쥐오줌풀의 항산화 및 산화적 DNA 손상 억제 활성
Antioxidative Activities and Inhibition Effects on Oxidative DNA Damage of Valeriana fauriei
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Background: Valeriana fauriei (Valerianaceae) has been used to as a traditional medicine to treat a variety of symptoms, including headache, insomnia, hypertension, and menstrual irregularity. However, the present study investigates the species' antioxidant activity and its inhibition of oxidative DNA damage, which have yet to be studied. Methods and Results: The antioxidant activity was assessed using radical scavenging assays with 1,1-diphenyl-2-picryl hydrazyl (DPPH) and, 2, 2'-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) and a reducing power assay. The total phenol content was also analyzed, and phenolic compounds were detected using HPLC/UV, whereas the inhibitory effect of Valeriana fauriei on oxidative DNA damage was measured using φ-174 RF I plasmid DNA cleavage assay. The DPPH and ABTS radical scavenging activity were 75.17 ± 3.55% and 95.83 ± 0.63%, repectively, and the reducing power was 93.14 ± 1.74 at 200 ㎍/㎖. The total phenol content was 10.24 ± 0.04㎎/g, whereas chlorogenic acid, catechin, caffeic acid and epicatechin were identified using HPLC/UV, and the φ-174 RF I plasmid DNA cleavage assay indicated that V. fauriei provided protection against oxidative damage. Conclusions: The results of the present study suggest that V. fauriei has powerful antioxidant activity that can provide protective effects against the oxidative DNA damage caused by free radicals. The species, therefore, provides a valuable resource for the development of natural pharmaceutical to treat aging, cancer, and degenerative diseases.
Abstract
Background: Valeriana fauriei (Valerianaceae) has been used to as a traditional medicine to treat a variety of symptoms, including headache, insomnia, hypertension, and menstrual irregularity. However, the present study investigates the species' antioxidant activity and its inhibition of oxidative DNA damage, which have yet to be studied. Methods and Results: The antioxidant activity was assessed using radical scavenging assays with 1,1-diphenyl-2-picryl hydrazyl (DPPH) and, 2, 2'-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) and a reducing power assay. The total phenol content was also analyzed, and phenolic compounds were detected using HPLC/UV, whereas the inhibitory effect of Valeriana fauriei on oxidative DNA damage was measured using φ-174 RF I plasmid DNA cleavage assay. The DPPH and ABTS radical scavenging activity were 75.17 ± 3.55% and 95.83 ± 0.63%, repectively, and the reducing power was 93.14 ± 1.74 at 200 ㎍/㎖. The total phenol content was 10.24 ± 0.04㎎/g, whereas chlorogenic acid, catechin, caffeic acid and epicatechin were identified using HPLC/UV, and the φ-174 RF I plasmid DNA cleavage assay indicated that V. fauriei provided protection against oxidative damage. Conclusions: The results of the present study suggest that V. fauriei has powerful antioxidant activity that can provide protective effects against the oxidative DNA damage caused by free radicals. The species, therefore, provides a valuable resource for the development of natural pharmaceutical to treat aging, cancer, and degenerative diseases.
참고문헌 (35)
- 안정좌, "Effects of Abeliophyllum distichum Nakai Flower Extracts on Antioxidative Activities and Inhibition of DNA Damage", 한국자원식물학회지, 26(3), pp.355~361 (2013), 한국자원식물학회[DOI]
- Ames BN, "Dietary carcinogens and anticarcinogens:Oxygen radical and degenerative diseases", Risk Analysis in the Private Sector, 220, pp.297~321 (1985)
- Association of Official Analytical Chemists, "Official methods of analysis", Association of Official Analytical Chemists, 8~35 (1984)
- Becker H, "Valepotriates in Valeriana thalictroides", Planta medica, 49, pp.64~64 (1983)
- Bondet V, "Kinetics and mechanisms of antioxidant activity using the DPPH free radical method", LWT-Food Science and Technology, 30, pp.609~615 (1997)
- Bors W, "Radical chemistry of flavonoid antioxidants", Advances in Experimental Medicine and Biology, 264, pp.165~170 (1990)
- Chanda S, "In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties : An overview", African Journal of Microbiology Research, 3, pp.981~996 (2009)
- Cho CH, "Differences of major compounds in Valerian fauriei var. dasycarpa HARA and Valeriana officinalis L. grown at different places in Korea", Korean Journal of Medicinal Crop Science, 4, pp.109~113 (1996)
- Choe SY, "Toxicological studies of antioxidants butylated hydroxytoluene(BHT) and butylated hydroxyanisole(BHA)", Korean Journal of Food Science and Technology, 14, pp.283~288 (1982)
- Choi YH, "Differences of essential oil content in Valeriana fauriei var. dasycarpa Hara, V. officinalis var lalfolia Miq and V. wallichii DC", Korean Journal of Medicinal Crop Science, 3, pp.217~225 (1995)
- Cuvelier ME, "Comparison of the antioxidative activity of some acid-phenols: Structureactivity relationship", Bioscience, Biotechnology, and Biochemistry, 56, pp.324~325 (1992)
- Dugaheh MA, "Antioxidant effect and study of bioactive components of Valeriana sisymbriifolia and Nardostachys jatamansii in comparison to Valeriana officinalis", Pakistan Journal of Pharmaceutical Sciences, 26, pp.53~58 (2013)
- Ferreira IC, "Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal : Individual cap and stipe activity", Food Chemistry, 100, pp.1511~1516 (2007)
- Ferreres F, "Improved loquat(Eriobotrya japonica Lindl. )cultivars : Variation of phenolics and antioxidative potential", Food Chemistry, 114, pp.1019~1027 (2009)
- Halliwell B., "Antioxidant characterization: Methodology and mechanism", Biochemical Pharmacology, 49, pp.1341~1348 (1995)
- Houghton PJ, "The biological activity of valerian and related plants", Journal of Ethnopharmacology, 22, pp.121~142 (1988)
- Huang MT, "Phenolic compounds in food and their effects on health II. Antioxidants and cancer prevention", pp.54~71 (1992), American Chemical Society
- 김홍실, "쥐오줌풀 부정근으로부터 valepotriates와 valerenic acid 생산에 미치는 elicitor의 효과", 한국약용작물학회지, 15(4), pp.241~245 (2007), 한국약용작물학회
- Johnson TM, "Reactive oxygen species are downstream mediators of p53-dependent apoptosis", Proceedings of the National Academy of Sciences, 93, pp.11848~11852 (1996)
- 정성제, "약용 식물 추출물의 항산화 활성 검색", Applied Biological Chemistry, 47(1), pp.135~140 (2004), 한국응용생명화학회
- Jung YJ, "Oxidative DNA damage and cytotoxicity induced by copper-stimulated redox cycling of salsolinol, a neurotoxic tetrahydroisoquinoline alkaloid", Free Radical Biology and Medicine, 30, pp.1407~1417 (2001)
- Kawanishi S, "Mechanism of guanine-specific DNA damage by oxidative stress and its role in carcinogenesis and aging", Mutation Research, 488, pp.65~76 (2001)
- Lawrence BM, "A review of the world production of essential oils", Perfumer and Flavorist, 10, pp.1~16 (1985)
- 김남우, "민자주방망이버섯의 폴리페놀 함량과 항산화활성", 한국식품영양과학회지, 35(10), pp.1309~1314 (2006), 한국식품영양과학회
- Marnett LJ, "Oxyradicals and DNA damage", Carcinogenesis, 21, pp.361~370 (2000)
- Meir S, "Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves", Journal of Agricultural Food and Chemistry, 43, pp.1813~1819 (1995)
- Navarrete A, "Chemical fingerprinting of Valeriana species : Simultaneous determination of valerenic acids, flavonoids, and phenylpropanoids using liquid chromatography with ultraviolet detection", Journal of AOAC International, 89, pp.8~15 (2006)
- Oyaizu M., "Studies on products of browning reaction:Antioxidative activities of products of browning reaction prepared from glucosamine", Japanese Journal of Nutrition, 44, pp.307~315 (1986)
- Padayatty SJ, "Vitamin C as an antioxidant : Evaluation of its role in disease prevention", Journal of the American College of Nutrition, 22, pp.18~35 (2003)
- 박은비, "산수유 추출방법에 따른 항산화 기능 분석", 생명과학회지, 22(11), pp.1507~1514 (2012), 한국생명과학회[DOI]
- Que F, "Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds", Food Research International, 39, pp.581~587 (2006)
- Seifried HE, "A review of the interaction among dietary antioxidants and reactive oxygen species", The Journal of Nutritional Biochemistry, 18, pp.567~579 (2007)
- Surh YJ, "Molecular mechanisms of chemopreventive effects of selected dietary and medicinal phenolic substances", Mutation Research, 428, pp.305~327 (1999)
- Van den Berg R, "Applicability of an improved trolox equivalent antioxidant capacity(TEAC)assay for evaluation of antioxidant capacity measurements of mixtures", Food Chemistry, 66, pp.511~517 (1999)
- Wills RBH, "Changes in valerenic acids content of valerian root(Valeriana officinalis L. )during longterm storage", Food Chemistry, 115, pp.250~253 (2009)