참돌꽃 (Rhodiola sachalinensis A.Bor) 현탁세포배양에서 Salidroside 생산을 위한 외재 호르몬의 영향 및 처리 농도의 최적화
Optimization of Treatment Concentration and Screening of Exogenous Plant Growth Regulators for Improvement of Salidroside Yield in Rhodiola sachalinensis A. Bor Cell Suspension Cultures
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초록
참돌꽃 (Rhodiola sachalinensis A. Bor) 현탁배양에서 salidroside 생산성을 개선하고자 auxin인 NAA와 cytokinin인 BA와 kinetin, 그 외에 GA3, TDZ, zeatin, spermine과 spermidine을 조합하여 조사하였다. NAA/BA 조합은 NAA /kinetin조합과 비교했을 때 생장과 salidroside 함량이 증가하였다. Salidroside 함량의 증가에 의한 생산성 (64.6 ± 8.9 mg/L medium)은 1 mg/L NAA와 5 mg/L BA가 첨가된 2B5 배지에서 높게 나타났으며, 최대 salidroside 의 생산성은 1 mg/L NAA, 5 mg/L BA와 1 mg/L GA3이 첨가된 2B5 배지에서 90.3 ± 8.34 mg/L로 나타났다. 그 밖의 TDZ. zeatin, spermine과 spermidine 처리에서는 생산성에 있어서 증가를 보이지 않았으며, spermidine의 경우에 약간의 유의적인 생산성 증가를 보였다.
Abstract
To enhance salidroside productivity from a cell suspension cultures of Rhodiola sachalinensis, various combinations of auxin (NAA) and cytokinins (BA, Kinetin) concentration and addition of GA3, TDZ, zeatin, spermine and spermidine at a hormone combination to be established were examined. NAA/BA combination is superior to NAA/kinetin combination in biomass and salidroside content. Maximum salidroside production (64.6± 8.9 mg/L medium) was obtained from 2B5 medium with 1 mg/L NAA and 5 mg/L BA. The adding of GA3 (0.01 mg/L∼5 mg/L) was beneficial for salidroside accumulation and the highest productivity of salidroside, 90.3 ± 8.34 mg/L, was obtained from 2B5 medium supplemented with 1 mg/L NAA, 5 mg/L BA and 0.1 mg/L GA3. On TDZ, zeatin, spermine and spermidine, expectant results were not obtained except a little affirmative effect of spermidine (69 ± 2.88 at 1 mg/L).
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