오미자의 형질전환된 근으로부터 리그난 화합물의 검출

Abstract

Transformed roots of Schisandra chinensis were obtained following co-cultivation of in vitro cultivated plantlet segments with Agrobaterium rhizogens ATTCC15834. This root was examined for its growth and gomisin J contents under various culture conditions. Among the six basal cultrue media tested, WPM (Lloyd ＆ McCown, 1980) medium supplemented with 5% sucrose was the best roots growth 6.2 (g D.W/flask) and gomisin J accumulation 1.56 (X10-³ug/g D.W). Initial inoculum size correlated with the yield of biomass while gomisin J contents was not affect. Gomisin J production was influenced by the initial sucrose concentration and the highest production yield was achieved at the concentration of 7%. The optimal shaking speeds for roots growth and gomisin J production was 120 and 140 rpm, respectively.