염류내성관련 SAL1 유전자에 의한 인삼 형질전환
Transformation of Korean Ginseng (Panax ginseng C.A. Meyer)with Salt Toleranc SAL1 Gene
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Abstract
Salt-tolerant transgenic Panax ginseng plants were produced by introducing the SAL1 gene (3’(2’), 5’-bis-phosphate nucleotidase) that confers tolerance to the salts through Agrobacterium tumefaciens co-cultivation. Cotyledon explants of immature ginseng zygotic embryos cultured on Murashige and Skoog medium lacking growth regulators formed somatic embryos directly with below 10%, but the 74% tranformation rate were observed at the treatment of phytohormone with 1.0 / 2,4-D and 0.5 / kinetin. Somatic embryos were initially cultured on MS medium supplemented with 250 / cefotaxime for 3 weeks and subsequently subcultured five times to a medium containing 100 / kanamycin and 250 / cefotaxime. Upon development into the cotyledonary stage, these somatic embryos were transferred to on the medium containing 50 / kanamycin and 10 / gibberellic acid to induce germination and strong selection. Integration of the transgene into the plants was confirmed by polymerase chain reaction with specific primers. The ginseng transformants with well-developed shoots and roots were successfully acclimatized in a greenhouse when they were planted in soil.
참고문헌 (14)
- "Salttolerance in Lycopersicon species Character definition andchanges in gene expression", pp.737~743 (1993)
- "In vitro breeding for salttolerancerice Callus growth in stepwise increase of salinestress and the nature of salt tolerance", pp.46~50 (1988)
- "Studies on thebiological and chemical properties of musty ginseng root andits causal mechanism", pp.24~35 (1985)
- "Rapid invitro screening of some salt tolerant bread wheats", 23(4), pp.383~389 (1995)
- "Effects of salinityon germination and seedling growth of two Atriplex species", 82, pp.162~175 (1998)
- "Salt-inducible betaine aldehydedehydrogenase from sugar beet", pp.1~11 (1992), cDNA cloning and expression
- "Alleviation of seed dormancy in thedesert forb Zygophyllum simplex L", 80, pp.395~400 (1997)
- "Studieson the optimum light intensity for growth of Panax ginseng Effects of light intensity on growth of shoots and roots ofginseng plants", pp.38~45 (1982)
- "The significanceof a two-phase growth response to salinity in wheat and barley", 22, pp.561~569 (1995)
- "A revised medium for rapid growthand bioassays with tabacco tissue culture", pp.473~479 (1962)
- "Effect of soil moisture ongrowth of Panax ginseng", pp.71~76 (1980)
- "TheSAL1 gene of Arabidopsis encoding an enzyme with 3’ increases salt tolerance in yeast", 8, pp.529~537 (1996)
- "Betaine aldehyde dehydrogenase of Sorghum", 110, pp.1301~1308 (1996)
- "Research on ginseng diseases in Korea", pp.61~68 (1993)