오미자 형탁배양세포로부터 Gomisin J의 생산
Production of Gomisin J from Cell Suspension Cultures of Schisandra chinensis Baillon
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Cell growth and gomisin J production by suspension cultures of Schisandra chinensis Baillon were investigated under various culture media, initial sucrose concentrations, shaking speeds, and inoculum sizes. Callus was induced from in vitro cultivated leaf segments on MS medium supplemented with 1 ㎎/ℓ NAA. The maximum dry cell weight of 2.23 g was obtained at inoculum size of 0.5g fresh cell weight and in MB5 medium supplemented with 1 ㎎/ℓ NAA, 3% sucrose after 8 weeks. The production of gomisin J in suspension cell cultures was miximized in WPM medium containing 5% sucrose. The shaking speed for maintaining maximal cell dry weight was 100 rpm while the best shaking speed for gomisin J accumulation was 140 rpm.
Abstract
Cell growth and gomisin J production by suspension cultures of Schisandra chinensis Baillon were investigated under various culture media, initial sucrose concentrations, shaking speeds, and inoculum sizes. Callus was induced from in vitro cultivated leaf segments on MS medium supplemented with 1 ㎎/ℓ NAA. The maximum dry cell weight of 2.23 g was obtained at inoculum size of 0.5g fresh cell weight and in MB5 medium supplemented with 1 ㎎/ℓ NAA, 3% sucrose after 8 weeks. The production of gomisin J in suspension cell cultures was miximized in WPM medium containing 5% sucrose. The shaking speed for maintaining maximal cell dry weight was 100 rpm while the best shaking speed for gomisin J accumulation was 140 rpm.