참돌꽃의 캘러스로부터 salidroside 생산에 미치는 배지성분의 영향
Effect of Macro-nutrients for the Salidroside Production from Callus Cultures of Rhodiola sachalinesis A. Bor
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Abstract
Rhodiola sachalinensis A. Bor is a Chinese herb containing the natural compound salidroside, which has been known to possess medical properties such as enhancing bodies’ ability to survive in adverse environments and extending human life. To improve a metabolite production from Rhodiola sachalinensis callus cultures, the concentrations of macro nutrients were investigated. To investigate the salidroside production in Rhodiola sachalinensis callus cultures, we analyzed salidroside content in each callus which was cultured in 30㎖ of 2 × B5 liquid medium for 4 weeks. The optimal concentrations of macro nutrients for salidroside production (67.96 ± 3.41㎎/ℓ) were found to be 99mM KNO3, 1 mM (NH4)2SO4, 2 mM NaH2PO4·2H2O, 2 mM CaCl2·2H2O and 1 mM MgSO4·7H2O. From these results, we determined the more upgraded culture condition for industrial production of salidroside.
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