생물반응기를 이용한 오미자의 현탁배양세포로부터 Gomisin J의 생산
Production of Gomisin-J from Suspension Cultured Cell of Schisandra chinensis in Airlift-type Bioreactor
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Abstract
Suspension culrute of Schisandra chinensios fro production of gomisin J was performed in bioreactor. The inoculum size and initial sucrose concentration had significant effect on the cell growth and gomisin J accumulation. The maximum dry cell weight (DCW; 43.5g/ℓ) and gomisin J content (0.71×10-³㎍/g DCW) were obtained at inoculum size of 100g fresh cell weight (FCW)per liter and MB5 medium containing 6% sucrose after 8 weeks of culture. The effect of oxygen supply on the cell growth and gomisin J accumulation was also investigated in an airlift-type bioreactor. The optimal cell growth and gomisin J content obtained in a flask cultivation.