induced neuronal apoptotic death. At 1-30㎍/㎖, SSB inhibited neuronal death,elevation of intracellular calcium concentration ([Ca2+]i), and generation of reactive oxygen species (ROS) induced by Aβ(25-35) in cultured cortical neurons. Memory impairment and increase of acetylcholinesterase activity induced by intracerebroventricularinjection of mice with 16 nmol Aβ (25-35) was inhibited by chronic treatment with SSB (25, 50 and100 ㎎/㎏, p.o., for 8 days). From these results, it is suggested that antidementia effect of SSB is due to its neuroprotectiveeffect against Aβ (25-35)-induced neurotoxicity and that SSB may have a therapeutic role in preventing the progression ofAlzheimer’s disease.

참고문헌 (43)

  1. Alkam T, "A natural scavenger of peroxynitrites, rosmarinic acid, protects against impairment of memory induced by Abeta(25-35)", Behavioural Brain Research, 180, pp.139~145 (2007)
  2. Atack JR, "Molecular forms of acetylcholinesterase in senile dementia of Alzheimer type : Selective loss of the intermediate(10S)form", Neuroscience Letters, 40, pp.199~204 (1983)
  3. Bae K., "The medicinal plants of Korea", pp.362~363 (2000), Kyo-Hak Publishing Co
  4. Ban JY, "Catechin and epicatechin from Smilacis chinae rhizome protect cultured rat cortical neurons against amyloid beta protein(25-35)-induced neurotoxicity through inhibition of cytosolic calcium elevation", Life Sciences, 79, pp.2251~2259 (2006)
  5. Butterfield DA, "Evidence of oxidative damage in Alzheimer's disease brain : Central role for amyloid beta-peptide", Trends in Molecular Medicine, 7, pp.548~554 (2001)
  6. Chen CY, "Attenuation of abeta-induced apoptosis of plant extract(Saengshik)mediated by the inhibition of mitochondrial dysfunction and antioxidative effect", Annals of the New York Academy of Sciences, 1095, pp.399~411 (2007)
  7. Cho SO, "Aralia cordata protects against amyloid beta protein(25-35)-induced neurotoxicity in cultured neurons and has antidementia activities in mice", Journal of Pharmacological Sciences, 111, pp.22~32 (2009)
  8. 정명근, "맥문동 종실 안토시아닌 분획물의 멜라닌 생성 억제 및 미백 효과", 한국약용작물학회지, 21(5), pp.361~371 (2013), 한국약용작물학회
  9. Nguyen Hai Dang, "Inhibitory Constituents against Cyclooxygenases from Aralia cordata Thunb", Archives of Pharmacal Research, 28(1), pp.28~33 (2005), 대한약학회
  10. Du Y, "Oleanolic acid protects against myocardial ischemia-reperfusion injury by enhancing mitochondrial antioxidant mechanism mediated by glutathione and alpha-tocopherol in rats", Planta Medica, 72, pp.222~227 (2006)
  11. Ekinci FJ, "Beta-amyloidinduced calcium influx induces apoptosis in culture by oxidative stress rather than tau phosphorylation", Brain Research, 76, pp.389~395 (2000)
  12. Gasparini L, "Non-steroidal antiinflammatory drugs(NSAIDs)in Alzheimer’s disease : Old and new mechanisms of action", Journal of Neurochemistry, 91, pp.521~536 (2004)
  13. Gitter BD, "Amyloid beta peptide potentiates cytokine secretion by interleukin-1beta-activated human astrocytoma cells", Proceedings of the National Academy of Sciences of the United States of America, 92, pp.10738~10741 (1995)
  14. Gray CW, "Neurodegeneration mediated by glutamate and beta-amyloid peptide : A comparison and possible interaction", Brain Research, 691, pp.169~179 (1995)
  15. Ha DT, "stillbenes and oligostilbenes from leaf and stem of Vitis amurensis and their cytotoxic activity", Archives of Pharmacal Research, 32, pp.177~183 (2009)
  16. Ha DT, "Antioxidant and lipoxygenase inhibitory activity of oligostilbenes from the leaf and stem of Vitis amurensis", Journal of Ethnopharmacology, 125, pp.304~309 (2009)
  17. Han B, "Studies on the antiinflammatory activity of Aralia continentalis(III)", Archives of Pharmacal Research, 8, pp.59~65 (1985)
  18. Huang KC., "The pharmacology of Chinese herbs", pp.364~368 (1999), CRC press LLC
  19. Ivins KJ, "Multiple pathways of apoptosis in PC12 cells. CrmA inhibits apoptosis induced by beta-amyloid", The Journal of Biological Chemistry, 274, pp.2107~2112 (1999)
  20. Ha Yeon Jeong, "Leaf and Stem of Vitis amurensis and Its Active Components Protect against Amyloid β Protein (25-35)-induced Neurotoxicity", Archives of Pharmacal Research, 33(10), pp.1655~1664 (2010), 대한약학회
  21. 정명환, "Inhibitory Effect of Chaenomeles sinensis Fruit on Amyloid β Protein (25-35)-Induced Neurotoxicity in Cultured Neurons and Memory Impairment in Mice", 한국약용작물학회지, 20(1), pp.8~15 (2012), 한국약용작물학회
  22. 김주연, "Protective Effect of an Ethanol Extract Mixture of Curcuma longae Radix, Phellinus linteus, and Scutellariae Radix on Oxidative Neuronal Damage", 한국약용작물학회지, 19(1), pp.31~37 (2011), 한국약용작물학회
  23. Koh JY, "Beta-amyloid protein increases the vulnerability of cultured cortical neurons to excitotoxic damage", Brain Research, 533, pp.315~320 (1990)
  24. Kontush A., "Amyloid-beta : an antioxidant that becomes a pro-oxidant and critically contributes to Alzheimer’s disease", Free radical Biology & Medicine, 31, pp.1120~1131 (2001)
  25. Kumar S, "Isoliquiritigenin inhibits IkappaB kinase activity and ROS generation to block TNF-alpha induced expression of cell adhesion molecules on human endothelial cells", Biochemical Pharmacology, 73, pp.1602~1612 (2007)
  26. Lee BY, "Chronic stimulation of GABAA receptor with muscimol reduces amyloid beta protein(25-35)-induced neurotoxicity in cultured rat cortical cells", Neuroscience Research, 52, pp.347~356 (2005)
  27. 이홍규, "Inhibitory Effects of Glycyrrhizae Radix and Its Active Component, Isoliquiritigenin, on Aβ(25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons", Archives of Pharmacal Research, 35(5), pp.897~904 (2012), 대한약학회[DOI]
  28. Ik Soo Lee, "Cytotoxic and COX-2 Inhibitory Constituents from the Aerial Parts of Aralia cordata", Archives of Pharmacal Research, 29(7), pp.548~555 (2006), 대한약학회
  29. Maurice T, "Amnesia induced in mice by centrally administered beta-amyloid peptides involves cholinergic dysfunction", Brain Research, 706, pp.181~193 (1996)
  30. Miranda S, "The role of oxidative stress in the toxicity induced by amyloid beta-peptide in Alzheimer’s disease", Progress in Neurobiology, 62, pp.633~648 (2000)
  31. Morais Cardoso S, "Induction of cytochrome c-mediated apoptosis by amyloid beta 25-35 requires functional mitochondria", Brain Research, 931, pp.117~125 (2002)
  32. Park HJ, "Effects of Aralia continentalis on hyperalgesia with peripheral inflammation", Phytotherapy Research, 19, pp.511~513 (2005)
  33. Pike CJ, "Structure-activity analyses of beta-amyloid peptides : Contributions of the beta 25-35 region to aggregation and neurotoxicity", Journal of Neurochemistry, 64, pp.253~265 (1995)
  34. Rice-Evans CA, "The relative antioxidant activities of plant-derived polyphenolic flavonoids", Free Radical Research, 22, pp.375~383 (1995)
  35. Sano M, "A controlled trial of selegiline, alpha-tocopherol, or both as treatment for Alzheimer's disease. The Alzheimer’s Disease Cooperative Study", The New England Journal of Medicine, 336, pp.1216~1222 (1997)
  36. Smith MA, "Widespread peroxynitrite-mediated damage in Alzheimer’s disease", The Journal of Neuroscience, 17, pp.2653~2657 (1997)
  37. Stepanichev MY, "Single intracerebroventricular administration of amyloid-beta(25-35)peptide induces impairment in short-term rather than long-term memory in rats", Brain Research Bulletin, 61, pp.197~205 (2003)
  38. Tang SY, "Characterization of antioxidant and antiglycation properties and isolation of active ingredients from traditional chinese medicines", Free radical Biology & Medicine, 36, pp.1575~1587 (2004)
  39. Tohda C, "Abeta(25-35)-induced memory impairment, axonal atrophy, and synaptic loss are ameliorated by M1, A metabolite of protopanaxadiol-type saponins", Neuropsychopharmacology, 29, pp.860~868 (2004)
  40. Ueda K, "Amyloid beta protein potentiates Ca2+ influx through Ltype voltage-sensitive Ca2+ channels : A possible involvement of free radicals", Journal of Neurochemistry, 68, pp.265~271 (1997)
  41. Um MY, "Protective effect of Polygonum multiflorum Thunb on amyloid beta-peptide 25-35 induced cognitive deficits in mice", Journal of Ethnopharmacology, 104, pp.144~148 (2006)
  42. Wang Q, "Resveratrol protects against neurotoxicity induced by kainic acid", Neurochemical Research, 29, pp.2105~2112 (2004)
  43. Yan XZ, "Beta-amyloid peptide fragment 31-35 induces apoptosis in cultured cortical neurons", Neuroscience, 92, pp.177~184 (1999)

관련 논문AI추천

Ethanol Extract of Three Plants of Curcuma longae Radix, Phellinus linteus, and Scutellariae Radix Inhibits Amyloid β Protein (25-35)-Induced Neurotoxicity in Cultured Neurons and Memory Impairment in Mice김주연, 반주연, 배기환, 성연희, 유재국, 정하연한국약용작물학회 · 한국약용작물학회지2009 · 제17권 제6호Inhibitory Effect of Chaenomeles sinensis Fruit on Amyloid β Protein (25-35)-Induced Neurotoxicity in Cultured Neurons and Memory Impairment in Mice성연희, 송경식, 정명환한국약용작물학회 · 한국약용작물학회지2012 · 제20권 제1호Protective Effect of Sanguisorba officinalis L. Root onAmyloid β Protein (25-35)-induced Neuronal CellDamage in Cultured Rat Cortical Neuron배기환, 성연희, 송경식, Ju Yeon Ban, Soon Ock Cho, So Young Jeon한국약용작물학회 · 한국약용작물학회지2005 · 제13권 제5호Protective Effect of Vitis amurensis Stems and Leaves Extract on Hydrogen Peroxide-induced Oxidative Neuronal Cell Damage in Cultured Neurons김주연, 반주연, 배기환, 성연희, 송경식, 주현수한국약용작물학회 · 한국약용작물학회지2009 · 제17권 제1호Moutan Cortex Extract Inhibits Amyloid β Protein (25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons김주연, 반주연, 성연희, 송경식, 주현수한국약용작물학회 · 한국약용작물학회지2008 · 제16권 제6호Protection of Amyloid β Protein (25-35)-induced Neuronal Cell Damage by Methanol Extract of New Stem of Phyllostachys nigra Munro var. henonis Stapf in Cultured Rat Cortical Neuron권순호, 김진배, 박주연, 배기환, 성낙술, 성연희, 송경식, 조순옥한국약용작물학회 · 한국약용작물학회지2005 · 제13권 제2호

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