백두산 자생 참돌꽃의 생육과 이용. 1. 배양세포 유도와 유리아미노산 조성
Growth and Utility of Rhodiola sachalinensis in Baekdu Mountain
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Abstract
- This study was carried out to investigate the callus formation and to deter-mine the composition and contents of the free amino acids in seedlings and callus ofRhodiola sachalinensis . The callus formation from the part of seedling-roots was themost effective on MS (Murashige and Skoog) media supplemented with 2.0 mg L-1 2, 4-D included in 1.0 mg L-1 kinetin than the other experimental plots which preparedwith the different concentration of the various growth regulators. Free amino acidsextracted from the explants and the callus were a total of 25 ~ 26 kinds. Especially, thebasic amino acids such as arginine, lysine and histidine released in callus were foundrelatively in a large quantity. These results suggest that the cultured callus of Rhodiolasachalinensis could induce for the mass production of the other useful ingredients.
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