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논문

참돌꽃에서 Salidroside의 동정 및 현탁세포배양을 통한 분리

Identification of Salidroside from Rhodiola sachalinensis A. Bor. And its Production through Cell Suspension Culture

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Abstract

Salidroside was isolated and purified from R. sachalinensis A. Bor. roots. Purified salidroside was obtained from repeated silicagel column chromatography and preparative HPLC, and identified by 1H-NMR, 13C-NMR and 1H-1H COSY spectra analyzer. Callus induction and cell suspension from R. sachalinensis leaf segments were established on 1/2MS solid medium and in 2B5 liquid medium containing 0.5 ㎎/ℓ NAA and 1㎎/ℓ BA in the dark condition, respectively. The contents of salidroside for suspension culture were ranging from 0.12% to 0.41% in comparison with 0.17% for natural roots.

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